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Feruloyl dopamine-O-hexosides are efficient marker compounds as orthogonal validation for authentication of black cohosh (Actaea racemosa)-an UHPLC-HRAM-MS chemometrics study.
Anal Bioanal Chem. 2017 Apr; 409(10):2591-2600.AB

Abstract

Due to the complexity and variation of the chemical constituents in authentic black cohosh (Actaea racemosa) and its potential adulterant species, an accurate and feasible method for black cohosh authentication is not easy. A high-resolution accurate mass (HRAM) LC-MS fingerprinting method combined with chemometric approach was employed to discover new marker compounds. Seven hydroxycinnamic acid amide (HCAA) glycosides are proposed as potential marker compounds for differentiation of black cohosh from related species, including two Asian species (A. foetida, A. dahurica) and two American species (A. pachypoda, A. podocarpa). These markers were putatively identified by comparing their mass spectral fragmentation behavior with those of their authentic aglycone compounds and phytochemistry reports. Two isomers of feruloyl methyldopamine 4-O-hexoside ([M + H]+ 506) and one feruloyl tyramine 4-O-hexoside ([M + H]+ 476) contributed significantly to the separation of Asian species in principle component analysis (PCA) score plot. The efficacy of the models built on four reasonable combinations of these markers in differentiating black cohosh and its adulterants were evaluated and validated by partial least-square discriminant analysis (PLS-DA). Two models based on these reduced dataset achieved 100% accuracy based on the current sample collection, including the model that used only three feruloyl dopamine-O-hexoside isomers ([M + H]+ 492) and one feruloyl dopamine-O-dihexoside ([M + H-hexosyl]+ at m/z 492). Graphical abstract Hydroxycinnamic acid amide glycosides are proposed as potential marker compounds for authentication of black cohosh.

Authors+Show Affiliations

Food Composition and Methods Development Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, U.S. Department of Agriculture, 10300 Baltimore Avenue, Beltsville, MD, 20705, USA.Food Composition and Methods Development Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, U.S. Department of Agriculture, 10300 Baltimore Avenue, Beltsville, MD, 20705, USA.Food Composition and Methods Development Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, U.S. Department of Agriculture, 10300 Baltimore Avenue, Beltsville, MD, 20705, USA.Food Composition and Methods Development Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, U.S. Department of Agriculture, 10300 Baltimore Avenue, Beltsville, MD, 20705, USA.Food Composition and Methods Development Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, U.S. Department of Agriculture, 10300 Baltimore Avenue, Beltsville, MD, 20705, USA. pei.chen@ars.usda.gov.

Pub Type(s)

Journal Article
Validation Study

Language

eng

PubMed ID

28160032

Citation

Geng, Ping, et al. "Feruloyl dopamine-O-hexosides Are Efficient Marker Compounds as Orthogonal Validation for Authentication of Black Cohosh (Actaea Racemosa)-an UHPLC-HRAM-MS Chemometrics Study." Analytical and Bioanalytical Chemistry, vol. 409, no. 10, 2017, pp. 2591-2600.
Geng P, Harnly JM, Sun J, et al. Feruloyl dopamine-O-hexosides are efficient marker compounds as orthogonal validation for authentication of black cohosh (Actaea racemosa)-an UHPLC-HRAM-MS chemometrics study. Anal Bioanal Chem. 2017;409(10):2591-2600.
Geng, P., Harnly, J. M., Sun, J., Zhang, M., & Chen, P. (2017). Feruloyl dopamine-O-hexosides are efficient marker compounds as orthogonal validation for authentication of black cohosh (Actaea racemosa)-an UHPLC-HRAM-MS chemometrics study. Analytical and Bioanalytical Chemistry, 409(10), 2591-2600. https://doi.org/10.1007/s00216-017-0205-1
Geng P, et al. Feruloyl dopamine-O-hexosides Are Efficient Marker Compounds as Orthogonal Validation for Authentication of Black Cohosh (Actaea Racemosa)-an UHPLC-HRAM-MS Chemometrics Study. Anal Bioanal Chem. 2017;409(10):2591-2600. PubMed PMID: 28160032.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Feruloyl dopamine-O-hexosides are efficient marker compounds as orthogonal validation for authentication of black cohosh (Actaea racemosa)-an UHPLC-HRAM-MS chemometrics study. AU - Geng,Ping, AU - Harnly,James M, AU - Sun,Jianghao, AU - Zhang,Mengliang, AU - Chen,Pei, Y1 - 2017/02/03/ PY - 2016/09/29/received PY - 2017/01/11/accepted PY - 2016/12/28/revised PY - 2017/2/6/pubmed PY - 2018/2/24/medline PY - 2017/2/5/entrez KW - Hydroxycinnamic acid amide glycoside KW - Hydroxycinnamic acid ester KW - Nitrogen-containing KW - PCA KW - PLS-DA KW - UHPLC-HRMS SP - 2591 EP - 2600 JF - Analytical and bioanalytical chemistry JO - Anal Bioanal Chem VL - 409 IS - 10 N2 - Due to the complexity and variation of the chemical constituents in authentic black cohosh (Actaea racemosa) and its potential adulterant species, an accurate and feasible method for black cohosh authentication is not easy. A high-resolution accurate mass (HRAM) LC-MS fingerprinting method combined with chemometric approach was employed to discover new marker compounds. Seven hydroxycinnamic acid amide (HCAA) glycosides are proposed as potential marker compounds for differentiation of black cohosh from related species, including two Asian species (A. foetida, A. dahurica) and two American species (A. pachypoda, A. podocarpa). These markers were putatively identified by comparing their mass spectral fragmentation behavior with those of their authentic aglycone compounds and phytochemistry reports. Two isomers of feruloyl methyldopamine 4-O-hexoside ([M + H]+ 506) and one feruloyl tyramine 4-O-hexoside ([M + H]+ 476) contributed significantly to the separation of Asian species in principle component analysis (PCA) score plot. The efficacy of the models built on four reasonable combinations of these markers in differentiating black cohosh and its adulterants were evaluated and validated by partial least-square discriminant analysis (PLS-DA). Two models based on these reduced dataset achieved 100% accuracy based on the current sample collection, including the model that used only three feruloyl dopamine-O-hexoside isomers ([M + H]+ 492) and one feruloyl dopamine-O-dihexoside ([M + H-hexosyl]+ at m/z 492). Graphical abstract Hydroxycinnamic acid amide glycosides are proposed as potential marker compounds for authentication of black cohosh. SN - 1618-2650 UR - https://www.unboundmedicine.com/medline/citation/28160032/Feruloyl_dopamine_O_hexosides_are_efficient_marker_compounds_as_orthogonal_validation_for_authentication_of_black_cohosh__Actaea_racemosa__an_UHPLC_HRAM_MS_chemometrics_study_ L2 - https://dx.doi.org/10.1007/s00216-017-0205-1 DB - PRIME DP - Unbound Medicine ER -