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LMM5.1 and LMM5.4, two eukaryotic translation elongation factor 1A-like gene family members, negatively affect cell death and disease resistance in rice.
J Genet Genomics. 2017 02 20; 44(2):107-118.JG

Abstract

Lesion mimic mutant (LMM) genes, stimulating lesion formation in the absence of pathogens, play significant roles in immune response. In this study, we characterized a rice lesion mimic mutant, lmm5, which displayed light-dependent spontaneous lesions. Additionally, lmm5 plants exhibited enhanced resistance to all of the tested races of Magnaporthe oryzae and Xanthomonas oryzae pv. oryzae (Xoo) by increasing the expression of defense-related genes and the accumulation of hydrogen peroxide. Genetic analysis showed that the lesion mimic phenotype of lmm5 was controlled by two genes, lmm5.1 and lmm5.4, which were isolated with a map-based cloning strategy. Remarkably, LMM5.1 and LMM5.4 share a 97.4% amino acid sequence identity, and they each encode a eukaryotic translation elongation factor 1A (eEF1A)-like protein. Besides, LMM5.1 and LMM5.4 were expressed in a tissue-specific and an indica-specific manner, respectively. In addition, high-throughput mRNA sequencing analysis confirmed that the basal immunity was constitutively activated in the lmm5 mutant. Taken together, these results suggest that the homologous eEF1A-like genes, LMM5.1 and LMM5.4, negatively affect cell death and disease resistance in rice.

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Authors+Show Affiliations

Zhao J
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; Kaifeng Institute for Food and Drug Control, Kaifeng 475000, China.
Liu P
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
Li C
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
Wang Y
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
Guo L
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
Jiang G
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China. Electronic address: ghjiang@genetics.ac.cn.
Zhai W
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China. Electronic address: wxzhai@genetics.ac.cn.

MeSH

Amino Acid SequenceCell DeathDisease ResistanceGene Expression Regulation, PlantMagnaportheMutationOryzaPeptide Elongation Factor 1PhenotypePlant DiseasesPlant ProteinsRNA InterferenceRNA, MessengerXanthomonas

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

28162958

Citation

Zhao, Jiying, et al. "LMM5.1 and LMM5.4, Two Eukaryotic Translation Elongation Factor 1A-like Gene Family Members, Negatively Affect Cell Death and Disease Resistance in Rice." Journal of Genetics and Genomics = Yi Chuan Xue Bao, vol. 44, no. 2, 2017, pp. 107-118.
Zhao J, Liu P, Li C, et al. LMM5.1 and LMM5.4, two eukaryotic translation elongation factor 1A-like gene family members, negatively affect cell death and disease resistance in rice. J Genet Genomics. 2017;44(2):107-118.
Zhao, J., Liu, P., Li, C., Wang, Y., Guo, L., Jiang, G., & Zhai, W. (2017). LMM5.1 and LMM5.4, two eukaryotic translation elongation factor 1A-like gene family members, negatively affect cell death and disease resistance in rice. Journal of Genetics and Genomics = Yi Chuan Xue Bao, 44(2), 107-118. https://doi.org/10.1016/j.jgg.2016.12.005
Zhao J, et al. LMM5.1 and LMM5.4, Two Eukaryotic Translation Elongation Factor 1A-like Gene Family Members, Negatively Affect Cell Death and Disease Resistance in Rice. J Genet Genomics. 2017 02 20;44(2):107-118. PubMed PMID: 28162958.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - LMM5.1 and LMM5.4, two eukaryotic translation elongation factor 1A-like gene family members, negatively affect cell death and disease resistance in rice. AU - Zhao,Jiying, AU - Liu,Pengcheng, AU - Li,Chunrong, AU - Wang,Yanyan, AU - Guo,Lequn, AU - Jiang,Guanghuai, AU - Zhai,Wenxue, Y1 - 2016/12/27/ PY - 2016/11/15/received PY - 2016/12/22/revised PY - 2016/12/26/accepted PY - 2017/2/7/pubmed PY - 2017/10/25/medline PY - 2017/2/7/entrez KW - Cell death KW - Disease resistance KW - Lesion mimic KW - Rice KW - eEF1A-like gene SP - 107 EP - 118 JF - Journal of genetics and genomics = Yi chuan xue bao JO - J Genet Genomics VL - 44 IS - 2 N2 - Lesion mimic mutant (LMM) genes, stimulating lesion formation in the absence of pathogens, play significant roles in immune response. In this study, we characterized a rice lesion mimic mutant, lmm5, which displayed light-dependent spontaneous lesions. Additionally, lmm5 plants exhibited enhanced resistance to all of the tested races of Magnaporthe oryzae and Xanthomonas oryzae pv. oryzae (Xoo) by increasing the expression of defense-related genes and the accumulation of hydrogen peroxide. Genetic analysis showed that the lesion mimic phenotype of lmm5 was controlled by two genes, lmm5.1 and lmm5.4, which were isolated with a map-based cloning strategy. Remarkably, LMM5.1 and LMM5.4 share a 97.4% amino acid sequence identity, and they each encode a eukaryotic translation elongation factor 1A (eEF1A)-like protein. Besides, LMM5.1 and LMM5.4 were expressed in a tissue-specific and an indica-specific manner, respectively. In addition, high-throughput mRNA sequencing analysis confirmed that the basal immunity was constitutively activated in the lmm5 mutant. Taken together, these results suggest that the homologous eEF1A-like genes, LMM5.1 and LMM5.4, negatively affect cell death and disease resistance in rice. SN - 1673-8527 UR - https://www.unboundmedicine.com/medline/citation/28162958/LMM5_1_and_LMM5_4_two_eukaryotic_translation_elongation_factor_1A_like_gene_family_members_negatively_affect_cell_death_and_disease_resistance_in_rice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1673-8527(16)30206-5 DB - PRIME DP - Unbound Medicine ER -