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Ecl1 is a zinc-binding protein involved in the zinc-limitation-dependent extension of chronological life span in fission yeast.
Mol Genet Genomics. 2017 Apr; 292(2):475-481.MG

Abstract

Overexpression of Ecl1-family genes (ecl1 +, ecl2 +, and ecl3 +) results in the extension of the chronological life span in Schizosaccharomyces pombe. However, the mechanism for this extension has not been defined clearly. Ecl1-family proteins consist of approximately 80 amino acids, and four cysteine residues are conserved in their N-terminal domains. This study focused on the Ecl1 protein, mutating its cysteine residues sequentially to confirm their importance. As a result, all mutated Ecl1 proteins nearly lost the function to extend the chronological life span, suggesting that these four cysteine residues are essential for the Ecl1 protein. Utilizing ICP-AES (inductively coupled plasma atomic emission spectroscopy) analysis, we found that wild-type Ecl1 proteins contain zinc, while cysteine-mutated Ecl1 proteins do not. We also analyzed the effect of environmental zinc on the chronological life span. We found that zinc limitation extends the chronological life span, and this extension depends on the Ecl1-family proteins.

Authors+Show Affiliations

Laboratory of Molecular Microbiology, Department of Basic Medicinal Sciences, Graduate School of Pharmaceutical Sciences, Nagoya University, Chikusa-ku, Nagoya, 464-8601, Japan.Laboratory of Molecular Microbiology, Department of Basic Medicinal Sciences, Graduate School of Pharmaceutical Sciences, Nagoya University, Chikusa-ku, Nagoya, 464-8601, Japan.Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University, Chikusa-ku, Nagoya, 464-8601, Japan.Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University, Chikusa-ku, Nagoya, 464-8601, Japan.Laboratory of Structural Molecular Pharmacology, Department of Basic Medicinal Sciences, Graduate School of Pharmaceutical Sciences, Nagoya University, Chikusa-ku, Nagoya, 464-8601, Japan.Laboratory of Structural Molecular Pharmacology, Department of Basic Medicinal Sciences, Graduate School of Pharmaceutical Sciences, Nagoya University, Chikusa-ku, Nagoya, 464-8601, Japan.Department of Biological Science, Faculty of Science and Engineering, Chuo University, 1-13-27 Kasuga, Bunkyo-ku, Tokyo, 112-8551, Japan.Laboratory of Molecular Microbiology, Department of Basic Medicinal Sciences, Graduate School of Pharmaceutical Sciences, Nagoya University, Chikusa-ku, Nagoya, 464-8601, Japan. aiba@ps.nagoya-u.ac.jp.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28160081

Citation

Shimasaki, Takafumi, et al. "Ecl1 Is a Zinc-binding Protein Involved in the Zinc-limitation-dependent Extension of Chronological Life Span in Fission Yeast." Molecular Genetics and Genomics : MGG, vol. 292, no. 2, 2017, pp. 475-481.
Shimasaki T, Ohtsuka H, Naito C, et al. Ecl1 is a zinc-binding protein involved in the zinc-limitation-dependent extension of chronological life span in fission yeast. Mol Genet Genomics. 2017;292(2):475-481.
Shimasaki, T., Ohtsuka, H., Naito, C., Azuma, K., Tenno, T., Hiroaki, H., Murakami, H., & Aiba, H. (2017). Ecl1 is a zinc-binding protein involved in the zinc-limitation-dependent extension of chronological life span in fission yeast. Molecular Genetics and Genomics : MGG, 292(2), 475-481. https://doi.org/10.1007/s00438-016-1285-x
Shimasaki T, et al. Ecl1 Is a Zinc-binding Protein Involved in the Zinc-limitation-dependent Extension of Chronological Life Span in Fission Yeast. Mol Genet Genomics. 2017;292(2):475-481. PubMed PMID: 28160081.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ecl1 is a zinc-binding protein involved in the zinc-limitation-dependent extension of chronological life span in fission yeast. AU - Shimasaki,Takafumi, AU - Ohtsuka,Hokuto, AU - Naito,Chikako, AU - Azuma,Kenko, AU - Tenno,Takeshi, AU - Hiroaki,Hidekazu, AU - Murakami,Hiroshi, AU - Aiba,Hirofumi, Y1 - 2017/02/03/ PY - 2016/08/09/received PY - 2016/12/30/accepted PY - 2017/2/6/pubmed PY - 2017/3/30/medline PY - 2017/2/5/entrez KW - Ecl1 KW - Fission yeast KW - Life span KW - Schizosaccharomyces pombe KW - Zinc SP - 475 EP - 481 JF - Molecular genetics and genomics : MGG JO - Mol Genet Genomics VL - 292 IS - 2 N2 - Overexpression of Ecl1-family genes (ecl1 +, ecl2 +, and ecl3 +) results in the extension of the chronological life span in Schizosaccharomyces pombe. However, the mechanism for this extension has not been defined clearly. Ecl1-family proteins consist of approximately 80 amino acids, and four cysteine residues are conserved in their N-terminal domains. This study focused on the Ecl1 protein, mutating its cysteine residues sequentially to confirm their importance. As a result, all mutated Ecl1 proteins nearly lost the function to extend the chronological life span, suggesting that these four cysteine residues are essential for the Ecl1 protein. Utilizing ICP-AES (inductively coupled plasma atomic emission spectroscopy) analysis, we found that wild-type Ecl1 proteins contain zinc, while cysteine-mutated Ecl1 proteins do not. We also analyzed the effect of environmental zinc on the chronological life span. We found that zinc limitation extends the chronological life span, and this extension depends on the Ecl1-family proteins. SN - 1617-4623 UR - https://www.unboundmedicine.com/medline/citation/28160081/Ecl1_is_a_zinc_binding_protein_involved_in_the_zinc_limitation_dependent_extension_of_chronological_life_span_in_fission_yeast_ L2 - https://doi.org/10.1007/s00438-016-1285-x DB - PRIME DP - Unbound Medicine ER -