Double-stranded RNA delivery through soaking mediates silencing of the muscle protein 20 and increases mortality to the Asian citrus psyllid, Diaphorina citri.
Pest Manag Sci. 2017 Sep; 73(9):1846-1853.PM

Abstract

BACKGROUND

Asian citrus psyllid, Diaphorina citri Kuwayama, is the most important economic pest of citrus because it transmits Candidatus Liberibacter asiaticus (CLas), the causal agent of huanglongbing (HLB). Silencing genes by RNA interference (RNAi) is a promising approach for controlling D. citri. RNAi-based insect management strategies depend on the selection of suitable target genes.

RESULTS

The muscle protein 20 gene DcMP20 was characterized from D. citri in an effort to impair proper muscle development through RNAi. Phylogenetic analysis showed that DcMP20 was more closely related to MP20 from Drosophila compared with its counterpart from other insect species. Developmental expression analysis revealed that transcription of DcMP20 was development dependent and reached a maximum level in the last instar (fourth-fifth) of the nymphal stage. The extent of RNAi in D. citri was dose dependent, with dsRNA-DcMP20 at 75 ng µL-1 being sufficient to knock down endogenous DcMP20 expression, which resulted in significant mortality and reduced body weight that positively correlated with the silencing of DcMP20. No effect was found when dsRNA-GFP or water was used, indicating the specific effect of dsRNA-DcMP20.

CONCLUSION

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Publisher Full Text (DOI)

Authors+Show Affiliations

Yu X

Citrus Research and Education Center, IFAS, University of Florida, Lake Alfred, FL, USA. School of Agricultural Engineering/Henan Collaborative Innovation Center of Water Security for Water Source Region of Mid-line of South-to-North Diversion Project, Nanyang Normal University, Nanyang, Henan, China.

Gowda S

Citrus Research and Education Center, IFAS, University of Florida, Lake Alfred, FL, USA.

Killiny N

Citrus Research and Education Center, IFAS, University of Florida, Lake Alfred, FL, USA.

MeSH

Amino Acid SequenceAnimalsBody WeightDrug Delivery SystemsGene Expression Regulation, DevelopmentalGene SilencingHemipteraInsect ProteinsMuscle ProteinsRNA, Double-Stranded

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28195429

Citation

Yu, Xiudao, et al. "Double-stranded RNA Delivery Through Soaking Mediates Silencing of the Muscle Protein 20 and Increases Mortality to the Asian Citrus Psyllid, Diaphorina Citri." Pest Management Science, vol. 73, no. 9, 2017, pp. 1846-1853.

Yu X, Gowda S, Killiny N. Double-stranded RNA delivery through soaking mediates silencing of the muscle protein 20 and increases mortality to the Asian citrus psyllid, Diaphorina citri. Pest Manag Sci. 2017;73(9):1846-1853.

Yu, X., Gowda, S., & Killiny, N. (2017). Double-stranded RNA delivery through soaking mediates silencing of the muscle protein 20 and increases mortality to the Asian citrus psyllid, Diaphorina citri. Pest Management Science, 73(9), 1846-1853. https://doi.org/10.1002/ps.4549

Yu X, Gowda S, Killiny N. Double-stranded RNA Delivery Through Soaking Mediates Silencing of the Muscle Protein 20 and Increases Mortality to the Asian Citrus Psyllid, Diaphorina Citri. Pest Manag Sci. 2017;73(9):1846-1853. PubMed PMID: 28195429.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Double-stranded RNA delivery through soaking mediates silencing of the muscle protein 20 and increases mortality to the Asian citrus psyllid, Diaphorina citri. AU - Yu,Xiudao, AU - Gowda,Siddarame, AU - Killiny,Nabil, Y1 - 2017/03/14/ PY - 2016/06/09/received PY - 2017/01/26/revised PY - 2017/02/07/accepted PY - 2017/2/15/pubmed PY - 2018/3/31/medline PY - 2017/2/15/entrez KW - Asian citrus psyllid KW - RNA interference KW - calponin KW - muscle protein 20 KW - nymph mortality KW - soaking delivery SP - 1846 EP - 1853 JF - Pest management science JO - Pest Manag Sci VL - 73 IS - 9 N2 - BACKGROUND: Asian citrus psyllid, Diaphorina citri Kuwayama, is the most important economic pest of citrus because it transmits Candidatus Liberibacter asiaticus (CLas), the causal agent of huanglongbing (HLB). Silencing genes by RNA interference (RNAi) is a promising approach for controlling D. citri. RNAi-based insect management strategies depend on the selection of suitable target genes. RESULTS: The muscle protein 20 gene DcMP20 was characterized from D. citri in an effort to impair proper muscle development through RNAi. Phylogenetic analysis showed that DcMP20 was more closely related to MP20 from Drosophila compared with its counterpart from other insect species. Developmental expression analysis revealed that transcription of DcMP20 was development dependent and reached a maximum level in the last instar (fourth-fifth) of the nymphal stage. The extent of RNAi in D. citri was dose dependent, with dsRNA-DcMP20 at 75 ng µL-1 being sufficient to knock down endogenous DcMP20 expression, which resulted in significant mortality and reduced body weight that positively correlated with the silencing of DcMP20. No effect was found when dsRNA-GFP or water was used, indicating the specific effect of dsRNA-DcMP20. CONCLUSION: Our results suggest that dsRNA can be delivered to D. citri through soaking, and DcMP20 is an effective RNAi target to be used in the management of D. citri. © 2017 Society of Chemical Industry. SN - 1526-4998 UR - https://www.unboundmedicine.com/medline/citation/28195429/Double_stranded_RNA_delivery_through_soaking_mediates_silencing_of_the_muscle_protein_20_and_increases_mortality_to_the_Asian_citrus_psyllid_Diaphorina_citri_ L2 - https://doi.org/10.1002/ps.4549 DB - PRIME DP - Unbound Medicine ER -

Tags

Double-stranded RNA delivery through soaking mediates silencing of the muscle protein 20 and increases mortality to the Asian citrus psyllid, Diaphorina citri.Pest Manag Sci. 2017 Sep; 73(9):1846-1853.PM