Tags

Type your tag names separated by a space and hit enter

Cytogenetic study and stripe rust response of the derivatives from a wheat - Thinopyrum intermedium - Psathyrostachys huashanica trigeneric hybrid.
Genome. 2017 May; 60(5):393-401.G

Abstract

To transfer multiple desirable alien genes into common wheat, we previously reported a new trigeneric hybrid synthesized by crossing a wheat - Thinopyrum intermedium partial amphiploid with wheat - Psathyrostachys huashanica amphiploid. Here, the meiotic behavior, chromosome constitution, and stripe rust resistance of F5 derivatives from the wheat - Th. intermedium - P. huashanica trigeneric hybrid were studied. Cytological analysis indicated the F5 progenies had chromosome numbers of 42-50 (average 44.96). The mean meiotic configuration was 1.28 univalents, 21.74 bivalents, 0.04 trivalents, and 0.02 tetravalents per pollen mother cell. In 2n = 42 lines, the average pairing configuration was 0.05 I + 19.91 II (ring) + 1.06 II (rod) + 0.003 IV, suggesting these lines were cytologically stable. Most lines with 2n = 43, 44, 46, 48, or 50, bearing a high frequency of univalents or multivalents, showed abnormal meiotic behavior. Genomic in situ hybridization karyotyping results revealed that 25 lines contained 1-7 Th. intermedium chromosomes, but no P. huashanica chromosomes were found among the 27 self-pollinated progenies. At meiosis, univalents (1-5) possessing Th. intermedium hybridization signals were detected in 19 lines. Bivalents (1-3) expressing fluorescence signals were observed in 12 lines. Importantly, 21 lines harbored wheat - Th. intermedium chromosomal translocations with various alien translocation types. Additionally, two homozygous lines, K13-668-10 and K13-682-12, possessed a pair of wheat - Th. intermedium small fragmental translocations. Compared with the recurrent parent Zhong 3, most lines showed high resistance to the stripe rust (Puccinia striiformis f. sp. tritici) pathogens prevalent in China, including race V26/Gui22. This paper reports a highly efficient technical method for inducing alien translocation between wheat and Th. intermedium by trigeneric hybridization. These lines might be potentially valuable germplasm resources for further wheat improvement.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Kang HY
a Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu 611130, Sichuan, China.
Tang L
a Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu 611130, Sichuan, China.
Li DY
a Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu 611130, Sichuan, China.
Diao CD
a Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu 611130, Sichuan, China.
Zhu W
a Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu 611130, Sichuan, China.
Tang Y
a Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu 611130, Sichuan, China.
Wang Y
a Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu 611130, Sichuan, China.
Fan X
a Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu 611130, Sichuan, China.
Xu LL
a Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu 611130, Sichuan, China.
Zeng J
b College of Resources, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu 611130, Sichuan, China.
Sha LN
a Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu 611130, Sichuan, China.
Yu XF
c College of Landscape Architecture, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu 611130, Sichuan, China.
Zhang HQ
a Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu 611130, Sichuan, China.
Zhou YH
a Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu 611130, Sichuan, China.

MeSH

BasidiomycotaChromosomes, PlantCytogenetic AnalysisDisease ResistanceGenome, PlantHybridization, GeneticIn Situ HybridizationKaryotypeMeiosisPlant DiseasesPoaceaePollenTranslocation, GeneticTriticum

