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Inactivation of Escherichia coli O157:H7 and Aerobic Microorganisms in Romaine Lettuce Packaged in a Commercial Polyethylene Terephthalate Container Using Atmospheric Cold Plasma.
J Food Prot. 2017 01; 80(1):35-43.JF

Abstract

The effects of dielectric barrier discharge atmospheric cold plasma (DACP) treatment on the inactivation of Escherichia coli O157:H7 and aerobic microorganisms in romaine lettuce packaged in a conventional commercial plastic container were evaluated during storage at 4°C for 7 days. Effects investigated included the color, carbon dioxide (CO2) generation, weight loss, and surface morphology of the lettuce during storage. Romaine lettuce pieces, with or without inoculation with a cocktail of three strains of E. coli O157:H7 (~6 log CFU/g of lettuce), were packaged in a polyethylene terephthalate commercial clamshell container and treated at 34.8 kV at 1.1 kHz for 5 min by using a DACP treatment system equipped with a pin-type high-voltage electrode. Romaine lettuce samples were analyzed for inactivation of E. coli O157:H7, total mesophilic aerobes, and yeasts and molds, color, CO2 generation, weight loss, and surface morphology during storage at 4°C for 7 days. The DACP treatment reduced the initial counts of E. coli O157:H7 and total aerobic microorganisms by ~1 log CFU/g, with negligible temperature change from 24.5 ± 1.4°C to 26.6 ± 1.7°C. The reductions in the numbers of E. coli O157:H7, total mesophilic aerobes, and yeasts and molds during storage were 0.8 to 1.5, 0.7 to 1.9, and 0.9 to 1.7 log CFU/g, respectively. DACP treatment, however, did not significantly affect the color, CO2 generation, weight, and surface morphology of lettuce during storage (P > 0.05). Some mesophilic aerobic bacteria were sublethally injured by DACP treatment. The results from this study demonstrate the potential of applying DACP as a postpackaging treatment to decontaminate lettuce contained in conventional plastic packages without altering color and leaf respiration during posttreatment cold storage.

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Publisher Full Text (DOI)

Authors+Show Affiliations

U.S. Department of Agriculture, Eastern Regional Research Center, 600 Mermaid Lane, Wyndmoor, Pennsylvania 19038, USA. Department of Food Science and Technology, Seoul Women's University, 621 Hwarangro, Nowon-gu, Seoul 139-774, Republic of Korea.

U.S. Department of Agriculture, Eastern Regional Research Center, 600 Mermaid Lane, Wyndmoor, Pennsylvania 19038, USA. Department of Food Science and Technology, Seoul Women's University, 621 Hwarangro, Nowon-gu, Seoul 139-774, Republic of Korea.

U.S. Department of Agriculture, Eastern Regional Research Center, 600 Mermaid Lane, Wyndmoor, Pennsylvania 19038, USA.

U.S. Department of Agriculture, Eastern Regional Research Center, 600 Mermaid Lane, Wyndmoor, Pennsylvania 19038, USA.

U.S. Department of Agriculture, Eastern Regional Research Center, 600 Mermaid Lane, Wyndmoor, Pennsylvania 19038, USA.

U.S. Department of Agriculture, Eastern Regional Research Center, 600 Mermaid Lane, Wyndmoor, Pennsylvania 19038, USA.

U.S. Department of Agriculture, Eastern Regional Research Center, 600 Mermaid Lane, Wyndmoor, Pennsylvania 19038, USA.

MeSH

Colony Count, MicrobialEscherichia coli O157Food MicrobiologyLettucePlasma GasesPolyethylene Terephthalates

Pub Type(s)

Journal Article

Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

28221875

Citation

Min, Sea C., et al. "Inactivation of Escherichia Coli O157:H7 and Aerobic Microorganisms in Romaine Lettuce Packaged in a Commercial Polyethylene Terephthalate Container Using Atmospheric Cold Plasma." Journal of Food Protection, vol. 80, no. 1, 2017, pp. 35-43.

