Tags

Type your tag names separated by a space and hit enter

Nuclear localization of a putative Phytophthora sojae bZIP1 transcription factor is mediated by multiple targeting motifs.
Mol Microbiol. 2017 05; 104(4):621-635.MM

Abstract

Oomycetes are fungal-like eukaryotic microbes in the kingdom Stramenopila. We recently found that the oomycete plant pathogen Phytophthora sojae uses nuclear localization signals (NLSs) for translocation of proteins into the nucleus that differ from conventional well-characterized NLSs from mammals and yeast. Here, we have characterized in depth the NLSs of a P. sojae basic leucine zipper transcription factor, PsbZIP1. Nuclear localization of PsbZIP1 was determined by a central conserved region overlapping the DNA binding domain. Mutational analysis of this region identified four distinct elements that contributed multiplicatively to nuclear localization, but the conserved DNA binding residues were not required. Three of the elements showed autonomous NLS activity and the fourth served as a nuclear localization enhancer. Sequences within two of the nuclear localization elements defined a new form of bipartite NLS consisting of a triplet of basic residues followed by a tail of scattered basic amino acids.

Authors+Show Affiliations

Interdisciplinary Ph.D. Program in Genetics, Bioinformatics & Computational Biology, Virginia Polytechnic Institute and State University (Virginia Tech), Blacksburg, VA, 24061, USA. Center for Genome Research and Biocomputing and Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR, 97331, USA.Interdisciplinary Ph.D. Program in Genetics, Bioinformatics & Computational Biology, Virginia Polytechnic Institute and State University (Virginia Tech), Blacksburg, VA, 24061, USA. Center for Genome Research and Biocomputing and Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR, 97331, USA.

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

28213898

Citation

Fang, Yufeng, and Brett M. Tyler. "Nuclear Localization of a Putative Phytophthora Sojae bZIP1 Transcription Factor Is Mediated By Multiple Targeting Motifs." Molecular Microbiology, vol. 104, no. 4, 2017, pp. 621-635.
Fang Y, Tyler BM. Nuclear localization of a putative Phytophthora sojae bZIP1 transcription factor is mediated by multiple targeting motifs. Mol Microbiol. 2017;104(4):621-635.
Fang, Y., & Tyler, B. M. (2017). Nuclear localization of a putative Phytophthora sojae bZIP1 transcription factor is mediated by multiple targeting motifs. Molecular Microbiology, 104(4), 621-635. https://doi.org/10.1111/mmi.13652
Fang Y, Tyler BM. Nuclear Localization of a Putative Phytophthora Sojae bZIP1 Transcription Factor Is Mediated By Multiple Targeting Motifs. Mol Microbiol. 2017;104(4):621-635. PubMed PMID: 28213898.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nuclear localization of a putative Phytophthora sojae bZIP1 transcription factor is mediated by multiple targeting motifs. AU - Fang,Yufeng, AU - Tyler,Brett M, Y1 - 2017/03/16/ PY - 2017/02/14/accepted PY - 2017/2/19/pubmed PY - 2017/10/17/medline PY - 2017/2/19/entrez SP - 621 EP - 635 JF - Molecular microbiology JO - Mol. Microbiol. VL - 104 IS - 4 N2 - Oomycetes are fungal-like eukaryotic microbes in the kingdom Stramenopila. We recently found that the oomycete plant pathogen Phytophthora sojae uses nuclear localization signals (NLSs) for translocation of proteins into the nucleus that differ from conventional well-characterized NLSs from mammals and yeast. Here, we have characterized in depth the NLSs of a P. sojae basic leucine zipper transcription factor, PsbZIP1. Nuclear localization of PsbZIP1 was determined by a central conserved region overlapping the DNA binding domain. Mutational analysis of this region identified four distinct elements that contributed multiplicatively to nuclear localization, but the conserved DNA binding residues were not required. Three of the elements showed autonomous NLS activity and the fourth served as a nuclear localization enhancer. Sequences within two of the nuclear localization elements defined a new form of bipartite NLS consisting of a triplet of basic residues followed by a tail of scattered basic amino acids. SN - 1365-2958 UR - https://www.unboundmedicine.com/medline/citation/28213898/Nuclear_localization_of_a_putative_Phytophthora_sojae_bZIP1_transcription_factor_is_mediated_by_multiple_targeting_motifs_ L2 - https://doi.org/10.1111/mmi.13652 DB - PRIME DP - Unbound Medicine ER -