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Langmuir-Schaefer film deposition onto honeycomb porous films for retinal tissue engineering.
Acta Biomater. 2017 05; 54:138-149.AB

Abstract

Age-related macular degeneration (AMD) is the leading cause of vision loss in senior citizens in the developed world. The disease is characterised by the degeneration of a specific cell layer at the back of the eye - the retinal pigment epithelium (RPE), which is essential in retinal function. The most promising therapeutic option to restore the lost vision is considered to be RPE cell transplantation. This work focuses on the development of biodegradable biomaterials with similar properties to the native Bruch's membrane as carriers for RPE cells. In particular, the breath figure (BF) method was used to create semi-permeable microporous films, which were thereafter used as the substrate for the consecutive Langmuir-Schaefer (LS) deposition of highly organised layers of collagen type I and collagen type IV. The newly developed biomaterials were further characterised in terms of surface porosity, roughness, hydrophilicity, collagen distribution, diffusion properties and hydrolytic stability. Human embryonic stem cell-derived RPE cells (hESC-RPE) cultured on the biomaterials showed good adhesion, spreading and morphology, as well as the expression of specific protein markers. Cell function was additionally confirmed by the assessment of the phagocytic capacity of hESC-RPE. Throughout the study, microporous films consistently showed better results as cell culture materials for hESC-RPE than dip-coated controls. This work demonstrates the potential of the BF-LS combined technologies to create biomimetic prosthetic Bruch's membranes for hESC-RPE transplantation.

STATEMENT OF SIGNIFICANCE

Age-related macular degeneration (AMD) is a leading cause of central blindness in developed countries, associated with the degeneration of the retinal pigment epithelium (RPE), a specific cell layer at the back of the eye. Transplantation of RPE cells derived from stem cells is considered the best option to treat these patients. In this work, we developed a cell carrier for human embryonic stem cell-derived RPE that resembled the upper layers of the membrane that naturally supports the RPE cells in the retina. The new combination of technologies employed in this study resulted in very promising materials as confirmed by our studies on cell proliferation, morphology and function.

Authors+Show Affiliations

Faculty of Biomedical Sciences and Engineering, and BioMediTech Institute, Tampere University of Technology, Tampere, Finland. Electronic address: teresa.calejo@tut.fi.Faculty of Medicine and Life Sciences, and BioMediTech Institute, University of Tampere, Tampere, Finland.Laboratory of Chemistry and Bioengineering, Tampere University of Technology, Tampere, Finland.Faculty of Biomedical Sciences and Engineering, and BioMediTech Institute, Tampere University of Technology, Tampere, Finland.Laboratory of Chemistry and Bioengineering, Tampere University of Technology, Tampere, Finland.Faculty of Medicine and Life Sciences, and BioMediTech Institute, University of Tampere, Tampere, Finland.Faculty of Biomedical Sciences and Engineering, and BioMediTech Institute, Tampere University of Technology, Tampere, Finland.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

28223209

Citation

Calejo, Maria Teresa, et al. "Langmuir-Schaefer Film Deposition Onto Honeycomb Porous Films for Retinal Tissue Engineering." Acta Biomaterialia, vol. 54, 2017, pp. 138-149.
Calejo MT, Ilmarinen T, Vuorimaa-Laukkanen E, et al. Langmuir-Schaefer film deposition onto honeycomb porous films for retinal tissue engineering. Acta Biomater. 2017;54:138-149.
Calejo, M. T., Ilmarinen, T., Vuorimaa-Laukkanen, E., Talvitie, E., Hakola, H. M., Skottman, H., & Kellomäki, M. (2017). Langmuir-Schaefer film deposition onto honeycomb porous films for retinal tissue engineering. Acta Biomaterialia, 54, 138-149. https://doi.org/10.1016/j.actbio.2017.02.035
Calejo MT, et al. Langmuir-Schaefer Film Deposition Onto Honeycomb Porous Films for Retinal Tissue Engineering. Acta Biomater. 2017;54:138-149. PubMed PMID: 28223209.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Langmuir-Schaefer film deposition onto honeycomb porous films for retinal tissue engineering. AU - Calejo,Maria Teresa, AU - Ilmarinen,Tanja, AU - Vuorimaa-Laukkanen,Elina, AU - Talvitie,Elina, AU - Hakola,Hanna M, AU - Skottman,Heli, AU - Kellomäki,Minna, Y1 - 2017/02/20/ PY - 2016/09/13/received PY - 2017/01/31/revised PY - 2017/02/16/accepted PY - 2017/2/23/pubmed PY - 2018/2/15/medline PY - 2017/2/23/entrez KW - Honeycomb films KW - Langmuir-Schaefer films KW - Pluripotent stem cells KW - Retinal pigment epithelium KW - Tissue engineering SP - 138 EP - 149 JF - Acta biomaterialia JO - Acta Biomater VL - 54 N2 - : Age-related macular degeneration (AMD) is the leading cause of vision loss in senior citizens in the developed world. The disease is characterised by the degeneration of a specific cell layer at the back of the eye - the retinal pigment epithelium (RPE), which is essential in retinal function. The most promising therapeutic option to restore the lost vision is considered to be RPE cell transplantation. This work focuses on the development of biodegradable biomaterials with similar properties to the native Bruch's membrane as carriers for RPE cells. In particular, the breath figure (BF) method was used to create semi-permeable microporous films, which were thereafter used as the substrate for the consecutive Langmuir-Schaefer (LS) deposition of highly organised layers of collagen type I and collagen type IV. The newly developed biomaterials were further characterised in terms of surface porosity, roughness, hydrophilicity, collagen distribution, diffusion properties and hydrolytic stability. Human embryonic stem cell-derived RPE cells (hESC-RPE) cultured on the biomaterials showed good adhesion, spreading and morphology, as well as the expression of specific protein markers. Cell function was additionally confirmed by the assessment of the phagocytic capacity of hESC-RPE. Throughout the study, microporous films consistently showed better results as cell culture materials for hESC-RPE than dip-coated controls. This work demonstrates the potential of the BF-LS combined technologies to create biomimetic prosthetic Bruch's membranes for hESC-RPE transplantation. STATEMENT OF SIGNIFICANCE: Age-related macular degeneration (AMD) is a leading cause of central blindness in developed countries, associated with the degeneration of the retinal pigment epithelium (RPE), a specific cell layer at the back of the eye. Transplantation of RPE cells derived from stem cells is considered the best option to treat these patients. In this work, we developed a cell carrier for human embryonic stem cell-derived RPE that resembled the upper layers of the membrane that naturally supports the RPE cells in the retina. The new combination of technologies employed in this study resulted in very promising materials as confirmed by our studies on cell proliferation, morphology and function. SN - 1878-7568 UR - https://www.unboundmedicine.com/medline/citation/28223209/Langmuir_Schaefer_film_deposition_onto_honeycomb_porous_films_for_retinal_tissue_engineering_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1742-7061(17)30140-X DB - PRIME DP - Unbound Medicine ER -