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Exploring the Role of TRPV and CGRP in Adenosine Preconditioning and Remote Hind Limb Preconditioning-Induced Cardioprotection in Rats.
Cardiovasc Drugs Ther. 2017 Apr; 31(2):133-143.CD

Abstract

The cardioprotective effects of remote hind limb preconditioning (RIPC) are well known, but mechanisms by which protection occurs still remain to be explored. Therefore, the present study was designed to investigate the role of TRPV and CGRP in adenosine and remote preconditioning-induced cardioprotection, using sumatriptan, a CGRP release inhibitor and ruthenium red, a TRPV inhibitor, in rats. For remote preconditioning, a pressure cuff was tied around the hind limb of the rat and was inflated with air up to 150 mmHg to produce ischemia in the hind limb and during reperfusion pressure was released. Four cycles of ischemia and reperfusion, each consisting of 5 min of inflation and 5 min of deflation of pressure cuff were used to produce remote limb preconditioning. An ex vivo Langendorff's isolated rat heart model was used to induce ischemia reperfusion injury by 30 min of global ischemia followed by 120 min of reperfusion. RIPC demonstrated a significant decrease in ischemia reperfusion-induced significant myocardial injury in terms of increase in LDH, CK, infarct size and decrease in LVDP, +dp/dtmax and -dp/dtmin. Moreover, pharmacological preconditioning with adenosine produced cardioprotective effects in a similar manner to RIPC. Pretreatment with sumatriptan, a CGRP release blocker, abolished RIPC and adenosine preconditioning-induced cardioprotective effects. Administration of ruthenium red, a TRPV inhibitor, also abolished adenosine preconditioning-induced cardioprotection. It may be proposed that the cardioprotective effects of adenosine and remote preconditioning are possibly mediated through activation of a TRPV channels and consequent, release of CGRP.

Authors+Show Affiliations

Department of Pharmaceutical Sciences and Drug Research, Punjabi University Patiala, Patiala, 147002, India.Department of Pharmaceutical Sciences and Drug Research, Punjabi University Patiala, Patiala, 147002, India.Department of Pharmaceutical Sciences and Drug Research, Punjabi University Patiala, Patiala, 147002, India.Department of Pharmaceutical Sciences and Drug Research, Punjabi University Patiala, Patiala, 147002, India.Department of Pharmaceutical Sciences and Drug Research, Punjabi University Patiala, Patiala, 147002, India. amteshwarjaggi@yahoo.co.in.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28194544

Citation

Singh, Amritpal, et al. "Exploring the Role of TRPV and CGRP in Adenosine Preconditioning and Remote Hind Limb Preconditioning-Induced Cardioprotection in Rats." Cardiovascular Drugs and Therapy, vol. 31, no. 2, 2017, pp. 133-143.
Singh A, Randhawa PK, Bali A, et al. Exploring the Role of TRPV and CGRP in Adenosine Preconditioning and Remote Hind Limb Preconditioning-Induced Cardioprotection in Rats. Cardiovasc Drugs Ther. 2017;31(2):133-143.
Singh, A., Randhawa, P. K., Bali, A., Singh, N., & Jaggi, A. S. (2017). Exploring the Role of TRPV and CGRP in Adenosine Preconditioning and Remote Hind Limb Preconditioning-Induced Cardioprotection in Rats. Cardiovascular Drugs and Therapy, 31(2), 133-143. https://doi.org/10.1007/s10557-017-6716-3
Singh A, et al. Exploring the Role of TRPV and CGRP in Adenosine Preconditioning and Remote Hind Limb Preconditioning-Induced Cardioprotection in Rats. Cardiovasc Drugs Ther. 2017;31(2):133-143. PubMed PMID: 28194544.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Exploring the Role of TRPV and CGRP in Adenosine Preconditioning and Remote Hind Limb Preconditioning-Induced Cardioprotection in Rats. AU - Singh,Amritpal, AU - Randhawa,Puneet Kaur, AU - Bali,Anjana, AU - Singh,Nirmal, AU - Jaggi,Amteshwar Singh, PY - 2017/2/15/pubmed PY - 2018/1/6/medline PY - 2017/2/15/entrez KW - Adenosine preconditioning KW - CGRP KW - Cardioprotection KW - Remote ischemic preconditioning KW - Ruthenium red KW - Sumatriptan KW - TRPV1 channels SP - 133 EP - 143 JF - Cardiovascular drugs and therapy JO - Cardiovasc Drugs Ther VL - 31 IS - 2 N2 - The cardioprotective effects of remote hind limb preconditioning (RIPC) are well known, but mechanisms by which protection occurs still remain to be explored. Therefore, the present study was designed to investigate the role of TRPV and CGRP in adenosine and remote preconditioning-induced cardioprotection, using sumatriptan, a CGRP release inhibitor and ruthenium red, a TRPV inhibitor, in rats. For remote preconditioning, a pressure cuff was tied around the hind limb of the rat and was inflated with air up to 150 mmHg to produce ischemia in the hind limb and during reperfusion pressure was released. Four cycles of ischemia and reperfusion, each consisting of 5 min of inflation and 5 min of deflation of pressure cuff were used to produce remote limb preconditioning. An ex vivo Langendorff's isolated rat heart model was used to induce ischemia reperfusion injury by 30 min of global ischemia followed by 120 min of reperfusion. RIPC demonstrated a significant decrease in ischemia reperfusion-induced significant myocardial injury in terms of increase in LDH, CK, infarct size and decrease in LVDP, +dp/dtmax and -dp/dtmin. Moreover, pharmacological preconditioning with adenosine produced cardioprotective effects in a similar manner to RIPC. Pretreatment with sumatriptan, a CGRP release blocker, abolished RIPC and adenosine preconditioning-induced cardioprotective effects. Administration of ruthenium red, a TRPV inhibitor, also abolished adenosine preconditioning-induced cardioprotection. It may be proposed that the cardioprotective effects of adenosine and remote preconditioning are possibly mediated through activation of a TRPV channels and consequent, release of CGRP. SN - 1573-7241 UR - https://www.unboundmedicine.com/medline/citation/28194544/Exploring_the_Role_of_TRPV_and_CGRP_in_Adenosine_Preconditioning_and_Remote_Hind_Limb_Preconditioning_Induced_Cardioprotection_in_Rats_ L2 - https://doi.org/10.1007/s10557-017-6716-3 DB - PRIME DP - Unbound Medicine ER -