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The potentiating effect of calcitonin gene-related peptide on transient receptor potential vanilloid-1 activity and the electrophysiological responses of rat trigeminal neurons to nociceptive stimuli.
J Physiol Sci 2018; 68(3):261-268JP

Abstract

Growing evidence suggests that calcitonin gene-related peptide (CGRP) participates in trigeminal nociceptive responses. However, the role of CGRP in sensitization or desensitization of nociceptive transduction remains poorly understood. In this study, we sought to further investigate the CGRP-induced up-regulation of transient receptor potential vanilloid-1 (TRPV1) and the responses of trigeminal neurons to nociceptive stimuli. Rat trigeminal ganglion (TG) organ cultures and isolated trigeminal neurons were incubated with CGRP. An increase in TRPV1 levels was observed in CGRP-incubated TG organ cultures. CGRP potentiated capsaicin-induced increase in phosphorylated CaMKII levels in the TG organ cultures. The incubation of the trigeminal neurons with CGRP significantly increased the inward currents in response to capsaicin challenge, and this effect was inhibited by co-incubation with the CGRP receptor antagonist, BIBN4068BS or the inhibitor of protein kinase A, H-89. These findings reveal that CGRP acting on trigeminal neurons may play a significant role in facilitating cellular events that contribute to the peripheral sensitization of the TG in nociceptive transmission.

Authors+Show Affiliations

Research Center for Neuroscience, Institute of Molecular Biosciences, Mahidol University, Salaya, Nakhonpathom, 73170, Thailand.Australian School of Advanced Medicine, Macquarie University, Sydney, Australia.International Medical College, King Mongkut's Institute of Technology Ladkrabang, Bangkok, Thailand.Research Center for Neuroscience, Institute of Molecular Biosciences, Mahidol University, Salaya, Nakhonpathom, 73170, Thailand. banthit.che@mahidol.ac.th.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28205139

Citation

Chatchaisak, Duangthip, et al. "The Potentiating Effect of Calcitonin Gene-related Peptide On Transient Receptor Potential Vanilloid-1 Activity and the Electrophysiological Responses of Rat Trigeminal Neurons to Nociceptive Stimuli." The Journal of Physiological Sciences : JPS, vol. 68, no. 3, 2018, pp. 261-268.
Chatchaisak D, Connor M, Srikiatkhachorn A, et al. The potentiating effect of calcitonin gene-related peptide on transient receptor potential vanilloid-1 activity and the electrophysiological responses of rat trigeminal neurons to nociceptive stimuli. J Physiol Sci. 2018;68(3):261-268.
Chatchaisak, D., Connor, M., Srikiatkhachorn, A., & Chetsawang, B. (2018). The potentiating effect of calcitonin gene-related peptide on transient receptor potential vanilloid-1 activity and the electrophysiological responses of rat trigeminal neurons to nociceptive stimuli. The Journal of Physiological Sciences : JPS, 68(3), pp. 261-268. doi:10.1007/s12576-017-0529-9.
Chatchaisak D, et al. The Potentiating Effect of Calcitonin Gene-related Peptide On Transient Receptor Potential Vanilloid-1 Activity and the Electrophysiological Responses of Rat Trigeminal Neurons to Nociceptive Stimuli. J Physiol Sci. 2018;68(3):261-268. PubMed PMID: 28205139.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The potentiating effect of calcitonin gene-related peptide on transient receptor potential vanilloid-1 activity and the electrophysiological responses of rat trigeminal neurons to nociceptive stimuli. AU - Chatchaisak,Duangthip, AU - Connor,Mark, AU - Srikiatkhachorn,Anan, AU - Chetsawang,Banthit, Y1 - 2017/02/15/ PY - 2016/05/21/received PY - 2017/02/05/accepted PY - 2017/2/17/pubmed PY - 2018/9/8/medline PY - 2017/2/17/entrez KW - CGRP KW - Capsaicin KW - Nociceptive transmission KW - TRPV1 KW - Trigeminal ganglion SP - 261 EP - 268 JF - The journal of physiological sciences : JPS JO - J Physiol Sci VL - 68 IS - 3 N2 - Growing evidence suggests that calcitonin gene-related peptide (CGRP) participates in trigeminal nociceptive responses. However, the role of CGRP in sensitization or desensitization of nociceptive transduction remains poorly understood. In this study, we sought to further investigate the CGRP-induced up-regulation of transient receptor potential vanilloid-1 (TRPV1) and the responses of trigeminal neurons to nociceptive stimuli. Rat trigeminal ganglion (TG) organ cultures and isolated trigeminal neurons were incubated with CGRP. An increase in TRPV1 levels was observed in CGRP-incubated TG organ cultures. CGRP potentiated capsaicin-induced increase in phosphorylated CaMKII levels in the TG organ cultures. The incubation of the trigeminal neurons with CGRP significantly increased the inward currents in response to capsaicin challenge, and this effect was inhibited by co-incubation with the CGRP receptor antagonist, BIBN4068BS or the inhibitor of protein kinase A, H-89. These findings reveal that CGRP acting on trigeminal neurons may play a significant role in facilitating cellular events that contribute to the peripheral sensitization of the TG in nociceptive transmission. SN - 1880-6562 UR - https://www.unboundmedicine.com/medline/citation/28205139/The_potentiating_effect_of_calcitonin_gene_related_peptide_on_transient_receptor_potential_vanilloid_1_activity_and_the_electrophysiological_responses_of_rat_trigeminal_neurons_to_nociceptive_stimuli_ L2 - https://dx.doi.org/10.1007/s12576-017-0529-9 DB - PRIME DP - Unbound Medicine ER -