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Molecular characterisation of tumour necrosis factor alpha and its potential connection with lipoprotein lipase and peroxisome proliferator-activated receptors in blunt snout bream (Megalobrama amblycephala).
J Appl Genet 2017; 58(3):381-391JA

Abstract

Tumour necrosis factor alpha (TNF-α) is one kind of cytokines which is related to inflammation and lipid metabolism. TNF-α cDNA was cloned from the liver of blunt snout bream (Megalobrama amblycephala) through real-time polymerase chain reaction (PCR) and rapid amplification of cDNA ends (RACE) methods. The full-length cDNA of TNF-α covered 1467 bp, with an open reading frame (ORF) of 723 bp, which encodes 240 amino acids. It possessed the TNF family signature IIIPDDGIYFVYSQ. After the lipopolysaccharide (LPS) challenge test, a graded tissue-specific expression pattern of TNF-α was observed and there was high expression abundance in the kidney, brain and liver. After 8 weeks feeding trial, liver samples, two groups fed with 6% and 11% lipid levels, were collected. The results showed that, for fish fed with high-fat diet, the triglyceride of serum and lipid content of liver were elevated. Furthermore, TNF-α and peroxisome proliferator-activated receptors (PPARα, β) mRNA expression of fish fed 11% lipid diet were significantly up-regulated (p < 0.05). Lipoprotein lipase (LPL) and PPARγ mRNA expression of fish fed 11% lipid lever diet were significantly decreased compared to those of fish fed 6% (p < 0.05). The differences between the various expression of related genes in the high and low fat groups demonstrated that TNF-α played a key role in lipid metabolism, which may have an influence on fat metabolism through reducing fat synthesis and strengthening the β-oxidation of fatty acid. These discrepancies warrant further research.

Authors+Show Affiliations

Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing, 210095, Jiangsu Province, People's Republic of China.Tongwei Company Limited, No. 588 Middle Section of Tianfu Avenue, Chengdu Hi-Tech Zone, Chengdu, 610041, People's Republic of China.Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing, 210095, Jiangsu Province, People's Republic of China.Tongwei Company Limited, No. 588 Middle Section of Tianfu Avenue, Chengdu Hi-Tech Zone, Chengdu, 610041, People's Republic of China.Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing, 210095, Jiangsu Province, People's Republic of China.Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing, 210095, Jiangsu Province, People's Republic of China. jianggz@njau.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28191600

Citation

Zhou, Man, et al. "Molecular Characterisation of Tumour Necrosis Factor Alpha and Its Potential Connection With Lipoprotein Lipase and Peroxisome Proliferator-activated Receptors in Blunt Snout Bream (Megalobrama Amblycephala)." Journal of Applied Genetics, vol. 58, no. 3, 2017, pp. 381-391.
Zhou M, Mi HF, Liu WB, et al. Molecular characterisation of tumour necrosis factor alpha and its potential connection with lipoprotein lipase and peroxisome proliferator-activated receptors in blunt snout bream (Megalobrama amblycephala). J Appl Genet. 2017;58(3):381-391.
Zhou, M., Mi, H. F., Liu, W. B., Wu, Y. Y., Wang, K. Z., & Jiang, G. Z. (2017). Molecular characterisation of tumour necrosis factor alpha and its potential connection with lipoprotein lipase and peroxisome proliferator-activated receptors in blunt snout bream (Megalobrama amblycephala). Journal of Applied Genetics, 58(3), pp. 381-391. doi:10.1007/s13353-017-0390-9.
Zhou M, et al. Molecular Characterisation of Tumour Necrosis Factor Alpha and Its Potential Connection With Lipoprotein Lipase and Peroxisome Proliferator-activated Receptors in Blunt Snout Bream (Megalobrama Amblycephala). J Appl Genet. 2017;58(3):381-391. PubMed PMID: 28191600.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular characterisation of tumour necrosis factor alpha and its potential connection with lipoprotein lipase and peroxisome proliferator-activated receptors in blunt snout bream (Megalobrama amblycephala). AU - Zhou,Man, AU - Mi,Hai-Feng, AU - Liu,Wen-Bin, AU - Wu,Ye-Yang, AU - Wang,Kai-Zhou, AU - Jiang,Guang-Zhen, Y1 - 2017/02/13/ PY - 2016/08/03/received PY - 2017/01/26/accepted PY - 2017/01/02/revised PY - 2017/2/14/pubmed PY - 2017/8/15/medline PY - 2017/2/14/entrez KW - Gene expression KW - Lipoprotein lipase (LPL) KW - Megalobrama amblycephala KW - Peroxisome proliferator-activated receptors (PPARs) KW - Tumour necrosis factor alpha (TNF-α) SP - 381 EP - 391 JF - Journal of applied genetics JO - J. Appl. Genet. VL - 58 IS - 3 N2 - Tumour necrosis factor alpha (TNF-α) is one kind of cytokines which is related to inflammation and lipid metabolism. TNF-α cDNA was cloned from the liver of blunt snout bream (Megalobrama amblycephala) through real-time polymerase chain reaction (PCR) and rapid amplification of cDNA ends (RACE) methods. The full-length cDNA of TNF-α covered 1467 bp, with an open reading frame (ORF) of 723 bp, which encodes 240 amino acids. It possessed the TNF family signature IIIPDDGIYFVYSQ. After the lipopolysaccharide (LPS) challenge test, a graded tissue-specific expression pattern of TNF-α was observed and there was high expression abundance in the kidney, brain and liver. After 8 weeks feeding trial, liver samples, two groups fed with 6% and 11% lipid levels, were collected. The results showed that, for fish fed with high-fat diet, the triglyceride of serum and lipid content of liver were elevated. Furthermore, TNF-α and peroxisome proliferator-activated receptors (PPARα, β) mRNA expression of fish fed 11% lipid diet were significantly up-regulated (p < 0.05). Lipoprotein lipase (LPL) and PPARγ mRNA expression of fish fed 11% lipid lever diet were significantly decreased compared to those of fish fed 6% (p < 0.05). The differences between the various expression of related genes in the high and low fat groups demonstrated that TNF-α played a key role in lipid metabolism, which may have an influence on fat metabolism through reducing fat synthesis and strengthening the β-oxidation of fatty acid. These discrepancies warrant further research. SN - 2190-3883 UR - https://www.unboundmedicine.com/medline/citation/28191600/Molecular_characterisation_of_tumour_necrosis_factor_alpha_and_its_potential_connection_with_lipoprotein_lipase_and_peroxisome_proliferator_activated_receptors_in_blunt_snout_bream__Megalobrama_amblycephala__ L2 - https://dx.doi.org/10.1007/s13353-017-0390-9 DB - PRIME DP - Unbound Medicine ER -