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Isolation of four phenolic compounds from Mangifera indica L. flowers by using normal phase combined with elution extrusion two-step high speed countercurrent chromatography.
J Chromatogr B Analyt Technol Biomed Life Sci. 2017 Mar 01; 1046:211-217.JC

Abstract

Two-step high speed countercurrent chromatography method, following normal phase and elution-extrusion mode of operation by using selected solvent systems, was introduced for phenolic compounds separation. Phenolic compounds including gallic acid, ethyl gallate, ethyl digallate and ellagic acid were separated from the ethanol extract of mango (Mangifera indica L.) flowers for the first time. In the first step, gallic acid of 3.7mg and ethyl gallate of 3.9mg with the purities of 98.87% and 99.55%, respectively, were isolated by using hexane-ethylacetate-methanol-water (4:6:4:6, v/v) in normal phase high speed countercurrent chromatography from 200mg of crude extract, while ethyl digallate and ellagic acid were collected in the form of mixture fraction. In the second step, further purification of the mixture was carried out with the help of another selected solvent system of dichloromethane-methanol-water (4:3:2, v/v) following elusion-extrusion mode of operation. Ethyl digallate of 3.8mg and ellagic acid of 5.7mg were separated well with high purities of 98.68% and 99.71%, respectively. The separated phenolic compounds were identified and confirmed by HPLC, UPLC-QTOF/ESI-MS, 1H and 13C NMR spectrometric analysis.

Authors+Show Affiliations

State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, 15 3rd North East Road, Chaoyang District, Beijing 100029, China.State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, 15 3rd North East Road, Chaoyang District, Beijing 100029, China.State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, 15 3rd North East Road, Chaoyang District, Beijing 100029, China.State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, 15 3rd North East Road, Chaoyang District, Beijing 100029, China.State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, 15 3rd North East Road, Chaoyang District, Beijing 100029, China. Electronic address: weiyun@mail.buct.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28199916

Citation

Shaheen, Nusrat, et al. "Isolation of Four Phenolic Compounds From Mangifera Indica L. Flowers By Using Normal Phase Combined With Elution Extrusion Two-step High Speed Countercurrent Chromatography." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 1046, 2017, pp. 211-217.
Shaheen N, Lu Y, Geng P, et al. Isolation of four phenolic compounds from Mangifera indica L. flowers by using normal phase combined with elution extrusion two-step high speed countercurrent chromatography. J Chromatogr B Analyt Technol Biomed Life Sci. 2017;1046:211-217.
Shaheen, N., Lu, Y., Geng, P., Shao, Q., & Wei, Y. (2017). Isolation of four phenolic compounds from Mangifera indica L. flowers by using normal phase combined with elution extrusion two-step high speed countercurrent chromatography. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 1046, 211-217. https://doi.org/10.1016/j.jchromb.2017.01.018
Shaheen N, et al. Isolation of Four Phenolic Compounds From Mangifera Indica L. Flowers By Using Normal Phase Combined With Elution Extrusion Two-step High Speed Countercurrent Chromatography. J Chromatogr B Analyt Technol Biomed Life Sci. 2017 Mar 1;1046:211-217. PubMed PMID: 28199916.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isolation of four phenolic compounds from Mangifera indica L. flowers by using normal phase combined with elution extrusion two-step high speed countercurrent chromatography. AU - Shaheen,Nusrat, AU - Lu,Yanzhen, AU - Geng,Ping, AU - Shao,Qian, AU - Wei,Yun, Y1 - 2017/01/15/ PY - 2016/10/29/received PY - 2016/12/08/revised PY - 2017/01/14/accepted PY - 2017/2/16/pubmed PY - 2017/5/23/medline PY - 2017/2/16/entrez KW - (1)HNMR KW - (13)C NMR KW - HSCCC KW - Mangifera indica L. flowers KW - Phenolic compounds KW - UPLC-QTOF/ESI–MS SP - 211 EP - 217 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 1046 N2 - Two-step high speed countercurrent chromatography method, following normal phase and elution-extrusion mode of operation by using selected solvent systems, was introduced for phenolic compounds separation. Phenolic compounds including gallic acid, ethyl gallate, ethyl digallate and ellagic acid were separated from the ethanol extract of mango (Mangifera indica L.) flowers for the first time. In the first step, gallic acid of 3.7mg and ethyl gallate of 3.9mg with the purities of 98.87% and 99.55%, respectively, were isolated by using hexane-ethylacetate-methanol-water (4:6:4:6, v/v) in normal phase high speed countercurrent chromatography from 200mg of crude extract, while ethyl digallate and ellagic acid were collected in the form of mixture fraction. In the second step, further purification of the mixture was carried out with the help of another selected solvent system of dichloromethane-methanol-water (4:3:2, v/v) following elusion-extrusion mode of operation. Ethyl digallate of 3.8mg and ellagic acid of 5.7mg were separated well with high purities of 98.68% and 99.71%, respectively. The separated phenolic compounds were identified and confirmed by HPLC, UPLC-QTOF/ESI-MS, 1H and 13C NMR spectrometric analysis. SN - 1873-376X UR - https://www.unboundmedicine.com/medline/citation/28199916/Isolation_of_four_phenolic_compounds_from_Mangifera_indica_L__flowers_by_using_normal_phase_combined_with_elution_extrusion_two_step_high_speed_countercurrent_chromatography_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(16)30975-8 DB - PRIME DP - Unbound Medicine ER -