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Influence of nutrient signals and carbon allocation on the expression of phosphate and nitrogen transporter genes in winter wheat (Triticum aestivum L.) roots colonized by arbuscular mycorrhizal fungi.
PLoS One. 2017; 12(2):e0172154.Plos

Abstract

Arbuscular mycorrhizal (AM) colonization of plant roots causes the down-regulation of expression of phosphate (Pi) or nitrogen (N) transporter genes involved in direct nutrient uptake pathways. The mechanism of this effect remains unknown. In the present study, we sought to determine whether the expression of Pi or N transporter genes in roots of winter wheat colonized by AM fungus responded to (1) Pi or N nutrient signals transferred from the AM extra-radical hyphae, or (2) carbon allocation changes in the AM association. A three-compartment culture system, comprising a root compartment (RC), a root and AM hyphae compartment (RHC), and an AM hyphae compartment (HC), was used to test whether the expression of Pi or N transporter genes responded to nutrients (Pi, NH4+ and NO3-) added only to the HC. Different AM inoculation density treatments (roots were inoculated with 0, 20, 50 and 200 g AM inoculum) and light regime treatments (6 hours light and 18 hours light) were established to test the effects of carbon allocation on the expression of Pi or N transporter genes in wheat roots. The expression of two Pi transporter genes (TaPT4 and TaPHT1.2), five nitrate transporter genes (TaNRT1.1, TaNRT1.2, TaNRT2.1, TaNRT2.2, and TaNRT2.3), and an ammonium transporter gene (TaAMT1.2) was quantified using real-time polymerase chain reaction. The expression of TaPT4, TaNRT2.2, and TaAMT1.2 was down-regulated by AM colonization only when roots of host plants received Pi or N nutrient signals. However, the expression of TaPHT1.2, TaNRT2.1, and TaNRT2.3 was down-regulated by AM colonization, regardless of whether there was nutrient transfer from AM hyphae. The expression of TaNRT1.2 was also down-regulated by AM colonization even when there was no nutrient transfer from AM hyphae. The present study showed that an increase in carbon consumption by the AM fungi did not necessarily result in greater down-regulation of expression of Pi or N transporter genes.

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Authors+Show Affiliations

Key Laboratory of Plant Nutrition and Agri-environment in Northwest China, Ministry of Agriculture, College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi, China.

Key Laboratory of Plant Nutrition and Agri-environment in Northwest China, Ministry of Agriculture, College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi, China.

Key Laboratory of Plant Nutrition and Agri-environment in Northwest China, Ministry of Agriculture, College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi, China.

Key Laboratory of Plant Nutrition and Agri-environment in Northwest China, Ministry of Agriculture, College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi, China.

Key Laboratory of Plant Nutrition and Agri-environment in Northwest China, Ministry of Agriculture, College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi, China.

Key Laboratory of Plant Nutrition and Agri-environment in Northwest China, Ministry of Agriculture, College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi, China.

MeSH

CarbonFoodGene Expression Regulation, PlantMycorrhizaeNitrogenPhosphate Transport ProteinsPhosphatesPlant ProteinsPlant RootsSymbiosisTriticum

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28207830

Citation

Tian, Hui, et al. "Influence of Nutrient Signals and Carbon Allocation On the Expression of Phosphate and Nitrogen Transporter Genes in Winter Wheat (Triticum Aestivum L.) Roots Colonized By Arbuscular Mycorrhizal Fungi." PloS One, vol. 12, no. 2, 2017, pp. e0172154.

Tian H, Yuan X, Duan J, et al. Influence of nutrient signals and carbon allocation on the expression of phosphate and nitrogen transporter genes in winter wheat (Triticum aestivum L.) roots colonized by arbuscular mycorrhizal fungi. PLoS One. 2017;12(2):e0172154.

Tian, H., Yuan, X., Duan, J., Li, W., Zhai, B., & Gao, Y. (2017). Influence of nutrient signals and carbon allocation on the expression of phosphate and nitrogen transporter genes in winter wheat (Triticum aestivum L.) roots colonized by arbuscular mycorrhizal fungi. PloS One, 12(2), e0172154. https://doi.org/10.1371/journal.pone.0172154

Tian H, et al. Influence of Nutrient Signals and Carbon Allocation On the Expression of Phosphate and Nitrogen Transporter Genes in Winter Wheat (Triticum Aestivum L.) Roots Colonized By Arbuscular Mycorrhizal Fungi. PLoS One. 2017;12(2):e0172154. PubMed PMID: 28207830.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Influence of nutrient signals and carbon allocation on the expression of phosphate and nitrogen transporter genes in winter wheat (Triticum aestivum L.) roots colonized by arbuscular mycorrhizal fungi. AU - Tian,Hui, AU - Yuan,Xiaolei, AU - Duan,Jianfeng, AU - Li,Wenhu, AU - Zhai,Bingnian, AU - Gao,Yajun, Y1 - 2017/02/16/ PY - 2016/10/24/received PY - 2017/01/31/accepted PY - 2017/2/17/entrez PY - 2017/2/17/pubmed PY - 2017/8/29/medline SP - e0172154 EP - e0172154 JF - PloS one JO - PLoS One VL - 12 IS - 2 N2 - Arbuscular mycorrhizal (AM) colonization of plant roots causes the down-regulation of expression of phosphate (Pi) or nitrogen (N) transporter genes involved in direct nutrient uptake pathways. The mechanism of this effect remains unknown. In the present study, we sought to determine whether the expression of Pi or N transporter genes in roots of winter wheat colonized by AM fungus responded to (1) Pi or N nutrient signals transferred from the AM extra-radical hyphae, or (2) carbon allocation changes in the AM association. A three-compartment culture system, comprising a root compartment (RC), a root and AM hyphae compartment (RHC), and an AM hyphae compartment (HC), was used to test whether the expression of Pi or N transporter genes responded to nutrients (Pi, NH4+ and NO3-) added only to the HC. Different AM inoculation density treatments (roots were inoculated with 0, 20, 50 and 200 g AM inoculum) and light regime treatments (6 hours light and 18 hours light) were established to test the effects of carbon allocation on the expression of Pi or N transporter genes in wheat roots. The expression of two Pi transporter genes (TaPT4 and TaPHT1.2), five nitrate transporter genes (TaNRT1.1, TaNRT1.2, TaNRT2.1, TaNRT2.2, and TaNRT2.3), and an ammonium transporter gene (TaAMT1.2) was quantified using real-time polymerase chain reaction. The expression of TaPT4, TaNRT2.2, and TaAMT1.2 was down-regulated by AM colonization only when roots of host plants received Pi or N nutrient signals. However, the expression of TaPHT1.2, TaNRT2.1, and TaNRT2.3 was down-regulated by AM colonization, regardless of whether there was nutrient transfer from AM hyphae. The expression of TaNRT1.2 was also down-regulated by AM colonization even when there was no nutrient transfer from AM hyphae. The present study showed that an increase in carbon consumption by the AM fungi did not necessarily result in greater down-regulation of expression of Pi or N transporter genes. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/28207830/Influence_of_nutrient_signals_and_carbon_allocation_on_the_expression_of_phosphate_and_nitrogen_transporter_genes_in_winter_wheat__Triticum_aestivum_L___roots_colonized_by_arbuscular_mycorrhizal_fungi_ L2 - https://dx.plos.org/10.1371/journal.pone.0172154 DB - PRIME DP - Unbound Medicine ER -