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SPL33, encoding an eEF1A-like protein, negatively regulates cell death and defense responses in rice.
J Exp Bot. 2017 02 01; 68(5):899-913.JE

Abstract

Lesion-mimic mutants are useful to dissect programmed cell death and defense-related pathways in plants. Here we identified a new rice lesion-mimic mutant, spotted leaf 33 (spl33) and cloned the causal gene by a map-based cloning strategy. SPL33 encodes a eukaryotic translation elongation factor 1 alpha (eEF1A)-like protein consisting of a non-functional zinc finger domain and three functional EF-Tu domains. spl33 exhibited programmed cell death-mediated cell death and early leaf senescence, as evidenced by analyses of four histochemical markers, namely H2O2 accumulation, cell death, callose accumulation and TUNEL-positive nuclei, and by four indicators, namely loss of chlorophyll, breakdown of chloroplasts, down-regulation of photosynthesis-related genes, and up-regulation of senescence-associated genes. Defense responses were induced in the spl33 mutant, as shown by enhanced resistance to both the fungal pathogen Magnaporthe oryzae and the bacterial pathogen Xanthomonas oryzae pv. oryzae and by up-regulation of defense response genes. Transcriptome analysis of the spl33 mutant and its wild type provided further evidence for the biological effects of loss of SPL33 function in cell death, leaf senescence and defense responses in rice. Detailed analyses showed that reactive oxygen species accumulation may be the cause of cell death in the spl33 mutant, whereas uncontrolled activation of multiple innate immunity-related receptor genes and signaling molecules may be responsible for the enhanced disease resistance observed in spl33. Thus, we have demonstrated involvement of an eEF1A-like protein in programmed cell death and provided a link to defense responses in rice.

Authors+Show Affiliations

Institute of Crop Science, Chinese Academy of Agriculture Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081, China.Institute of Crop Science, Chinese Academy of Agriculture Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081, China.Institute of Crop Science, Chinese Academy of Agriculture Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081, China.Institute of Crop Science, Chinese Academy of Agriculture Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081, China.Institute of Crop Science, Chinese Academy of Agriculture Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081, China.Institute of Crop Science, Chinese Academy of Agriculture Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081, China.Institute of Crop Science, Chinese Academy of Agriculture Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081, China.Institute of Crop Science, Chinese Academy of Agriculture Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081, China.Key Laboratory of Crop Genetics and Germplasm Enhancement/Jiangsu Provincial Center of Plant Gene Engineering, Nanjing Agricultural University, Nanjing 210095, China.Institute of Crop Science, Chinese Academy of Agriculture Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081, China.Institute of Crop Science, Chinese Academy of Agriculture Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081, China.Institute of Crop Science, Chinese Academy of Agriculture Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081, China.Institute of Crop Science, Chinese Academy of Agriculture Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081, China.Key Laboratory of Crop Genetics and Germplasm Enhancement/Jiangsu Provincial Center of Plant Gene Engineering, Nanjing Agricultural University, Nanjing 210095, China.Institute of Crop Science, Chinese Academy of Agriculture Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081, China.Institute of Crop Science, Chinese Academy of Agriculture Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081, China. Key Laboratory of Crop Genetics and Germplasm Enhancement/Jiangsu Provincial Center of Plant Gene Engineering, Nanjing Agricultural University, Nanjing 210095, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

28199670

Citation

Wang, Shuai, et al. "SPL33, Encoding an eEF1A-like Protein, Negatively Regulates Cell Death and Defense Responses in Rice." Journal of Experimental Botany, vol. 68, no. 5, 2017, pp. 899-913.
Wang S, Lei C, Wang J, et al. SPL33, encoding an eEF1A-like protein, negatively regulates cell death and defense responses in rice. J Exp Bot. 2017;68(5):899-913.
Wang, S., Lei, C., Wang, J., Ma, J., Tang, S., Wang, C., Zhao, K., Tian, P., Zhang, H., Qi, C., Cheng, Z., Zhang, X., Guo, X., Liu, L., Wu, C., & Wan, J. (2017). SPL33, encoding an eEF1A-like protein, negatively regulates cell death and defense responses in rice. Journal of Experimental Botany, 68(5), 899-913. https://doi.org/10.1093/jxb/erx001
Wang S, et al. SPL33, Encoding an eEF1A-like Protein, Negatively Regulates Cell Death and Defense Responses in Rice. J Exp Bot. 2017 02 1;68(5):899-913. PubMed PMID: 28199670.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - SPL33, encoding an eEF1A-like protein, negatively regulates cell death and defense responses in rice. AU - Wang,Shuai, AU - Lei,Cailin, AU - Wang,Jiulin, AU - Ma,Jian, AU - Tang,Sha, AU - Wang,Chunlian, AU - Zhao,Kaijun, AU - Tian,Peng, AU - Zhang,Huan, AU - Qi,Changyan, AU - Cheng,Zhijun, AU - Zhang,Xin, AU - Guo,Xiuping, AU - Liu,Linglong, AU - Wu,Chuanyin, AU - Wan,Jianmin, PY - 2017/2/16/pubmed PY - 2017/11/29/medline PY - 2017/2/16/entrez KW - Defense responses KW - Oryza sativa KW - SPL33. KW - eukaryotic translation elongation factor 1 alpha (eEF1A) KW - lesion-mimic mutant KW - programed cell death SP - 899 EP - 913 JF - Journal of experimental botany JO - J Exp Bot VL - 68 IS - 5 N2 - Lesion-mimic mutants are useful to dissect programmed cell death and defense-related pathways in plants. Here we identified a new rice lesion-mimic mutant, spotted leaf 33 (spl33) and cloned the causal gene by a map-based cloning strategy. SPL33 encodes a eukaryotic translation elongation factor 1 alpha (eEF1A)-like protein consisting of a non-functional zinc finger domain and three functional EF-Tu domains. spl33 exhibited programmed cell death-mediated cell death and early leaf senescence, as evidenced by analyses of four histochemical markers, namely H2O2 accumulation, cell death, callose accumulation and TUNEL-positive nuclei, and by four indicators, namely loss of chlorophyll, breakdown of chloroplasts, down-regulation of photosynthesis-related genes, and up-regulation of senescence-associated genes. Defense responses were induced in the spl33 mutant, as shown by enhanced resistance to both the fungal pathogen Magnaporthe oryzae and the bacterial pathogen Xanthomonas oryzae pv. oryzae and by up-regulation of defense response genes. Transcriptome analysis of the spl33 mutant and its wild type provided further evidence for the biological effects of loss of SPL33 function in cell death, leaf senescence and defense responses in rice. Detailed analyses showed that reactive oxygen species accumulation may be the cause of cell death in the spl33 mutant, whereas uncontrolled activation of multiple innate immunity-related receptor genes and signaling molecules may be responsible for the enhanced disease resistance observed in spl33. Thus, we have demonstrated involvement of an eEF1A-like protein in programmed cell death and provided a link to defense responses in rice. SN - 1460-2431 UR - https://www.unboundmedicine.com/medline/citation/28199670/SPL33_encoding_an_eEF1A_like_protein_negatively_regulates_cell_death_and_defense_responses_in_rice_ L2 - https://academic.oup.com/jxb/article-lookup/doi/10.1093/jxb/erx001 DB - PRIME DP - Unbound Medicine ER -