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Changes in IL-4 and IL-13 expression in allergic-rhinitis treated with hydrogen-rich saline in guinea-pig model.
Allergol Immunopathol (Madr). 2017 Jul - Aug; 45(4):350-355.AI

Abstract

BACKGROUND

Medical gas hydrogen (H2) has a special role in airway inflammation; however, the effect of H2 on allergic rhinitis (AR) remains unclear. This study explored the possible roles of H2 on the pathogenesis of AR and observed the influences of H2 on cytokines IL-4 and IL-13.

METHODS

An AR guinea pig model was established by nasal ovalbumin sensitisation. Eighteen guinea pigs were divided into three groups, namely, saline control, AR-sensitised, and hydrogen-rich saline (HRS)-treated groups, with each group having six guinea pigs. The frequencies of sneezing and scratching were recorded. The IgE level and cytokine (IL-4 and IL-13) levels in the serum were measured. The expression levels of IL-4 and IL-13 mRNA and protein in the nasal mucosa were also determined by real-time reverse transcriptase-polymerase chain reaction and Western blot. We also observed the infiltration of cytokine (IL-4 and IL-13) in nasal mucosa by immunofluorescence.

RESULTS

The frequencies of sneezing and scratching, as well as the levels of IgE, IL-4, and IL-13, in the serum were higher in the AR group than in the control group (p<0.01), whereas all these parameters were decreased significantly after HRS treatment (p<0.05). The expression levels of IL-4 and IL-13 mRNA and protein in the nasal mucosa were also lower in guinea pigs treated with HRS than those in the AR group (p<0.05).

CONCLUSIONS

HRS could affect anti-inflammation in AR and decreased the expression of IL-4 and IL-13.

Authors+Show Affiliations

Department of Otolaryngology, Tongji Hospital, Tongji University, Shanghai 200065, China.Department of Otolaryngology, Tongji Hospital, Tongji University, Shanghai 200065, China. Electronic address: yu_shaoqing@163.com.Department of Otolaryngology, Tongji Hospital, Tongji University, Shanghai 200065, China.Department of Otolaryngology, Tongji Hospital, Tongji University, Shanghai 200065, China.Department of Otolaryngology, Tongji Hospital, Tongji University, Shanghai 200065, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28215576

Citation

Zhao, C, et al. "Changes in IL-4 and IL-13 Expression in Allergic-rhinitis Treated With Hydrogen-rich Saline in Guinea-pig Model." Allergologia Et Immunopathologia, vol. 45, no. 4, 2017, pp. 350-355.
Zhao C, Yu S, Li J, et al. Changes in IL-4 and IL-13 expression in allergic-rhinitis treated with hydrogen-rich saline in guinea-pig model. Allergol Immunopathol (Madr). 2017;45(4):350-355.
Zhao, C., Yu, S., Li, J., Xu, W., & Ge, R. (2017). Changes in IL-4 and IL-13 expression in allergic-rhinitis treated with hydrogen-rich saline in guinea-pig model. Allergologia Et Immunopathologia, 45(4), 350-355. https://doi.org/10.1016/j.aller.2016.10.007
Zhao C, et al. Changes in IL-4 and IL-13 Expression in Allergic-rhinitis Treated With Hydrogen-rich Saline in Guinea-pig Model. Allergol Immunopathol (Madr). 2017;45(4):350-355. PubMed PMID: 28215576.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Changes in IL-4 and IL-13 expression in allergic-rhinitis treated with hydrogen-rich saline in guinea-pig model. AU - Zhao,C, AU - Yu,S, AU - Li,J, AU - Xu,W, AU - Ge,R, Y1 - 2017/02/16/ PY - 2016/08/28/received PY - 2016/10/31/accepted PY - 2017/2/22/pubmed PY - 2018/4/7/medline PY - 2017/2/21/entrez KW - Allergic rhinitis KW - Hydrogen-rich saline KW - IgE KW - Immunofluorescence KW - Ovalbumin SP - 350 EP - 355 JF - Allergologia et immunopathologia JO - Allergol Immunopathol (Madr) VL - 45 IS - 4 N2 - BACKGROUND: Medical gas hydrogen (H2) has a special role in airway inflammation; however, the effect of H2 on allergic rhinitis (AR) remains unclear. This study explored the possible roles of H2 on the pathogenesis of AR and observed the influences of H2 on cytokines IL-4 and IL-13. METHODS: An AR guinea pig model was established by nasal ovalbumin sensitisation. Eighteen guinea pigs were divided into three groups, namely, saline control, AR-sensitised, and hydrogen-rich saline (HRS)-treated groups, with each group having six guinea pigs. The frequencies of sneezing and scratching were recorded. The IgE level and cytokine (IL-4 and IL-13) levels in the serum were measured. The expression levels of IL-4 and IL-13 mRNA and protein in the nasal mucosa were also determined by real-time reverse transcriptase-polymerase chain reaction and Western blot. We also observed the infiltration of cytokine (IL-4 and IL-13) in nasal mucosa by immunofluorescence. RESULTS: The frequencies of sneezing and scratching, as well as the levels of IgE, IL-4, and IL-13, in the serum were higher in the AR group than in the control group (p<0.01), whereas all these parameters were decreased significantly after HRS treatment (p<0.05). The expression levels of IL-4 and IL-13 mRNA and protein in the nasal mucosa were also lower in guinea pigs treated with HRS than those in the AR group (p<0.05). CONCLUSIONS: HRS could affect anti-inflammation in AR and decreased the expression of IL-4 and IL-13. SN - 1578-1267 UR - https://www.unboundmedicine.com/medline/citation/28215576/Changes_in_IL_4_and_IL_13_expression_in_allergic_rhinitis_treated_with_hydrogen_rich_saline_in_guinea_pig_model_ L2 - http://www.elsevier.es/en/linksolver/ft/pii/S0301-0546(16)30160-4 DB - PRIME DP - Unbound Medicine ER -