TY - JOUR T1 - Diversification of mitochondrial genome of Daphnia galeata (Cladocera, Crustacea): Comparison with phylogenetic consideration of the complete sequences of clones isolated from five lakes in Japan. AU - Tokishita,Shin-Ichi, AU - Shibuya,Hiroyuki, AU - Kobayashi,Taku, AU - Sakamoto,Masaki, AU - Ha,Jin-Yong, AU - Yokobori,Shin-Ichi, AU - Yamagata,Hideo, AU - Hanazato,Takayuki, Y1 - 2017/02/17/ PY - 2016/10/05/received PY - 2017/01/20/revised PY - 2017/02/13/accepted PY - 2017/2/22/pubmed PY - 2017/4/12/medline PY - 2017/2/22/entrez KW - Control region KW - Daphnia galeata KW - Mitochondrial DNA KW - Phylogenetic tree KW - Positive selection KW - ka/ks ratio SP - 38 EP - 46 JF - Gene JO - Gene VL - 611 N2 - To characterize genetic diversity and gene flow among Daphnia galeata populations, the complete nucleotide (nt) sequences of the mitochondrial (mt) DNAs of D. galeata clones isolated from five lakes in Japan (Lakes Shirakaba, Suwa, Kizaki, Kasumigaura, and Biwa) were determined. Comparison of non-synonymous (amino acid altering) substitution rates with synonymous substitution rates of D. galeata mt protein-coding genes demonstrated that ATPase8 and COI genes were the most and least susceptible, respectively, to the evolutional forces selecting the aa substitutions. Several non-synonymous substitutions were found in ATPase8 and ATPase6 even in the comparison that no synonymous substitution was found. Comparison of the total number of nt variations among the mt DNAs suggested the phylogenetic relationship ((((Shirakaba/Suwa, Kizaki), Kasumigaura), Biwa), D. pulex). Maximum-likelihood analysis using the total nt sequences of mt protein-coding genes confirmed this relationship with bootstrap values higher than 98%. All the mtDNAs of the analyzed Japanese D. galeata clones contained a control region of essentially the same structure that is distinct from those of the previously reported European Daphnia species of the D. longispina complex. The two control regions of different structures spread among mtDNAs of the Japanese and European Daphnia species, respectively, probably after the divergence of the Japanese D. galeata under different selection pressures associated with their habitats. SN - 1879-0038 UR - https://www.unboundmedicine.com/medline/citation/28219780/Diversification_of_mitochondrial_genome_of_Daphnia_galeata__Cladocera_Crustacea_:_Comparison_with_phylogenetic_consideration_of_the_complete_sequences_of_clones_isolated_from_five_lakes_in_Japan_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-1119(17)30108-7 DB - PRIME DP - Unbound Medicine ER -