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Interleukin 10 inhibits pro-inflammatory cytokine responses and killing of Burkholderia pseudomallei.
Sci Rep. 2017 02 20; 7:42791.SR

Abstract

Melioidosis, caused by Burkholderia pseudomallei, is endemic in northeastern Thailand and Northern Australia. Severe septicemic melioidosis is associated with high levels of pro-inflammatory cytokines and is correlated with poor clinical outcomes. IL-10 is an immunoregulatory cytokine, which in other infections can control the expression of pro-inflammatory cytokines, but its role in melioidosis has not been addressed. Here, whole blood of healthy seropositive individuals (n = 75), living in N. E. Thailand was co-cultured with B. pseudomallei and production of IL-10 and IFN-γ detected and the cellular sources identified. CD3- CD14+ monocytes were the main source of IL-10. Neutralization of IL-10 increased IFN-γ, IL-6 and TNF-α production and improved bacteria killing. IFN-γ production and microbicidal activity were impaired in individuals with diabetes mellitus (DM). In contrast, IL-10 production was unimpaired in individuals with DM, resulting in an IL-10 dominant cytokine balance. Neutralization of IL-10 restored the IFN-γ response of individuals with DM to similar levels observed in healthy individuals and improved killing of B. pseudomallei in vitro. These results demonstrate that monocyte derived IL-10 acts to inhibit potentially protective cell mediated immune responses against B. pseudomallei, but may also moderate the pathological effects of excessive cytokine production during sepsis.

Authors+Show Affiliations

Centre for Research and Development of Medical Diagnostic Laboratories, Khon Kaen University, Khon Kaen, Thailand.Centre for Research and Development of Medical Diagnostic Laboratories, Khon Kaen University, Khon Kaen, Thailand.Centre for Research and Development of Medical Diagnostic Laboratories, Khon Kaen University, Khon Kaen, Thailand.Centre for Research and Development of Medical Diagnostic Laboratories, Khon Kaen University, Khon Kaen, Thailand.Department of Immunology and Infection, London School of Hygiene &Tropical Medicine, London, United Kingdom.Department of Asthma, Allergy and Respiratory Science, Guys Hospital, Kings College, London, United Kingdom.Department of Immunology and Infection, London School of Hygiene &Tropical Medicine, London, United Kingdom.Centre for Research and Development of Medical Diagnostic Laboratories, Khon Kaen University, Khon Kaen, Thailand.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

28216665

Citation

Kessler, Bianca, et al. "Interleukin 10 Inhibits Pro-inflammatory Cytokine Responses and Killing of Burkholderia Pseudomallei." Scientific Reports, vol. 7, 2017, p. 42791.
Kessler B, Rinchai D, Kewcharoenwong C, et al. Interleukin 10 inhibits pro-inflammatory cytokine responses and killing of Burkholderia pseudomallei. Sci Rep. 2017;7:42791.
Kessler, B., Rinchai, D., Kewcharoenwong, C., Nithichanon, A., Biggart, R., Hawrylowicz, C. M., Bancroft, G. J., & Lertmemongkolchai, G. (2017). Interleukin 10 inhibits pro-inflammatory cytokine responses and killing of Burkholderia pseudomallei. Scientific Reports, 7, 42791. https://doi.org/10.1038/srep42791
Kessler B, et al. Interleukin 10 Inhibits Pro-inflammatory Cytokine Responses and Killing of Burkholderia Pseudomallei. Sci Rep. 2017 02 20;7:42791. PubMed PMID: 28216665.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Interleukin 10 inhibits pro-inflammatory cytokine responses and killing of Burkholderia pseudomallei. AU - Kessler,Bianca, AU - Rinchai,Darawan, AU - Kewcharoenwong,Chidchamai, AU - Nithichanon,Arnone, AU - Biggart,Rachael, AU - Hawrylowicz,Catherine M, AU - Bancroft,Gregory J, AU - Lertmemongkolchai,Ganjana, Y1 - 2017/02/20/ PY - 2016/06/30/received PY - 2017/01/17/accepted PY - 2017/2/21/entrez PY - 2017/2/22/pubmed PY - 2018/10/30/medline SP - 42791 EP - 42791 JF - Scientific reports JO - Sci Rep VL - 7 N2 - Melioidosis, caused by Burkholderia pseudomallei, is endemic in northeastern Thailand and Northern Australia. Severe septicemic melioidosis is associated with high levels of pro-inflammatory cytokines and is correlated with poor clinical outcomes. IL-10 is an immunoregulatory cytokine, which in other infections can control the expression of pro-inflammatory cytokines, but its role in melioidosis has not been addressed. Here, whole blood of healthy seropositive individuals (n = 75), living in N. E. Thailand was co-cultured with B. pseudomallei and production of IL-10 and IFN-γ detected and the cellular sources identified. CD3- CD14+ monocytes were the main source of IL-10. Neutralization of IL-10 increased IFN-γ, IL-6 and TNF-α production and improved bacteria killing. IFN-γ production and microbicidal activity were impaired in individuals with diabetes mellitus (DM). In contrast, IL-10 production was unimpaired in individuals with DM, resulting in an IL-10 dominant cytokine balance. Neutralization of IL-10 restored the IFN-γ response of individuals with DM to similar levels observed in healthy individuals and improved killing of B. pseudomallei in vitro. These results demonstrate that monocyte derived IL-10 acts to inhibit potentially protective cell mediated immune responses against B. pseudomallei, but may also moderate the pathological effects of excessive cytokine production during sepsis. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/28216665/Interleukin_10_inhibits_pro_inflammatory_cytokine_responses_and_killing_of_Burkholderia_pseudomallei_ L2 - https://doi.org/10.1038/srep42791 DB - PRIME DP - Unbound Medicine ER -