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Heterologous expression of a rice metallothionein isoform (OsMTI-1b) in Saccharomyces cerevisiae enhances cadmium, hydrogen peroxide and ethanol tolerance.
Braz J Microbiol. 2017 Jul - Sep; 48(3):537-543.BJ

Abstract

Metallothioneins are a superfamily of low-molecular-weight, cysteine (Cys)-rich proteins that are believed to play important roles in protection against metal toxicity and oxidative stress. The main purpose of this study was to investigate the effect of heterologous expression of a rice metallothionein isoform (OsMTI-1b) on the tolerance of Saccharomyces cerevisiae to Cd2+, H2O2 and ethanol stress. The gene encoding OsMTI-1b was cloned into p426GPD as a yeast expression vector. The new construct was transformed to competent cells of S. cerevisiae. After verification of heterologous expression of OsMTI-1b, the new strain and control were grown under stress conditions. In comparison to control strain, the transformed S. cerevisiae cells expressing OsMTI-1b showed more tolerance to Cd2+ and accumulated more Cd2+ ions when they were grown in the medium containing CdCl2. In addition, the heterologous expression of GST-OsMTI-1b conferred H2O2 and ethanol tolerance to S. cerevisiae cells. The results indicate that heterologous expression of plant MT isoforms can enhance the tolerance of S. cerevisiae to multiple stresses.

Authors+Show Affiliations

Isfahan University of Technology, College of Agriculture, Department of Biotechnology, Isfahan, Iran.Isfahan University of Technology, College of Agriculture, Department of Biotechnology, Isfahan, Iran. Electronic address: a.shahpiri@cc.iut.ac.ir.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28223030

Citation

Ansarypour, Zahra, and Azar Shahpiri. "Heterologous Expression of a Rice Metallothionein Isoform (OsMTI-1b) in Saccharomyces Cerevisiae Enhances Cadmium, Hydrogen Peroxide and Ethanol Tolerance." Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology], vol. 48, no. 3, 2017, pp. 537-543.
Ansarypour Z, Shahpiri A. Heterologous expression of a rice metallothionein isoform (OsMTI-1b) in Saccharomyces cerevisiae enhances cadmium, hydrogen peroxide and ethanol tolerance. Braz J Microbiol. 2017;48(3):537-543.
Ansarypour, Z., & Shahpiri, A. (2017). Heterologous expression of a rice metallothionein isoform (OsMTI-1b) in Saccharomyces cerevisiae enhances cadmium, hydrogen peroxide and ethanol tolerance. Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology], 48(3), 537-543. https://doi.org/10.1016/j.bjm.2016.10.024
Ansarypour Z, Shahpiri A. Heterologous Expression of a Rice Metallothionein Isoform (OsMTI-1b) in Saccharomyces Cerevisiae Enhances Cadmium, Hydrogen Peroxide and Ethanol Tolerance. Braz J Microbiol. 2017 Jul - Sep;48(3):537-543. PubMed PMID: 28223030.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Heterologous expression of a rice metallothionein isoform (OsMTI-1b) in Saccharomyces cerevisiae enhances cadmium, hydrogen peroxide and ethanol tolerance. AU - Ansarypour,Zahra, AU - Shahpiri,Azar, Y1 - 2017/02/04/ PY - 2016/05/01/received PY - 2016/10/12/revised PY - 2016/10/25/accepted PY - 2017/2/23/pubmed PY - 2018/3/13/medline PY - 2017/2/23/entrez KW - Cadmium KW - Ethanol KW - Hydrogen peroxide KW - Rice metallothionein KW - S. cerevisiae SP - 537 EP - 543 JF - Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] JO - Braz J Microbiol VL - 48 IS - 3 N2 - Metallothioneins are a superfamily of low-molecular-weight, cysteine (Cys)-rich proteins that are believed to play important roles in protection against metal toxicity and oxidative stress. The main purpose of this study was to investigate the effect of heterologous expression of a rice metallothionein isoform (OsMTI-1b) on the tolerance of Saccharomyces cerevisiae to Cd2+, H2O2 and ethanol stress. The gene encoding OsMTI-1b was cloned into p426GPD as a yeast expression vector. The new construct was transformed to competent cells of S. cerevisiae. After verification of heterologous expression of OsMTI-1b, the new strain and control were grown under stress conditions. In comparison to control strain, the transformed S. cerevisiae cells expressing OsMTI-1b showed more tolerance to Cd2+ and accumulated more Cd2+ ions when they were grown in the medium containing CdCl2. In addition, the heterologous expression of GST-OsMTI-1b conferred H2O2 and ethanol tolerance to S. cerevisiae cells. The results indicate that heterologous expression of plant MT isoforms can enhance the tolerance of S. cerevisiae to multiple stresses. SN - 1678-4405 UR - https://www.unboundmedicine.com/medline/citation/28223030/Heterologous_expression_of_a_rice_metallothionein_isoform__OsMTI_1b__in_Saccharomyces_cerevisiae_enhances_cadmium_hydrogen_peroxide_and_ethanol_tolerance_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/28223030/ DB - PRIME DP - Unbound Medicine ER -