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Monitoring of macrophage accumulation in statin-treated atherosclerotic mouse model using sodium iodide symporter imaging system.
Nucl Med Biol 2017; 48:45-51NM

Abstract

INTRODUCTION

Macrophages play a key role in atherosclerotic plaque formation in atherosclerosis, but its detailed understanding has poorly investigated until now. Thus, we sought to demonstrate a noninvasive technique for macrophage tracking to atherosclerotic lesions in apolipoprotein E-/-(ApoE-/-) mice with an imaging system based on sodium iodide symporter (NIS) gene coupled with 99mTc-single-photon emission computed tomography (SPECT).

METHODS AND RESULTS

Macrophage cells (RAW264.7) were stably transduced with retrovirus expressing NIS gene (RAW-NIS). In RAW-NIS cells, uptake of 125I was higher than the parental cells. [18F]FDG signals in the aorta at 30weeks on an ApoE-/- mice with high cholesterol diet were higher (1.7±0.12% injected dose (ID)) than those in control group (0.84±0.06% ID). Through 99mTc-SPECT/computed tomography (CT), in the RAW-NIS cell injected group, the 99mTc-pertechnetate uptake in aorta was higher than control groups. However, according to atorvastatin treatment, RAW-NIS cell recruitment reduced to the aorta. Area of 99mTc-pertechnetate uptake was positively correlated with immunostaining results against macrophage antigen (CD68). Cholesterol and low-density lipoprotein levels of atorvastatin-treated group showed lower than those of atorvastatin-untreated group, but did not reach statistical difference.

CONCLUSIONS

This novel approach to tracking macrophages to atherosclerotic plaques in vivo can be applied for studies of arterosclerotic vascular disease.

Authors+Show Affiliations

Division of RI-Convergence Research, Korea Institute of Radiological & Medical Sciences, Seoul, Republic of Korea; Department of Laboratory Animal Medicine, College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea.Division of RI-Convergence Research, Korea Institute of Radiological & Medical Sciences, Seoul, Republic of Korea.Division of RI-Convergence Research, Korea Institute of Radiological & Medical Sciences, Seoul, Republic of Korea.Division of RI-Convergence Research, Korea Institute of Radiological & Medical Sciences, Seoul, Republic of Korea.Division of RI-Convergence Research, Korea Institute of Radiological & Medical Sciences, Seoul, Republic of Korea.Department of Laboratory Animal Medicine, College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea.Division of RI-Convergence Research, Korea Institute of Radiological & Medical Sciences, Seoul, Republic of Korea.Department of Nuclear Medicine, Korea Institute of Radiological & Medical Sciences, Seoul, Republic of Korea.Division of RI-Convergence Research, Korea Institute of Radiological & Medical Sciences, Seoul, Republic of Korea. Electronic address: yjlee@kirams.re.kr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28208058

Citation

Yoo, Ran Ji, et al. "Monitoring of Macrophage Accumulation in Statin-treated Atherosclerotic Mouse Model Using Sodium Iodide Symporter Imaging System." Nuclear Medicine and Biology, vol. 48, 2017, pp. 45-51.
Yoo RJ, Kim MH, Woo SK, et al. Monitoring of macrophage accumulation in statin-treated atherosclerotic mouse model using sodium iodide symporter imaging system. Nucl Med Biol. 2017;48:45-51.
Yoo, R. J., Kim, M. H., Woo, S. K., Kim, K. I., Lee, T. S., Choi, Y. K., ... Lee, Y. J. (2017). Monitoring of macrophage accumulation in statin-treated atherosclerotic mouse model using sodium iodide symporter imaging system. Nuclear Medicine and Biology, 48, pp. 45-51. doi:10.1016/j.nucmedbio.2017.01.009.
Yoo RJ, et al. Monitoring of Macrophage Accumulation in Statin-treated Atherosclerotic Mouse Model Using Sodium Iodide Symporter Imaging System. Nucl Med Biol. 2017;48:45-51. PubMed PMID: 28208058.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Monitoring of macrophage accumulation in statin-treated atherosclerotic mouse model using sodium iodide symporter imaging system. AU - Yoo,Ran Ji, AU - Kim,Min Hwan, AU - Woo,Sang-Keun, AU - Kim,Kwang Il, AU - Lee,Tae Sup, AU - Choi,Yang-Kyu, AU - Kang,Joo Hyun, AU - Lim,Sang Moo, AU - Lee,Yong Jin, Y1 - 2017/01/25/ PY - 2016/12/27/received PY - 2017/01/16/revised PY - 2017/01/20/accepted PY - 2017/2/17/pubmed PY - 2017/11/29/medline PY - 2017/2/17/entrez KW - Atherosclerosis KW - Atorvastatin treatment KW - Macrophages KW - Reporter gene KW - SPECT imaging SP - 45 EP - 51 JF - Nuclear medicine and biology JO - Nucl. Med. Biol. VL - 48 N2 - INTRODUCTION: Macrophages play a key role in atherosclerotic plaque formation in atherosclerosis, but its detailed understanding has poorly investigated until now. Thus, we sought to demonstrate a noninvasive technique for macrophage tracking to atherosclerotic lesions in apolipoprotein E-/-(ApoE-/-) mice with an imaging system based on sodium iodide symporter (NIS) gene coupled with 99mTc-single-photon emission computed tomography (SPECT). METHODS AND RESULTS: Macrophage cells (RAW264.7) were stably transduced with retrovirus expressing NIS gene (RAW-NIS). In RAW-NIS cells, uptake of 125I was higher than the parental cells. [18F]FDG signals in the aorta at 30weeks on an ApoE-/- mice with high cholesterol diet were higher (1.7±0.12% injected dose (ID)) than those in control group (0.84±0.06% ID). Through 99mTc-SPECT/computed tomography (CT), in the RAW-NIS cell injected group, the 99mTc-pertechnetate uptake in aorta was higher than control groups. However, according to atorvastatin treatment, RAW-NIS cell recruitment reduced to the aorta. Area of 99mTc-pertechnetate uptake was positively correlated with immunostaining results against macrophage antigen (CD68). Cholesterol and low-density lipoprotein levels of atorvastatin-treated group showed lower than those of atorvastatin-untreated group, but did not reach statistical difference. CONCLUSIONS: This novel approach to tracking macrophages to atherosclerotic plaques in vivo can be applied for studies of arterosclerotic vascular disease. SN - 1872-9614 UR - https://www.unboundmedicine.com/medline/citation/28208058/Monitoring_of_macrophage_accumulation_in_statin_treated_atherosclerotic_mouse_model_using_sodium_iodide_symporter_imaging_system_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0969-8051(16)30445-0 DB - PRIME DP - Unbound Medicine ER -