Tags

Type your tag names separated by a space and hit enter

Role of eicosanoids in hypoxic vasoconstriction in isolated lamb lungs.
Am J Physiol. 1987 Sep; 253(3 Pt 2):H626-33.AJ

Abstract

To determine the role of eicosanoids in hypoxic pulmonary vasoconstriction, we studied 42 isolated, blood-perfused lamb lungs during normoxia and hypoxia. We used the lung micropuncture technique to measure microvascular pressures in 20- to 80-micron diameter arterioles and venules and estimated segmental vascular resistance. In separate experiments, lungs were untreated or treated with either indomethacin (a cyclooxygenase inhibitor), Dazmegrel (a thromboxane synthetase inhibitor), SQ 29548 (a thromboxane receptor blocker), FPL 57231 (a leukotriene receptor blocker), or U 60257 (a 5'lipoxygenase inhibitor). In control untreated lungs both pulmonary arteries and veins constricted during hypoxia. Addition of indomethacin, Dazmegrel, or SQ 29548 to the perfusate resulted in abolition of venous constriction during hypoxia but enhancement of arterial constriction. FPL 57231 or U 60257 resulted in complete abolition of the pulmonary hypoxic vasoconstrictor response. Our results indicate that during hypoxia, leukotrienes mediate arterial and venous constriction with thromboxane A2 being necessary for venous constriction. We conclude that the interaction between 5'lipoxygenase and cyclooxygenase products of arachidonic acid results in the characteristic pulmonary hypoxic vasoconstrictor response in isolated, perfused lamb lungs.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

2820247

Citation

Raj, J U., and P Chen. "Role of Eicosanoids in Hypoxic Vasoconstriction in Isolated Lamb Lungs." The American Journal of Physiology, vol. 253, no. 3 Pt 2, 1987, pp. H626-33.
Raj JU, Chen P. Role of eicosanoids in hypoxic vasoconstriction in isolated lamb lungs. Am J Physiol. 1987;253(3 Pt 2):H626-33.
Raj, J. U., & Chen, P. (1987). Role of eicosanoids in hypoxic vasoconstriction in isolated lamb lungs. The American Journal of Physiology, 253(3 Pt 2), H626-33.
Raj JU, Chen P. Role of Eicosanoids in Hypoxic Vasoconstriction in Isolated Lamb Lungs. Am J Physiol. 1987;253(3 Pt 2):H626-33. PubMed PMID: 2820247.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Role of eicosanoids in hypoxic vasoconstriction in isolated lamb lungs. AU - Raj,J U, AU - Chen,P, PY - 1987/9/1/pubmed PY - 1987/9/1/medline PY - 1987/9/1/entrez SP - H626 EP - 33 JF - The American journal of physiology JO - Am J Physiol VL - 253 IS - 3 Pt 2 N2 - To determine the role of eicosanoids in hypoxic pulmonary vasoconstriction, we studied 42 isolated, blood-perfused lamb lungs during normoxia and hypoxia. We used the lung micropuncture technique to measure microvascular pressures in 20- to 80-micron diameter arterioles and venules and estimated segmental vascular resistance. In separate experiments, lungs were untreated or treated with either indomethacin (a cyclooxygenase inhibitor), Dazmegrel (a thromboxane synthetase inhibitor), SQ 29548 (a thromboxane receptor blocker), FPL 57231 (a leukotriene receptor blocker), or U 60257 (a 5'lipoxygenase inhibitor). In control untreated lungs both pulmonary arteries and veins constricted during hypoxia. Addition of indomethacin, Dazmegrel, or SQ 29548 to the perfusate resulted in abolition of venous constriction during hypoxia but enhancement of arterial constriction. FPL 57231 or U 60257 resulted in complete abolition of the pulmonary hypoxic vasoconstrictor response. Our results indicate that during hypoxia, leukotrienes mediate arterial and venous constriction with thromboxane A2 being necessary for venous constriction. We conclude that the interaction between 5'lipoxygenase and cyclooxygenase products of arachidonic acid results in the characteristic pulmonary hypoxic vasoconstrictor response in isolated, perfused lamb lungs. SN - 0002-9513 UR - https://www.unboundmedicine.com/medline/citation/2820247/Role_of_eicosanoids_in_hypoxic_vasoconstriction_in_isolated_lamb_lungs_ L2 - https://journals.physiology.org/doi/10.1152/ajpheart.1987.253.3.H626?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -