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Hair growth-promoting effect of Geranium sibiricum extract in human dermal papilla cells and C57BL/6 mice.
BMC Complement Altern Med. 2017 Feb 13; 17(1):109.BC

Abstract

BACKGROUND

Geranium sibiricum L. has been used as a medicinal plant to treat diarrhea, bacterial infection, and cancer in Bulgaria, Peru, and Korea. However, its hair growth-promoting effect was not investigated so far. This study examined the effects of Geranium sibiricum L. extract (GSE) on hair growth, using in vitro and in vivo models.

METHODS

Antioxidant, proliferation and migration assay of GSE was performed with human dermal papilla cells (hDPCs). Hair-growth promoting effect was measured in animal model. Relative expression of interleukin-1, vascular endothelial growth factor, hepatocyte growth factor, and transforming growth factor beta 1 was determined by real time RT-PCR. Expression of Ki-67 and stem cell factor were analyzed by immunohistochemistry.

RESULTS

GSE treatment proliferated and migrated human dermal papilla cells (hDPCs) more than treatment of 10 μM minoxidil. GSE significantly stimulated the expression of Ki-67 protein and the mRNA levels of hepatocyte growth factor and vascular endothelial growth factor in hDPCs. Topical application of 1,000 ppm GSE for 3 weeks promoted more significant hair growth on shaved C57BL/6 mice than did 5% minoxidil. The histological morphology of hair follicles demonstrated an active anagen phase with the induction of stem cell factor. GSE treatment significantly reduced the number of mast cells and the expression of transforming growth factor beta 1 in mouse skin tissues.

CONCLUSIONS

These results demonstrated that GSE promotes hair growth in vitro and in vivo by regulating growth factors and the cellular response.

Authors+Show Affiliations

John A. Burns School of Medicine, University of Hawaii, Honolulu, HI, 96813, USA.Department of Food and Nutrition, College of Biotechnology and Natural Resources, Chung-Ang University, Anseong, 17546, Korea.Department of Food and Nutrition, College of Biotechnology and Natural Resources, Chung-Ang University, Anseong, 17546, Korea.Department of Food and Nutrition, College of Biotechnology and Natural Resources, Chung-Ang University, Anseong, 17546, Korea.Department of Food and Nutrition, College of Biotechnology and Natural Resources, Chung-Ang University, Anseong, 17546, Korea.Department of Integrative Plant Science, College of Biotechnology and Natural Resources, Chung-Ang University, Anseong, 17546, Korea.Department of Food and Nutrition, College of Biotechnology and Natural Resources, Chung-Ang University, Anseong, 17546, Korea.Department of Integrative Plant Science, College of Biotechnology and Natural Resources, Chung-Ang University, Anseong, 17546, Korea.Department of Food and Nutrition, College of Biotechnology and Natural Resources, Chung-Ang University, Anseong, 17546, Korea. lbheelb@cau.ac.kr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28193226

Citation

Boisvert, William A., et al. "Hair Growth-promoting Effect of Geranium Sibiricum Extract in Human Dermal Papilla Cells and C57BL/6 Mice." BMC Complementary and Alternative Medicine, vol. 17, no. 1, 2017, p. 109.
Boisvert WA, Yu M, Choi Y, et al. Hair growth-promoting effect of Geranium sibiricum extract in human dermal papilla cells and C57BL/6 mice. BMC Complement Altern Med. 2017;17(1):109.
Boisvert, W. A., Yu, M., Choi, Y., Jeong, G. H., Zhang, Y. L., Cho, S., Choi, C., Lee, S., & Lee, B. H. (2017). Hair growth-promoting effect of Geranium sibiricum extract in human dermal papilla cells and C57BL/6 mice. BMC Complementary and Alternative Medicine, 17(1), 109. https://doi.org/10.1186/s12906-017-1624-4
Boisvert WA, et al. Hair Growth-promoting Effect of Geranium Sibiricum Extract in Human Dermal Papilla Cells and C57BL/6 Mice. BMC Complement Altern Med. 2017 Feb 13;17(1):109. PubMed PMID: 28193226.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hair growth-promoting effect of Geranium sibiricum extract in human dermal papilla cells and C57BL/6 mice. AU - Boisvert,William A, AU - Yu,Miri, AU - Choi,Youngbin, AU - Jeong,Gi Hee, AU - Zhang,Yi-Lin, AU - Cho,Sunghun, AU - Choi,Changsun, AU - Lee,Sanghyun, AU - Lee,Bog-Hieu, Y1 - 2017/02/13/ PY - 2016/06/30/received PY - 2017/02/05/accepted PY - 2017/2/15/entrez PY - 2017/2/15/pubmed PY - 2017/2/17/medline KW - Antioxidant KW - Geranium sibiricum L KW - Hair loss KW - Human dermal papilla cells KW - Transforming growth factor beta 1 SP - 109 EP - 109 JF - BMC complementary and alternative medicine JO - BMC Complement Altern Med VL - 17 IS - 1 N2 - BACKGROUND: Geranium sibiricum L. has been used as a medicinal plant to treat diarrhea, bacterial infection, and cancer in Bulgaria, Peru, and Korea. However, its hair growth-promoting effect was not investigated so far. This study examined the effects of Geranium sibiricum L. extract (GSE) on hair growth, using in vitro and in vivo models. METHODS: Antioxidant, proliferation and migration assay of GSE was performed with human dermal papilla cells (hDPCs). Hair-growth promoting effect was measured in animal model. Relative expression of interleukin-1, vascular endothelial growth factor, hepatocyte growth factor, and transforming growth factor beta 1 was determined by real time RT-PCR. Expression of Ki-67 and stem cell factor were analyzed by immunohistochemistry. RESULTS: GSE treatment proliferated and migrated human dermal papilla cells (hDPCs) more than treatment of 10 μM minoxidil. GSE significantly stimulated the expression of Ki-67 protein and the mRNA levels of hepatocyte growth factor and vascular endothelial growth factor in hDPCs. Topical application of 1,000 ppm GSE for 3 weeks promoted more significant hair growth on shaved C57BL/6 mice than did 5% minoxidil. The histological morphology of hair follicles demonstrated an active anagen phase with the induction of stem cell factor. GSE treatment significantly reduced the number of mast cells and the expression of transforming growth factor beta 1 in mouse skin tissues. CONCLUSIONS: These results demonstrated that GSE promotes hair growth in vitro and in vivo by regulating growth factors and the cellular response. SN - 1472-6882 UR - https://www.unboundmedicine.com/medline/citation/28193226/Hair_growth_promoting_effect_of_Geranium_sibiricum_extract_in_human_dermal_papilla_cells_and_C57BL/6_mice_ L2 - https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/s12906-017-1624-4 DB - PRIME DP - Unbound Medicine ER -