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Ultrasound-Assisted Extraction and Identification of Natural Antioxidants from the Fruit of Melastoma sanguineum Sims.
Molecules. 2017 Feb 18; 22(2)M

Abstract

The fruit of Melastoma sanguineum Sims is an edible and sweet wild fruit. In our previous study, the fruit was found to have a strong antioxidant property. In this study, an ultrasound-assisted extraction (UAE) method was developed to extract natural antioxidants from the fruit of Melastoma sanguineum Sims, and a response surface methodology was used to optimize the conditions of UAE to maximize the extraction efficiency. The influence of five independent extraction parameters (ethanol concentration, solvent/material ratio, extracting time, temperature, and ultrasound power) on the extraction efficiency were investigated using a single factor experiment, and then a central composite rotatable design was used to investigate the interaction of three key parameters. The results showed that the optimal extraction conditions were 42.98% ethanol, 28.29 mL/g solvent/material ratio, 34.29 min extracting time, 60 °C temperature, and 600 W ultrasound power. Under these conditions, the Trolox equivalent antioxidant capacity (TEAC) value of the extracts was 1074.61 ± 32.56 μmol Trolox/g dry weight (DW). Compared with conventional maceration (723.27 ± 11.61 μmol Trolox/g DW) and Soxhlet extraction methods (518.37 ± 23.23 μmol Trolox/g DW), the UAE method improved the extraction efficiency, in a shorter period of time. In addition, epicatechin gallate, epicatechin, rutin, epigallocatechin, protocatechuic acid, chlorogenic acid, and quercetin, were identified and quantified in the fruit extracts of Melastoma sanguineum Sims by UPLC-MS/MS.

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Authors+Show Affiliations

Zhou T
Guangdong Provincial Key Laboratory of Food, Nutrition and Health, School of Public Health, Sun Yat-Sen University, Guangzhou 510080, China. zhout43@mail2.sysu.edu.cn.
Xu DP
Guangdong Provincial Key Laboratory of Food, Nutrition and Health, School of Public Health, Sun Yat-Sen University, Guangzhou 510080, China. xudp@mail2.sysu.edu.cn.
Lin SJ
Zhongshan Center for Disease Control and Prevention, Zhongshan 528403, China. zscdclsj@163.com.
Li Y
Guangdong Provincial Key Laboratory of Food, Nutrition and Health, School of Public Health, Sun Yat-Sen University, Guangzhou 510080, China. liya28@mail2.sysu.edu.cn.
Zheng J
Guangdong Provincial Key Laboratory of Food, Nutrition and Health, School of Public Health, Sun Yat-Sen University, Guangzhou 510080, China. zhengj37@mail2.sysu.edu.cn.
Zhou Y
Guangdong Provincial Key Laboratory of Food, Nutrition and Health, School of Public Health, Sun Yat-Sen University, Guangzhou 510080, China. zhouyue3@mail2.sysu.edu.cn.
Zhang JJ
Guangdong Provincial Key Laboratory of Food, Nutrition and Health, School of Public Health, Sun Yat-Sen University, Guangzhou 510080, China. zhangjj46@mail2.sysu.edu.cn.
Li HB
Guangdong Provincial Key Laboratory of Food, Nutrition and Health, School of Public Health, Sun Yat-Sen University, Guangzhou 510080, China. lihuabin@mail.sysu.edu.cn. South China Sea Bioresource Exploitation and Utilization Collaborative Innovation Center, Sun Yat-Sen University, Guangzhou 510006, China. lihuabin@mail.sysu.edu.cn.

MeSH

AntioxidantsChemical FractionationFruitMelastomataceaePhenolsPlant ExtractsSolventsUltrasonic Waves

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28218717

Citation

Zhou, Tong, et al. "Ultrasound-Assisted Extraction and Identification of Natural Antioxidants From the Fruit of Melastoma Sanguineum Sims." Molecules (Basel, Switzerland), vol. 22, no. 2, 2017.
Zhou T, Xu DP, Lin SJ, et al. Ultrasound-Assisted Extraction and Identification of Natural Antioxidants from the Fruit of Melastoma sanguineum Sims. Molecules. 2017;22(2).
Zhou, T., Xu, D. P., Lin, S. J., Li, Y., Zheng, J., Zhou, Y., Zhang, J. J., & Li, H. B. (2017). Ultrasound-Assisted Extraction and Identification of Natural Antioxidants from the Fruit of Melastoma sanguineum Sims. Molecules (Basel, Switzerland), 22(2). https://doi.org/10.3390/molecules22020306
Zhou T, et al. Ultrasound-Assisted Extraction and Identification of Natural Antioxidants From the Fruit of Melastoma Sanguineum Sims. Molecules. 2017 Feb 18;22(2) PubMed PMID: 28218717.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ultrasound-Assisted Extraction and Identification of Natural Antioxidants from the Fruit of Melastoma sanguineum Sims. AU - Zhou,Tong, AU - Xu,Dong-Ping, AU - Lin,Sheng-Jun, AU - Li,Ya, AU - Zheng,Jie, AU - Zhou,Yue, AU - Zhang,Jiao-Jiao, AU - Li,Hua-Bin, Y1 - 2017/02/18/ PY - 2017/01/13/received PY - 2017/02/10/revised PY - 2017/02/15/accepted PY - 2017/2/21/entrez PY - 2017/2/22/pubmed PY - 2017/5/10/medline KW - Melastoma sanguineum Sims KW - antioxidant KW - fruit KW - phenolic compounds KW - response surface methodology KW - ultrasound-assisted extraction JF - Molecules (Basel, Switzerland) JO - Molecules VL - 22 IS - 2 N2 - The fruit of Melastoma sanguineum Sims is an edible and sweet wild fruit. In our previous study, the fruit was found to have a strong antioxidant property. In this study, an ultrasound-assisted extraction (UAE) method was developed to extract natural antioxidants from the fruit of Melastoma sanguineum Sims, and a response surface methodology was used to optimize the conditions of UAE to maximize the extraction efficiency. The influence of five independent extraction parameters (ethanol concentration, solvent/material ratio, extracting time, temperature, and ultrasound power) on the extraction efficiency were investigated using a single factor experiment, and then a central composite rotatable design was used to investigate the interaction of three key parameters. The results showed that the optimal extraction conditions were 42.98% ethanol, 28.29 mL/g solvent/material ratio, 34.29 min extracting time, 60 °C temperature, and 600 W ultrasound power. Under these conditions, the Trolox equivalent antioxidant capacity (TEAC) value of the extracts was 1074.61 ± 32.56 μmol Trolox/g dry weight (DW). Compared with conventional maceration (723.27 ± 11.61 μmol Trolox/g DW) and Soxhlet extraction methods (518.37 ± 23.23 μmol Trolox/g DW), the UAE method improved the extraction efficiency, in a shorter period of time. In addition, epicatechin gallate, epicatechin, rutin, epigallocatechin, protocatechuic acid, chlorogenic acid, and quercetin, were identified and quantified in the fruit extracts of Melastoma sanguineum Sims by UPLC-MS/MS. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/28218717/Ultrasound_Assisted_Extraction_and_Identification_of_Natural_Antioxidants_from_the_Fruit_of_Melastoma_sanguineum_Sims_ L2 - https://www.mdpi.com/resolver?pii=molecules22020306 DB - PRIME DP - Unbound Medicine ER -