Tags

Type your tag names separated by a space and hit enter

Regulation of the vir genes of Agrobacterium tumefaciens plasmid pTiC58.
J Bacteriol. 1987 Nov; 169(11):5101-12.JB

Abstract

The virulence (vir) region of pTiC58 was screened for promoter activities by using gene fusions to a promoterless lux operon in the broad-host-range vector pUCD615. Active vir fragments contained the strongly acetosyringone-inducible promoters of virB, virC, virD, and virE and the weakly inducible promoters of virA and virG. Identical induction patterns were obtained with freshly sliced carrot disks, suggesting that an inducer is released after plant tissue is wounded. Optimal conditions for vir gene induction were pH 5.7 for 50 microM acetosyringone or sinapic acid. The induction of virB and virE by acetosyringone was strictly dependent on intact virA and virG loci. An increase in the copy number of virG resulted in a proportional, acetosyringone-independent increase in vir gene expression, and a further increase occurred only if an inducing compound and virA were present.

Authors+Show Affiliations

Department of Plant Pathology, University of California, Davis 95616.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

2822665

Citation

Rogowsky, P M., et al. "Regulation of the Vir Genes of Agrobacterium Tumefaciens Plasmid PTiC58." Journal of Bacteriology, vol. 169, no. 11, 1987, pp. 5101-12.
Rogowsky PM, Close TJ, Chimera JA, et al. Regulation of the vir genes of Agrobacterium tumefaciens plasmid pTiC58. J Bacteriol. 1987;169(11):5101-12.
Rogowsky, P. M., Close, T. J., Chimera, J. A., Shaw, J. J., & Kado, C. I. (1987). Regulation of the vir genes of Agrobacterium tumefaciens plasmid pTiC58. Journal of Bacteriology, 169(11), 5101-12.
Rogowsky PM, et al. Regulation of the Vir Genes of Agrobacterium Tumefaciens Plasmid PTiC58. J Bacteriol. 1987;169(11):5101-12. PubMed PMID: 2822665.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Regulation of the vir genes of Agrobacterium tumefaciens plasmid pTiC58. AU - Rogowsky,P M, AU - Close,T J, AU - Chimera,J A, AU - Shaw,J J, AU - Kado,C I, PY - 1987/11/1/pubmed PY - 1987/11/1/medline PY - 1987/11/1/entrez SP - 5101 EP - 12 JF - Journal of bacteriology JO - J. Bacteriol. VL - 169 IS - 11 N2 - The virulence (vir) region of pTiC58 was screened for promoter activities by using gene fusions to a promoterless lux operon in the broad-host-range vector pUCD615. Active vir fragments contained the strongly acetosyringone-inducible promoters of virB, virC, virD, and virE and the weakly inducible promoters of virA and virG. Identical induction patterns were obtained with freshly sliced carrot disks, suggesting that an inducer is released after plant tissue is wounded. Optimal conditions for vir gene induction were pH 5.7 for 50 microM acetosyringone or sinapic acid. The induction of virB and virE by acetosyringone was strictly dependent on intact virA and virG loci. An increase in the copy number of virG resulted in a proportional, acetosyringone-independent increase in vir gene expression, and a further increase occurred only if an inducing compound and virA were present. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/2822665/Regulation_of_the_vir_genes_of_Agrobacterium_tumefaciens_plasmid_pTiC58_ L2 - http://jb.asm.org/cgi/pmidlookup?view=long&pmid=2822665 DB - PRIME DP - Unbound Medicine ER -