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The DnaJ protein OsDjA6 negatively regulates rice innate immunity to the blast fungus Magnaporthe oryzae.
Mol Plant Pathol. 2018 03; 19(3):607-614.MP

Abstract

Rice blast, caused by Magnaporthe oryzae (synonym: Pyricularia oryzae), severely reduces rice production and grain quality. The molecular mechanism of rice resistance to M. oryzae is not fully understood. In this study, we identified a chaperone DnaJ protein, OsDjA6, which is involved in basal resistance to M. oryzae in rice. The OsDjA6 protein is distributed in the entire rice cell. The expression of OsDjA6 is significantly induced in rice after infection with a compatible isolate. Silencing of OsDjA6 in transgenic rice enhances resistance to M. oryzae and also results in an increased burst of reactive oxygen species after flg22 and chitin treatments. In addition, the expression levels of WRKY45, NPR1 and PR5 are increased in OsDjA6 RNAi plants, indicating that OsDjA6 may mediate resistance by affecting the salicylic acid pathway. Finally, we found that OsDjA6 interacts directly with the E3 ligase OsZFP1 in vitro and in vivo. These results suggest that the DnaJ protein OsDjA6 negatively regulates rice innate immunity, probably via the ubiquitination proteasome degradation pathway.

Authors+Show Affiliations

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China. Department of Plant Pathology, Ohio State University, Columbus, OH, 43210, USA.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

28220688

Citation

Zhong, Xionghui, et al. "The DnaJ Protein OsDjA6 Negatively Regulates Rice Innate Immunity to the Blast Fungus Magnaporthe Oryzae." Molecular Plant Pathology, vol. 19, no. 3, 2018, pp. 607-614.
Zhong X, Yang J, Shi Y, et al. The DnaJ protein OsDjA6 negatively regulates rice innate immunity to the blast fungus Magnaporthe oryzae. Mol Plant Pathol. 2018;19(3):607-614.
Zhong, X., Yang, J., Shi, Y., Wang, X., & Wang, G. L. (2018). The DnaJ protein OsDjA6 negatively regulates rice innate immunity to the blast fungus Magnaporthe oryzae. Molecular Plant Pathology, 19(3), 607-614. https://doi.org/10.1111/mpp.12546
Zhong X, et al. The DnaJ Protein OsDjA6 Negatively Regulates Rice Innate Immunity to the Blast Fungus Magnaporthe Oryzae. Mol Plant Pathol. 2018;19(3):607-614. PubMed PMID: 28220688.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The DnaJ protein OsDjA6 negatively regulates rice innate immunity to the blast fungus Magnaporthe oryzae. AU - Zhong,Xionghui, AU - Yang,Jiuxia, AU - Shi,Yanlong, AU - Wang,Xuli, AU - Wang,Guo-Liang, Y1 - 2017/04/02/ PY - 2016/12/03/received PY - 2017/02/13/revised PY - 2017/02/17/accepted PY - 2017/2/22/pubmed PY - 2018/11/20/medline PY - 2017/2/22/entrez KW - 26S proteasome pathway KW - DnaJ protein KW - E3 ligases KW - Magnaporthe oryzae KW - Oryza sativa KW - Pyricularia oryzae KW - rice blast SP - 607 EP - 614 JF - Molecular plant pathology JO - Mol Plant Pathol VL - 19 IS - 3 N2 - Rice blast, caused by Magnaporthe oryzae (synonym: Pyricularia oryzae), severely reduces rice production and grain quality. The molecular mechanism of rice resistance to M. oryzae is not fully understood. In this study, we identified a chaperone DnaJ protein, OsDjA6, which is involved in basal resistance to M. oryzae in rice. The OsDjA6 protein is distributed in the entire rice cell. The expression of OsDjA6 is significantly induced in rice after infection with a compatible isolate. Silencing of OsDjA6 in transgenic rice enhances resistance to M. oryzae and also results in an increased burst of reactive oxygen species after flg22 and chitin treatments. In addition, the expression levels of WRKY45, NPR1 and PR5 are increased in OsDjA6 RNAi plants, indicating that OsDjA6 may mediate resistance by affecting the salicylic acid pathway. Finally, we found that OsDjA6 interacts directly with the E3 ligase OsZFP1 in vitro and in vivo. These results suggest that the DnaJ protein OsDjA6 negatively regulates rice innate immunity, probably via the ubiquitination proteasome degradation pathway. SN - 1364-3703 UR - https://www.unboundmedicine.com/medline/citation/28220688/The_DnaJ_protein_OsDjA6_negatively_regulates_rice_innate_immunity_to_the_blast_fungus_Magnaporthe_oryzae_ L2 - https://doi.org/10.1111/mpp.12546 DB - PRIME DP - Unbound Medicine ER -