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Response Surface Methodology Optimization of Ultrasonic-Assisted Extraction of Acer Truncatum Leaves for Maximal Phenolic Yield and Antioxidant Activity.
Molecules. 2017 Feb 04; 22(2)M

Abstract

This study is the first to report the use of response surface methodology to improve phenolic yield and antioxidant activity of Acer truncatum leaves extracts (ATLs) obtained by ultrasonic-assisted extraction. The phenolic composition in ATLs extracted under the optimized conditions were characterized by UPLC-QTOF-MS/MS. Solvent and extraction time were selected based on preliminary experiments, and a four-factors-three-levels central composite design was conducted to optimize solvent concentration (X₁), material-to-liquid ratio (X₂), ultrasonic temperature (X₃) and power (X₄) for an optimal total phenol yield (Y₁) and DPPH• antioxidant activity (Y₂). The results showed that the optimal combination was ethanol:water (v:v) 66.21%, material-to-liquid ratio 1:15.31 g/mL, ultrasonic bath temperature 60 °C, power 267.30 W, and time 30 min with three extractions, giving a maximal total phenol yield of 7593.62 mg gallic acid equivalent/100 g d.w. and a maximal DPPH• antioxidant activity of 74,241.61 μmol Trolox equivalent/100 g d.w. Furthermore, 22 phenolics were first identified in ATL extract obtained under the optimized conditions, indicating that gallates, gallotannins, quercetin, myricetin and chlorogenic acid derivatives were the main phenolic components in ATL. What's more, a gallotannins pathway existing in ATL from gallic acid to penta-O-galloylglucoside was proposed. All these results provide practical information aiming at full utilization of phenolics in ATL, together with fundamental knowledge for further research.

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Authors+Show Affiliations

Yang L
National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China. yanglingguangxdjqz@163.com.
Yin P
National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China. happy62889@126.com.
Fan H
National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China. fwqh1990@163.com.
Xue Q
National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China. xuetian20130607@163.com.
Li K
National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China. like17931@163.com.
Li X
National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China. 18289743335@163.com.
Sun L
National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China. yjliubio@bjfu.edu.cn.
Liu Y
National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China. lsun2013@bjfu.edu.cn.

MeSH

AcerAntioxidantsChromatography, High Pressure LiquidLiquid-Liquid ExtractionPhenolsPhytochemicalsPlant ExtractsPlant LeavesSolventsSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationTemperatureTime FactorsUltrasonic Waves

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28165408

Citation

Yang, Lingguang, et al. "Response Surface Methodology Optimization of Ultrasonic-Assisted Extraction of Acer Truncatum Leaves for Maximal Phenolic Yield and Antioxidant Activity." Molecules (Basel, Switzerland), vol. 22, no. 2, 2017.
Yang L, Yin P, Fan H, et al. Response Surface Methodology Optimization of Ultrasonic-Assisted Extraction of Acer Truncatum Leaves for Maximal Phenolic Yield and Antioxidant Activity. Molecules. 2017;22(2).
Yang, L., Yin, P., Fan, H., Xue, Q., Li, K., Li, X., Sun, L., & Liu, Y. (2017). Response Surface Methodology Optimization of Ultrasonic-Assisted Extraction of Acer Truncatum Leaves for Maximal Phenolic Yield and Antioxidant Activity. Molecules (Basel, Switzerland), 22(2). https://doi.org/10.3390/molecules22020232
Yang L, et al. Response Surface Methodology Optimization of Ultrasonic-Assisted Extraction of Acer Truncatum Leaves for Maximal Phenolic Yield and Antioxidant Activity. Molecules. 2017 Feb 4;22(2) PubMed PMID: 28165408.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Response Surface Methodology Optimization of Ultrasonic-Assisted Extraction of Acer Truncatum Leaves for Maximal Phenolic Yield and Antioxidant Activity. AU - Yang,Lingguang, AU - Yin,Peipei, AU - Fan,Hang, AU - Xue,Qiang, AU - Li,Ke, AU - Li,Xiang, AU - Sun,Liwei, AU - Liu,Yujun, Y1 - 2017/02/04/ PY - 2017/01/06/received PY - 2017/01/24/revised PY - 2017/01/30/accepted PY - 2017/2/7/entrez PY - 2017/2/7/pubmed PY - 2017/5/10/medline KW - Acer truncatum leaves KW - UPLC-QTOF-MS/MS KW - antioxidant activity KW - phenolics KW - response surface methodology KW - ultrasonic-assisted extraction JF - Molecules (Basel, Switzerland) JO - Molecules VL - 22 IS - 2 N2 - This study is the first to report the use of response surface methodology to improve phenolic yield and antioxidant activity of Acer truncatum leaves extracts (ATLs) obtained by ultrasonic-assisted extraction. The phenolic composition in ATLs extracted under the optimized conditions were characterized by UPLC-QTOF-MS/MS. Solvent and extraction time were selected based on preliminary experiments, and a four-factors-three-levels central composite design was conducted to optimize solvent concentration (X₁), material-to-liquid ratio (X₂), ultrasonic temperature (X₃) and power (X₄) for an optimal total phenol yield (Y₁) and DPPH• antioxidant activity (Y₂). The results showed that the optimal combination was ethanol:water (v:v) 66.21%, material-to-liquid ratio 1:15.31 g/mL, ultrasonic bath temperature 60 °C, power 267.30 W, and time 30 min with three extractions, giving a maximal total phenol yield of 7593.62 mg gallic acid equivalent/100 g d.w. and a maximal DPPH• antioxidant activity of 74,241.61 μmol Trolox equivalent/100 g d.w. Furthermore, 22 phenolics were first identified in ATL extract obtained under the optimized conditions, indicating that gallates, gallotannins, quercetin, myricetin and chlorogenic acid derivatives were the main phenolic components in ATL. What's more, a gallotannins pathway existing in ATL from gallic acid to penta-O-galloylglucoside was proposed. All these results provide practical information aiming at full utilization of phenolics in ATL, together with fundamental knowledge for further research. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/28165408/Response_Surface_Methodology_Optimization_of_Ultrasonic_Assisted_Extraction_of_Acer_Truncatum_Leaves_for_Maximal_Phenolic_Yield_and_Antioxidant_Activity_ DB - PRIME DP - Unbound Medicine ER -