Citation
Wang, Daijie, et al. "An Efficient Method for the Preparative Isolation and Purification of Flavonoid Glycosides and Caffeoylquinic Acid Derivatives From Leaves of Lonicera Japonica Thunb. Using High Speed Counter-Current Chromatography (HSCCC) and Prep-HPLC Guided By DPPH-HPLC Experiments." Molecules (Basel, Switzerland), vol. 22, no. 2, 2017.
Wang D, Du N, Wen L, et al. An Efficient Method for the Preparative Isolation and Purification of Flavonoid Glycosides and Caffeoylquinic Acid Derivatives from Leaves of Lonicera japonica Thunb. Using High Speed Counter-Current Chromatography (HSCCC) and Prep-HPLC Guided by DPPH-HPLC Experiments. Molecules. 2017;22(2).
Wang, D., Du, N., Wen, L., Zhu, H., Liu, F., Wang, X., Du, J., & Li, S. (2017). An Efficient Method for the Preparative Isolation and Purification of Flavonoid Glycosides and Caffeoylquinic Acid Derivatives from Leaves of Lonicera japonica Thunb. Using High Speed Counter-Current Chromatography (HSCCC) and Prep-HPLC Guided by DPPH-HPLC Experiments. Molecules (Basel, Switzerland), 22(2). https://doi.org/10.3390/molecules22020229
Wang D, et al. An Efficient Method for the Preparative Isolation and Purification of Flavonoid Glycosides and Caffeoylquinic Acid Derivatives From Leaves of Lonicera Japonica Thunb. Using High Speed Counter-Current Chromatography (HSCCC) and Prep-HPLC Guided By DPPH-HPLC Experiments. Molecules. 2017 Feb 2;22(2) PubMed PMID: 28157166.
TY - JOUR
T1 - An Efficient Method for the Preparative Isolation and Purification of Flavonoid Glycosides and Caffeoylquinic Acid Derivatives from Leaves of Lonicera japonica Thunb. Using High Speed Counter-Current Chromatography (HSCCC) and Prep-HPLC Guided by DPPH-HPLC Experiments.
AU - Wang,Daijie,
AU - Du,Ning,
AU - Wen,Lei,
AU - Zhu,Heng,
AU - Liu,Feng,
AU - Wang,Xiao,
AU - Du,Jinhua,
AU - Li,Shengbo,
Y1 - 2017/02/02/
PY - 2016/11/22/received
PY - 2017/01/27/accepted
PY - 2017/2/4/entrez
PY - 2017/2/6/pubmed
PY - 2017/5/10/medline
KW - DPPH-HPLC experiments
KW - Lonicera japonica Thunb.
KW - caffeoylquinic acid derivatives
KW - flavonoid glycosides
KW - high speed counter-current chromatography
KW - prep-HPLC combination
JF - Molecules (Basel, Switzerland)
JO - Molecules
VL - 22
IS - 2
N2 - In this work, the n-butanol extract from leaves of Lonicera japonica Thunb. (L. japonica) was reacted with DPPH and subjected to a HPLC analysis for the guided screening antioxidants (DPPH-HPLC experiments). Then, nine antioxidants, including flavonoid glycosides and caffeoylquinic acid derivatives, were isolated and purified from leaves of L. japonica using high speed counter-current chromatography (HSCCC) and prep-HPLC. The n-butanol extract was firstly isolated by HSCCC using methyl tert-butyl ether/n-butanol/acetonitrile/water (0.5% acetic acid) (2:2:1:5, v/v), yielding five fractions F1, F2 (rhoifolin), F3 (luteoloside), F4 and F5 (collected from the column after the separation). The sub-fractions F1, F4 and F5 were successfully separated by prep-HPLC. Finally, nine compounds, including chlorogenic acid (1), lonicerin (2), rutin (3), rhoifolin (4), luteoloside (5), 3,4-Odicaffeoylquinic acid (6), hyperoside (7), 3,5-O-dicaffeoylquinic acid (8), and 4,5-O-dicaffeoylquinic acid (9) were obtained, respectively, with the purities over 94% as determined by HPLC. The structures were identified by electrospray ionization mass spectrometry (ESI-MS), 1H- and 13C-NMR. Antioxidant activities were tested, and the isolated compounds showed strong antioxidant activities.
SN - 1420-3049
UR - https://www.unboundmedicine.com/medline/citation/28157166/An_Efficient_Method_for_the_Preparative_Isolation_and_Purification_of_Flavonoid_Glycosides_and_Caffeoylquinic_Acid_Derivatives_from_Leaves_of_Lonicera_japonica_Thunb__Using_High_Speed_Counter_Current_Chromatography__HSCCC__and_Prep_HPLC_Guided_by_DPPH_HPLC_Experiments_
DB - PRIME
DP - Unbound Medicine
ER -