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28177834

Citation

Kang, Hou-Yang, et al. "Cytogenetic Study and Stripe Rust Response of the Derivatives From a Wheat - Thinopyrum Intermedium - Psathyrostachys Huashanica Trigeneric Hybrid." Genome, vol. 60, no. 5, 2017, pp. 393-401.
Kang HY, Tang L, Li DY, et al. Cytogenetic study and stripe rust response of the derivatives from a wheat - Thinopyrum intermedium - Psathyrostachys huashanica trigeneric hybrid. Genome. 2017;60(5):393-401.
Kang, H. Y., Tang, L., Li, D. Y., Diao, C. D., Zhu, W., Tang, Y., Wang, Y., Fan, X., Xu, L. L., Zeng, J., Sha, L. N., Yu, X. F., Zhang, H. Q., & Zhou, Y. H. (2017). Cytogenetic study and stripe rust response of the derivatives from a wheat - Thinopyrum intermedium - Psathyrostachys huashanica trigeneric hybrid. Genome, 60(5), 393-401. https://doi.org/10.1139/gen-2016-0135
Kang HY, et al. Cytogenetic Study and Stripe Rust Response of the Derivatives From a Wheat - Thinopyrum Intermedium - Psathyrostachys Huashanica Trigeneric Hybrid. Genome. 2017;60(5):393-401. PubMed PMID: 28177834.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cytogenetic study and stripe rust response of the derivatives from a wheat - Thinopyrum intermedium - Psathyrostachys huashanica trigeneric hybrid. AU - Kang,Hou-Yang, AU - Tang,Lin, AU - Li,Dai-Yan, AU - Diao,Cheng-Dou, AU - Zhu,Wei, AU - Tang,Yao, AU - Wang,Yi, AU - Fan,Xing, AU - Xu,Li-Li, AU - Zeng,Jian, AU - Sha,Li-Na, AU - Yu,Xiao-Fang, AU - Zhang,Hai-Qin, AU - Zhou,Yong-Hong, Y1 - 2016/12/10/ PY - 2017/2/9/pubmed PY - 2018/1/30/medline PY - 2017/2/9/entrez KW - Thinopyrum intermedium KW - genomic in situ hybridization KW - hybridation génomique in situ KW - hybride trigénérique KW - meiosis KW - méiose KW - translocation chromosome KW - translocation chromosomique KW - trigeneric hybrid SP - 393 EP - 401 JF - Genome JO - Genome VL - 60 IS - 5 N2 - To transfer multiple desirable alien genes into common wheat, we previously reported a new trigeneric hybrid synthesized by crossing a wheat - Thinopyrum intermedium partial amphiploid with wheat - Psathyrostachys huashanica amphiploid. Here, the meiotic behavior, chromosome constitution, and stripe rust resistance of F5 derivatives from the wheat - Th. intermedium - P. huashanica trigeneric hybrid were studied. Cytological analysis indicated the F5 progenies had chromosome numbers of 42-50 (average 44.96). The mean meiotic configuration was 1.28 univalents, 21.74 bivalents, 0.04 trivalents, and 0.02 tetravalents per pollen mother cell. In 2n = 42 lines, the average pairing configuration was 0.05 I + 19.91 II (ring) + 1.06 II (rod) + 0.003 IV, suggesting these lines were cytologically stable. Most lines with 2n = 43, 44, 46, 48, or 50, bearing a high frequency of univalents or multivalents, showed abnormal meiotic behavior. Genomic in situ hybridization karyotyping results revealed that 25 lines contained 1-7 Th. intermedium chromosomes, but no P. huashanica chromosomes were found among the 27 self-pollinated progenies. At meiosis, univalents (1-5) possessing Th. intermedium hybridization signals were detected in 19 lines. Bivalents (1-3) expressing fluorescence signals were observed in 12 lines. Importantly, 21 lines harbored wheat - Th. intermedium chromosomal translocations with various alien translocation types. Additionally, two homozygous lines, K13-668-10 and K13-682-12, possessed a pair of wheat - Th. intermedium small fragmental translocations. Compared with the recurrent parent Zhong 3, most lines showed high resistance to the stripe rust (Puccinia striiformis f. sp. tritici) pathogens prevalent in China, including race V26/Gui22. This paper reports a highly efficient technical method for inducing alien translocation between wheat and Th. intermedium by trigeneric hybridization. These lines might be potentially valuable germplasm resources for further wheat improvement. SN - 1480-3321 UR - https://www.unboundmedicine.com/medline/citation/28177834/Cytogenetic_study_and_stripe_rust_response_of_the_derivatives_from_a_wheat___Thinopyrum_intermedium___Psathyrostachys_huashanica_trigeneric_hybrid_ DB - PRIME DP - Unbound Medicine ER -