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Phytochemical screening and evaluation of in vitro antioxidant and antimicrobial activities of the indigenous medicinal plant Albizia odoratissima.
Pharm Biol. 2017 Dec; 55(1):1155-1161.PB

Abstract

CONTEXT

Albizia odoratissima (L. f.) Benth has been used in Indian folk medicine to treat numerous inflammatory pathologies, such as leprosy, ulcers, burns and asthma.

OBJECTIVE

To evaluate the antioxidant and antimicrobial properties of A. odoratissima.

MATERIALS AND METHODS

Dried leaves of A. odoratissima were extracted in organic solvents (hexane, chloroform, ethyl acetate, and methanol). The total phenolic content (TPC) and total flavonoid content (TFC) were determined using the Folin-Ciocalteu and aluminum chloride colorimetric methods, respectively. The antioxidant activity was examined using 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydrogen peroxide (H2O2), 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS), and ferric reducing antioxidant power (FRAP) assays. The antibacterial activity was examined using minimum inhibitory concentration (MIC) and the minimum bacterial concentration (MBC), determined by broth microdilution method against Gram-negative bacteria (Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa, and Proteus vulgaris) and Gram-positive bacterium (Staphylococcus aureus).

RESULTS

The TPC ranged from 4.40 ± 1.06 to 1166.66 ± 31.85 mg GAE/g of dry weight (DW), and the TFC ranged from 48.35 ± 3.62 to 109.74 ± 1.84 mg QE/g of DW. The IC50 values of the ethyl acetate extract for DPPH, ABTS, and H2O2 were 10.96 ± 0.40, 4.35 ± 0.07, and 163.82 ± 1.52 μg/mL, respectively. Both methanol and ethyl acetate extracts demonstrated effective antibacterial activity with MICs and MBCs values ranging 136-546 μg/mL and 273-1093 μg/mL, respectively, against the tested pathogenic species.

CONCLUSIONS

The leaves of A. odoratissima showed potent free radical scavenging property and antimicrobial activity.

Authors+Show Affiliations

a Department of Bio-Technology , Center for Bio-Technology, Institute of Science & Technology, Jawaharlal Nehru Technological University Hyderabad , Hyderabad , Telangana , India.a Department of Bio-Technology , Center for Bio-Technology, Institute of Science & Technology, Jawaharlal Nehru Technological University Hyderabad , Hyderabad , Telangana , India.a Department of Bio-Technology , Center for Bio-Technology, Institute of Science & Technology, Jawaharlal Nehru Technological University Hyderabad , Hyderabad , Telangana , India.b Department of Microbiology , Osmania Medical College , Hyderabad , Telangana , India.a Department of Bio-Technology , Center for Bio-Technology, Institute of Science & Technology, Jawaharlal Nehru Technological University Hyderabad , Hyderabad , Telangana , India.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28219296

Citation

Banothu, Venkanna, et al. "Phytochemical Screening and Evaluation of in Vitro Antioxidant and Antimicrobial Activities of the Indigenous Medicinal Plant Albizia Odoratissima." Pharmaceutical Biology, vol. 55, no. 1, 2017, pp. 1155-1161.
Banothu V, Neelagiri C, Adepally U, et al. Phytochemical screening and evaluation of in vitro antioxidant and antimicrobial activities of the indigenous medicinal plant Albizia odoratissima. Pharm Biol. 2017;55(1):1155-1161.
Banothu, V., Neelagiri, C., Adepally, U., Lingam, J., & Bommareddy, K. (2017). Phytochemical screening and evaluation of in vitro antioxidant and antimicrobial activities of the indigenous medicinal plant Albizia odoratissima. Pharmaceutical Biology, 55(1), 1155-1161. https://doi.org/10.1080/13880209.2017.1291694
Banothu V, et al. Phytochemical Screening and Evaluation of in Vitro Antioxidant and Antimicrobial Activities of the Indigenous Medicinal Plant Albizia Odoratissima. Pharm Biol. 2017;55(1):1155-1161. PubMed PMID: 28219296.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phytochemical screening and evaluation of in vitro antioxidant and antimicrobial activities of the indigenous medicinal plant Albizia odoratissima. AU - Banothu,Venkanna, AU - Neelagiri,Chandrasekharnath, AU - Adepally,Uma, AU - Lingam,Jayalakshmi, AU - Bommareddy,Kesavaharshini, PY - 2017/2/22/entrez PY - 2017/2/22/pubmed PY - 2017/3/10/medline KW - Agar well diffusion KW - radical scavenging activity KW - secondary metabolites KW - total flavonoids KW - total phenolics SP - 1155 EP - 1161 JF - Pharmaceutical biology JO - Pharm Biol VL - 55 IS - 1 N2 - CONTEXT: Albizia odoratissima (L. f.) Benth has been used in Indian folk medicine to treat numerous inflammatory pathologies, such as leprosy, ulcers, burns and asthma. OBJECTIVE: To evaluate the antioxidant and antimicrobial properties of A. odoratissima. MATERIALS AND METHODS: Dried leaves of A. odoratissima were extracted in organic solvents (hexane, chloroform, ethyl acetate, and methanol). The total phenolic content (TPC) and total flavonoid content (TFC) were determined using the Folin-Ciocalteu and aluminum chloride colorimetric methods, respectively. The antioxidant activity was examined using 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydrogen peroxide (H2O2), 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS), and ferric reducing antioxidant power (FRAP) assays. The antibacterial activity was examined using minimum inhibitory concentration (MIC) and the minimum bacterial concentration (MBC), determined by broth microdilution method against Gram-negative bacteria (Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa, and Proteus vulgaris) and Gram-positive bacterium (Staphylococcus aureus). RESULTS: The TPC ranged from 4.40 ± 1.06 to 1166.66 ± 31.85 mg GAE/g of dry weight (DW), and the TFC ranged from 48.35 ± 3.62 to 109.74 ± 1.84 mg QE/g of DW. The IC50 values of the ethyl acetate extract for DPPH, ABTS, and H2O2 were 10.96 ± 0.40, 4.35 ± 0.07, and 163.82 ± 1.52 μg/mL, respectively. Both methanol and ethyl acetate extracts demonstrated effective antibacterial activity with MICs and MBCs values ranging 136-546 μg/mL and 273-1093 μg/mL, respectively, against the tested pathogenic species. CONCLUSIONS: The leaves of A. odoratissima showed potent free radical scavenging property and antimicrobial activity. SN - 1744-5116 UR - https://www.unboundmedicine.com/medline/citation/28219296/Phytochemical_screening_and_evaluation_of_in_vitro_antioxidant_and_antimicrobial_activities_of_the_indigenous_medicinal_plant_Albizia_odoratissima_ L2 - http://www.tandfonline.com/doi/full/10.1080/13880209.2017.1291694 DB - PRIME DP - Unbound Medicine ER -