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LMM5.1 and LMM5.4, two eukaryotic translation elongation factor 1A-like gene family members, negatively affect cell death and disease resistance in rice.
J Genet Genomics. 2017 02 20; 44(2):107-118.JG

Abstract

Lesion mimic mutant (LMM) genes, stimulating lesion formation in the absence of pathogens, play significant roles in immune response. In this study, we characterized a rice lesion mimic mutant, lmm5, which displayed light-dependent spontaneous lesions. Additionally, lmm5 plants exhibited enhanced resistance to all of the tested races of Magnaporthe oryzae and Xanthomonas oryzae pv. oryzae (Xoo) by increasing the expression of defense-related genes and the accumulation of hydrogen peroxide. Genetic analysis showed that the lesion mimic phenotype of lmm5 was controlled by two genes, lmm5.1 and lmm5.4, which were isolated with a map-based cloning strategy. Remarkably, LMM5.1 and LMM5.4 share a 97.4% amino acid sequence identity, and they each encode a eukaryotic translation elongation factor 1A (eEF1A)-like protein. Besides, LMM5.1 and LMM5.4 were expressed in a tissue-specific and an indica-specific manner, respectively. In addition, high-throughput mRNA sequencing analysis confirmed that the basal immunity was constitutively activated in the lmm5 mutant. Taken together, these results suggest that the homologous eEF1A-like genes, LMM5.1 and LMM5.4, negatively affect cell death and disease resistance in rice.

Authors+Show Affiliations

Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; Kaifeng Institute for Food and Drug Control, Kaifeng 475000, China.Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China. Electronic address: ghjiang@genetics.ac.cn.Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China. Electronic address: wxzhai@genetics.ac.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

28162958

Citation

Zhao, Jiying, et al. "LMM5.1 and LMM5.4, Two Eukaryotic Translation Elongation Factor 1A-like Gene Family Members, Negatively Affect Cell Death and Disease Resistance in Rice." Journal of Genetics and Genomics = Yi Chuan Xue Bao, vol. 44, no. 2, 2017, pp. 107-118.
Zhao J, Liu P, Li C, et al. LMM5.1 and LMM5.4, two eukaryotic translation elongation factor 1A-like gene family members, negatively affect cell death and disease resistance in rice. J Genet Genomics. 2017;44(2):107-118.
Zhao, J., Liu, P., Li, C., Wang, Y., Guo, L., Jiang, G., & Zhai, W. (2017). LMM5.1 and LMM5.4, two eukaryotic translation elongation factor 1A-like gene family members, negatively affect cell death and disease resistance in rice. Journal of Genetics and Genomics = Yi Chuan Xue Bao, 44(2), 107-118. https://doi.org/10.1016/j.jgg.2016.12.005
Zhao J, et al. LMM5.1 and LMM5.4, Two Eukaryotic Translation Elongation Factor 1A-like Gene Family Members, Negatively Affect Cell Death and Disease Resistance in Rice. J Genet Genomics. 2017 02 20;44(2):107-118. PubMed PMID: 28162958.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - LMM5.1 and LMM5.4, two eukaryotic translation elongation factor 1A-like gene family members, negatively affect cell death and disease resistance in rice. AU - Zhao,Jiying, AU - Liu,Pengcheng, AU - Li,Chunrong, AU - Wang,Yanyan, AU - Guo,Lequn, AU - Jiang,Guanghuai, AU - Zhai,Wenxue, Y1 - 2016/12/27/ PY - 2016/11/15/received PY - 2016/12/22/revised PY - 2016/12/26/accepted PY - 2017/2/7/pubmed PY - 2017/10/25/medline PY - 2017/2/7/entrez KW - Cell death KW - Disease resistance KW - Lesion mimic KW - Rice KW - eEF1A-like gene SP - 107 EP - 118 JF - Journal of genetics and genomics = Yi chuan xue bao JO - J Genet Genomics VL - 44 IS - 2 N2 - Lesion mimic mutant (LMM) genes, stimulating lesion formation in the absence of pathogens, play significant roles in immune response. In this study, we characterized a rice lesion mimic mutant, lmm5, which displayed light-dependent spontaneous lesions. Additionally, lmm5 plants exhibited enhanced resistance to all of the tested races of Magnaporthe oryzae and Xanthomonas oryzae pv. oryzae (Xoo) by increasing the expression of defense-related genes and the accumulation of hydrogen peroxide. Genetic analysis showed that the lesion mimic phenotype of lmm5 was controlled by two genes, lmm5.1 and lmm5.4, which were isolated with a map-based cloning strategy. Remarkably, LMM5.1 and LMM5.4 share a 97.4% amino acid sequence identity, and they each encode a eukaryotic translation elongation factor 1A (eEF1A)-like protein. Besides, LMM5.1 and LMM5.4 were expressed in a tissue-specific and an indica-specific manner, respectively. In addition, high-throughput mRNA sequencing analysis confirmed that the basal immunity was constitutively activated in the lmm5 mutant. Taken together, these results suggest that the homologous eEF1A-like genes, LMM5.1 and LMM5.4, negatively affect cell death and disease resistance in rice. SN - 1673-8527 UR - https://www.unboundmedicine.com/medline/citation/28162958/LMM5_1_and_LMM5_4_two_eukaryotic_translation_elongation_factor_1A_like_gene_family_members_negatively_affect_cell_death_and_disease_resistance_in_rice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1673-8527(16)30206-5 DB - PRIME DP - Unbound Medicine ER -