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Acquisition by a Campylobacter-like strain of aphA-1, a kanamycin resistance determinant from members of the family Enterobacteriaceae.
Antimicrob Agents Chemother 1987; 31(7):1021-6AA

Abstract

A Campylobacter-like organism, BM2196, resistant to kanamycin and streptomycin-spectinomycin was isolated from the feces of a patient with acute enteritis. The kanamycin and streptomycin-spectinomycin resistances were not transferable to Camplylobacter sp. or to Escherichia coli, and no plasmid DNA was detected in this strain. The resistance genes were therefore tentatively assigned to a chromosomal locality. Analysis by the phosphocellulose paper-binding assay of extracts from BM2196 indicated that resistance to kanamycin and structurally related antibiotics was due to the synthesis of 3'-aminoglycoside phosphotransferase type I [APH(3')-I], an enzyme specific for gram-negative bacteria, and that resistance to streptomycin-spectinomycin was secondary to the presence of a 3",9-aminoglycoside adenylyltransferase. Homology between BM2196 and an APH(3')-I probe was detected by DNA-DNA hybridization. A 2.2-kilobase BM2196 DNA fragment conferring resistance to kanamycin was cloned in E. coli and was sequenced partially. The resistance gene appeared nearly identical to that of Tn903 from E. coli and was adjacent to IS15-delta, an insertion sequence widespread in gram-negative bacteria, thus indicating that Campylobacter species can act as a recipient for genes originating in members of the family Enterobacteriaceae.

Authors+Show Affiliations

Unité des Agents Antibactériens, Centre National de la Recherche Scientifique, Institut Pasteur, France.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

2821885

Citation

Ouellette, M, et al. "Acquisition By a Campylobacter-like Strain of aphA-1, a Kanamycin Resistance Determinant From Members of the Family Enterobacteriaceae." Antimicrobial Agents and Chemotherapy, vol. 31, no. 7, 1987, pp. 1021-6.
Ouellette M, Gerbaud G, Lambert T, et al. Acquisition by a Campylobacter-like strain of aphA-1, a kanamycin resistance determinant from members of the family Enterobacteriaceae. Antimicrob Agents Chemother. 1987;31(7):1021-6.
Ouellette, M., Gerbaud, G., Lambert, T., & Courvalin, P. (1987). Acquisition by a Campylobacter-like strain of aphA-1, a kanamycin resistance determinant from members of the family Enterobacteriaceae. Antimicrobial Agents and Chemotherapy, 31(7), pp. 1021-6.
Ouellette M, et al. Acquisition By a Campylobacter-like Strain of aphA-1, a Kanamycin Resistance Determinant From Members of the Family Enterobacteriaceae. Antimicrob Agents Chemother. 1987;31(7):1021-6. PubMed PMID: 2821885.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Acquisition by a Campylobacter-like strain of aphA-1, a kanamycin resistance determinant from members of the family Enterobacteriaceae. AU - Ouellette,M, AU - Gerbaud,G, AU - Lambert,T, AU - Courvalin,P, PY - 1987/7/1/pubmed PY - 1987/7/1/medline PY - 1987/7/1/entrez SP - 1021 EP - 6 JF - Antimicrobial agents and chemotherapy JO - Antimicrob. Agents Chemother. VL - 31 IS - 7 N2 - A Campylobacter-like organism, BM2196, resistant to kanamycin and streptomycin-spectinomycin was isolated from the feces of a patient with acute enteritis. The kanamycin and streptomycin-spectinomycin resistances were not transferable to Camplylobacter sp. or to Escherichia coli, and no plasmid DNA was detected in this strain. The resistance genes were therefore tentatively assigned to a chromosomal locality. Analysis by the phosphocellulose paper-binding assay of extracts from BM2196 indicated that resistance to kanamycin and structurally related antibiotics was due to the synthesis of 3'-aminoglycoside phosphotransferase type I [APH(3')-I], an enzyme specific for gram-negative bacteria, and that resistance to streptomycin-spectinomycin was secondary to the presence of a 3",9-aminoglycoside adenylyltransferase. Homology between BM2196 and an APH(3')-I probe was detected by DNA-DNA hybridization. A 2.2-kilobase BM2196 DNA fragment conferring resistance to kanamycin was cloned in E. coli and was sequenced partially. The resistance gene appeared nearly identical to that of Tn903 from E. coli and was adjacent to IS15-delta, an insertion sequence widespread in gram-negative bacteria, thus indicating that Campylobacter species can act as a recipient for genes originating in members of the family Enterobacteriaceae. SN - 0066-4804 UR - https://www.unboundmedicine.com/medline/citation/2821885/Acquisition_by_a_Campylobacter_like_strain_of_aphA_1_a_kanamycin_resistance_determinant_from_members_of_the_family_Enterobacteriaceae_ L2 - http://aac.asm.org/cgi/pmidlookup?view=long&pmid=2821885 DB - PRIME DP - Unbound Medicine ER -