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Consensus M2e peptide conjugated to gold nanoparticles confers protection against H1N1, H3N2 and H5N1 influenza A viruses.
Antiviral Res. 2017 05; 141:62-72.AR

Abstract

The extracellular domain of influenza A ion channel membrane matrix protein 2 (M2e) is considered to be a potential candidate to develop a universal influenza A vaccine. However poor immunogenicity of M2e presents a significant roadblock. We have developed a vaccine formulation comprising of the consensus M2e peptide conjugated to gold nanoparticles (AuNPs) with CpG as a soluble adjuvant (AuNP-M2e + sCpG). We demonstrate that intranasal delivery of AuNP-M2e + sCpG in mice induces lung B cell activation and robust serum anti-M2e immunoglobulin G (IgG) response, with stimulation of both IgG1 and IgG2a subtypes. Using Madin-Darby canine kidney (MDCK) cells infected with A/California/04/2009 (H1N1pdm) pandemic strain, or A/Victoria/3/75 (H3N2), or the highly pathogenic avian influenza virus A/Vietnam/1203/2004 (H5N1) as immunosorbants we further show that the antibodies generated are also capable of binding to the homotetrameric form of M2 expressed on infected cells. Lethal challenge of vaccinated mice with A/California/04/2009 (H1N1pdm) pandemic strain, A/Victoria/3/75 (H3N2), and the highly pathogenic avian influenza virus A/Vietnam/1203/2004 (H5N1) led to 100%, 92%, and 100% protection, respectively. Overall, this study helps to lay the foundation of a potential universal influenza A vaccine.

Authors+Show Affiliations

Department of Chemical Engineering, Texas Tech University, Lubbock, TX 79409, USA.Department of Animal, Dairy and Veterinary Sciences and the School of Veterinary Medicine, Utah State University, Logan, UT 84322, USA.Department of Chemical Engineering, Texas Tech University, Lubbock, TX 79409, USA.Department of Chemical Engineering, Texas Tech University, Lubbock, TX 79409, USA.Department of Chemical Engineering, Texas Tech University, Lubbock, TX 79409, USA.Center for Human Immunobiology, Department of Pediatrics, Texas Children's Hospital, Baylor College of Medicine, Houston, TX 77030, USA.Center for Human Immunobiology, Department of Pediatrics, Texas Children's Hospital, Baylor College of Medicine, Houston, TX 77030, USA.Center for Human Immunobiology, Department of Pediatrics, Texas Children's Hospital, Baylor College of Medicine, Houston, TX 77030, USA.Center for Human Immunobiology, Department of Pediatrics, Texas Children's Hospital, Baylor College of Medicine, Houston, TX 77030, USA.Department of Animal, Dairy and Veterinary Sciences and the School of Veterinary Medicine, Utah State University, Logan, UT 84322, USA.Department of Chemical Engineering, Texas Tech University, Lubbock, TX 79409, USA. Electronic address: harvinder.gill@ttu.edu.

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

28161578

Citation

Tao, Wenqian, et al. "Consensus M2e Peptide Conjugated to Gold Nanoparticles Confers Protection Against H1N1, H3N2 and H5N1 Influenza a Viruses." Antiviral Research, vol. 141, 2017, pp. 62-72.
Tao W, Hurst BL, Shakya AK, et al. Consensus M2e peptide conjugated to gold nanoparticles confers protection against H1N1, H3N2 and H5N1 influenza A viruses. Antiviral Res. 2017;141:62-72.
Tao, W., Hurst, B. L., Shakya, A. K., Uddin, M. J., Ingrole, R. S., Hernandez-Sanabria, M., Arya, R. P., Bimler, L., Paust, S., Tarbet, E. B., & Gill, H. S. (2017). Consensus M2e peptide conjugated to gold nanoparticles confers protection against H1N1, H3N2 and H5N1 influenza A viruses. Antiviral Research, 141, 62-72. https://doi.org/10.1016/j.antiviral.2017.01.021
Tao W, et al. Consensus M2e Peptide Conjugated to Gold Nanoparticles Confers Protection Against H1N1, H3N2 and H5N1 Influenza a Viruses. Antiviral Res. 2017;141:62-72. PubMed PMID: 28161578.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Consensus M2e peptide conjugated to gold nanoparticles confers protection against H1N1, H3N2 and H5N1 influenza A viruses. AU - Tao,Wenqian, AU - Hurst,Brett L, AU - Shakya,Akhilesh Kumar, AU - Uddin,Md Jasim, AU - Ingrole,Rohan S J, AU - Hernandez-Sanabria,Mayra, AU - Arya,Ravi P, AU - Bimler,Lynn, AU - Paust,Silke, AU - Tarbet,E Bart, AU - Gill,Harvinder Singh, Y1 - 2017/02/02/ PY - 2016/07/29/received PY - 2017/01/28/revised PY - 2017/01/31/accepted PY - 2017/2/6/pubmed PY - 2017/12/20/medline PY - 2017/2/6/entrez KW - Adjuvants KW - CpG KW - Gold nanoparticles KW - Influenza vaccine KW - Intranasal vaccination KW - M2e KW - Universal influenza vaccine SP - 62 EP - 72 JF - Antiviral research JO - Antiviral Res VL - 141 N2 - The extracellular domain of influenza A ion channel membrane matrix protein 2 (M2e) is considered to be a potential candidate to develop a universal influenza A vaccine. However poor immunogenicity of M2e presents a significant roadblock. We have developed a vaccine formulation comprising of the consensus M2e peptide conjugated to gold nanoparticles (AuNPs) with CpG as a soluble adjuvant (AuNP-M2e + sCpG). We demonstrate that intranasal delivery of AuNP-M2e + sCpG in mice induces lung B cell activation and robust serum anti-M2e immunoglobulin G (IgG) response, with stimulation of both IgG1 and IgG2a subtypes. Using Madin-Darby canine kidney (MDCK) cells infected with A/California/04/2009 (H1N1pdm) pandemic strain, or A/Victoria/3/75 (H3N2), or the highly pathogenic avian influenza virus A/Vietnam/1203/2004 (H5N1) as immunosorbants we further show that the antibodies generated are also capable of binding to the homotetrameric form of M2 expressed on infected cells. Lethal challenge of vaccinated mice with A/California/04/2009 (H1N1pdm) pandemic strain, A/Victoria/3/75 (H3N2), and the highly pathogenic avian influenza virus A/Vietnam/1203/2004 (H5N1) led to 100%, 92%, and 100% protection, respectively. Overall, this study helps to lay the foundation of a potential universal influenza A vaccine. SN - 1872-9096 UR - https://www.unboundmedicine.com/medline/citation/28161578/Consensus_M2e_peptide_conjugated_to_gold_nanoparticles_confers_protection_against_H1N1_H3N2_and_H5N1_influenza_A_viruses_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0166-3542(16)30409-0 DB - PRIME DP - Unbound Medicine ER -