Min SC, Roh SH, Boyd G, et al. Inactivation of Escherichia coli O157:H7 and Aerobic Microorganisms in Romaine Lettuce Packaged in a Commercial Polyethylene Terephthalate Container Using Atmospheric Cold Plasma. J Food Prot. 2017;80(1):35-43.

Min, S. C., Roh, S. H., Boyd, G., Sites, J. E., Uknalis, J., Fan, X., & Niemira, B. A. (2017). Inactivation of Escherichia coli O157:H7 and Aerobic Microorganisms in Romaine Lettuce Packaged in a Commercial Polyethylene Terephthalate Container Using Atmospheric Cold Plasma. Journal of Food Protection, 80(1), 35-43. https://doi.org/10.4315/0362-028X.JFP-16-148

Min SC, et al. Inactivation of Escherichia Coli O157:H7 and Aerobic Microorganisms in Romaine Lettuce Packaged in a Commercial Polyethylene Terephthalate Container Using Atmospheric Cold Plasma. J Food Prot. 2017;80(1):35-43. PubMed PMID: 28221875.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Inactivation of Escherichia coli O157:H7 and Aerobic Microorganisms in Romaine Lettuce Packaged in a Commercial Polyethylene Terephthalate Container Using Atmospheric Cold Plasma. AU - Min,Sea C, AU - Roh,Si Hyeon, AU - Boyd,Glenn, AU - Sites,Joseph E, AU - Uknalis,Joseph, AU - Fan,Xuetong, AU - Niemira,Brendan A, PY - 2017/2/22/entrez PY - 2017/2/22/pubmed PY - 2017/6/6/medline KW - Cold plasma KW - Dielectric barrier discharge KW - Escherichia coli O157:H7 KW - Lettuce KW - Nonthermal processing SP - 35 EP - 43 JF - Journal of food protection JO - J Food Prot VL - 80 IS - 1 N2 - The effects of dielectric barrier discharge atmospheric cold plasma (DACP) treatment on the inactivation of Escherichia coli O157:H7 and aerobic microorganisms in romaine lettuce packaged in a conventional commercial plastic container were evaluated during storage at 4°C for 7 days. Effects investigated included the color, carbon dioxide (CO2) generation, weight loss, and surface morphology of the lettuce during storage. Romaine lettuce pieces, with or without inoculation with a cocktail of three strains of E. coli O157:H7 (~6 log CFU/g of lettuce), were packaged in a polyethylene terephthalate commercial clamshell container and treated at 34.8 kV at 1.1 kHz for 5 min by using a DACP treatment system equipped with a pin-type high-voltage electrode. Romaine lettuce samples were analyzed for inactivation of E. coli O157:H7, total mesophilic aerobes, and yeasts and molds, color, CO2 generation, weight loss, and surface morphology during storage at 4°C for 7 days. The DACP treatment reduced the initial counts of E. coli O157:H7 and total aerobic microorganisms by ~1 log CFU/g, with negligible temperature change from 24.5 ± 1.4°C to 26.6 ± 1.7°C. The reductions in the numbers of E. coli O157:H7, total mesophilic aerobes, and yeasts and molds during storage were 0.8 to 1.5, 0.7 to 1.9, and 0.9 to 1.7 log CFU/g, respectively. DACP treatment, however, did not significantly affect the color, CO2 generation, weight, and surface morphology of lettuce during storage (P > 0.05). Some mesophilic aerobic bacteria were sublethally injured by DACP treatment. The results from this study demonstrate the potential of applying DACP as a postpackaging treatment to decontaminate lettuce contained in conventional plastic packages without altering color and leaf respiration during posttreatment cold storage. SN - 1944-9097 UR - https://www.unboundmedicine.com/medline/citation/28221875/Inactivation_of_Escherichia_coli_O157:H7_and_Aerobic_Microorganisms_in_Romaine_Lettuce_Packaged_in_a_Commercial_Polyethylene_Terephthalate_Container_Using_Atmospheric_Cold_Plasma_ DB - PRIME DP - Unbound Medicine ER -