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Effects of diacylglycerols on the structure of phosphatidylcholine bilayers: a 2H and 31P NMR study.
Biochemistry. 1989 Sep 05; 28(18):7439-46.B

Abstract

The interaction of four diacylglycerols (DAGs) with multilamellar phospholipid bilayers consisting either of dipalmitoylphosphatidylcholine (DPPC) or of a mixture of DPPC and bovine liver phosphatidylcholine (BL-PC) extracts was investigated by a combination of 31P and 2H NMR spectrometry. We found that saturated and unsaturated long-chain DAGs induce different types of perturbations into the bilayer structure. The saturated DAGs dipalmitin and distearin induce lateral phase separation of the lipids into (i) DAG-enriched gellike domains and (ii) relatively DAG-free regions in the liquid-crystalline phase. In the latter regions, the order parameters along the fatty acyl chains of DPPC are practically identical with the control. This phase separation effect was observed in both model systems studied, and its extent is dependent upon DAG concentration and temperature. Only bilayer phases were present upon addition of dipalmitin or distearin at all concentrations and temperatures studied. The unsaturated DAGs diolein and DAG derived from egg PC (egg-DAG) affect PC bilayers in the following two ways: (i) by increasing the order parameters of the side chains, as observed for both DPPC and BL-PC model systems; (ii) by inducing nonbilayer lipid phases, as observed for BL-PC, but not DPPC. At a concentration of 25 mol % of an unsaturated DAG in mixed PC bilayers, a peak corresponding to isotropic lipid conformation appeared and increased in intensity with increase in temperature, while at 32 mol % hexagonal and bilayer phases coexisted.(ABSTRACT TRUNCATED AT 250 WORDS)

Authors+Show Affiliations

De Boeck H
Department of Biology, University of California, Riverside 92521.
Zidovetzki R
No affiliation info available

MeSH

1,2-DipalmitoylphosphatidylcholineAnimalsCattleChemical PhenomenaChemistry, PhysicalDiglyceridesGlyceridesLipid BilayersMagnetic Resonance SpectroscopyPhosphatidylcholinesTemperature

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

2819079

Citation

De Boeck, H, and R Zidovetzki. "Effects of Diacylglycerols On the Structure of Phosphatidylcholine Bilayers: a 2H and 31P NMR Study." Biochemistry, vol. 28, no. 18, 1989, pp. 7439-46.
De Boeck H, Zidovetzki R. Effects of diacylglycerols on the structure of phosphatidylcholine bilayers: a 2H and 31P NMR study. Biochemistry. 1989;28(18):7439-46.
De Boeck, H., & Zidovetzki, R. (1989). Effects of diacylglycerols on the structure of phosphatidylcholine bilayers: a 2H and 31P NMR study. Biochemistry, 28(18), 7439-46.
De Boeck H, Zidovetzki R. Effects of Diacylglycerols On the Structure of Phosphatidylcholine Bilayers: a 2H and 31P NMR Study. Biochemistry. 1989 Sep 5;28(18):7439-46. PubMed PMID: 2819079.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of diacylglycerols on the structure of phosphatidylcholine bilayers: a 2H and 31P NMR study. AU - De Boeck,H, AU - Zidovetzki,R, PY - 1989/9/5/pubmed PY - 1989/9/5/medline PY - 1989/9/5/entrez SP - 7439 EP - 46 JF - Biochemistry JO - Biochemistry VL - 28 IS - 18 N2 - The interaction of four diacylglycerols (DAGs) with multilamellar phospholipid bilayers consisting either of dipalmitoylphosphatidylcholine (DPPC) or of a mixture of DPPC and bovine liver phosphatidylcholine (BL-PC) extracts was investigated by a combination of 31P and 2H NMR spectrometry. We found that saturated and unsaturated long-chain DAGs induce different types of perturbations into the bilayer structure. The saturated DAGs dipalmitin and distearin induce lateral phase separation of the lipids into (i) DAG-enriched gellike domains and (ii) relatively DAG-free regions in the liquid-crystalline phase. In the latter regions, the order parameters along the fatty acyl chains of DPPC are practically identical with the control. This phase separation effect was observed in both model systems studied, and its extent is dependent upon DAG concentration and temperature. Only bilayer phases were present upon addition of dipalmitin or distearin at all concentrations and temperatures studied. The unsaturated DAGs diolein and DAG derived from egg PC (egg-DAG) affect PC bilayers in the following two ways: (i) by increasing the order parameters of the side chains, as observed for both DPPC and BL-PC model systems; (ii) by inducing nonbilayer lipid phases, as observed for BL-PC, but not DPPC. At a concentration of 25 mol % of an unsaturated DAG in mixed PC bilayers, a peak corresponding to isotropic lipid conformation appeared and increased in intensity with increase in temperature, while at 32 mol % hexagonal and bilayer phases coexisted.(ABSTRACT TRUNCATED AT 250 WORDS) SN - 0006-2960 UR - https://www.unboundmedicine.com/medline/citation/2819079/Effects_of_diacylglycerols_on_the_structure_of_phosphatidylcholine_bilayers:_a_2H_and_31P_NMR_study_ DB - PRIME DP - Unbound Medicine ER -