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Protective effect of a polysaccharide from Anoectochilus roxburghii against carbon tetrachloride-induced acute liver injury in mice.
J Ethnopharmacol. 2017 Mar 22; 200:124-135.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Anoectochilus roxburghii (Wall.) Lindl. is traditionally used for the treatment of various types of chronic and acute hepatitis in China. Considering that Anoectochilus roxburghii polysaccharide (ARPT) is the main constituent of Anoectochilus roxburghii, the present study was designed to investigate the hepatoprotective effect of ARPT and its possible mechanism in carbon tetrachloride (CCl4)-induced mice.

MATERIAL AND METHODS

The hepatoprotective activity of ARPT (150, 300 and 500mg/kg) were investigated on CCl4-induced acute liver damage in mice. The activities of alanine transaminase (ALT), aspartate transaminase (AST) were determined in serum. The hepatic levels of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) were measured in liver homogenates. The levels of cytochrome P450 sub family 2E1 (CYP2E1), tumor necrosis factor alpha (TNF-α), interleukin-1 beta (IL-1β), interleukin-6 (IL-6), monocyte chemoattractant protein-1 (MCP-1), macrophage inflammatory protein-2 (MIP-2), KC (Murine IL-8 ortholog), transforming growth factor-beta1 (TGF-β1), Bcl-2 and Bax were measured by quantitative real-time polymerase chain reaction (qRT-PCR). The expressions of CYP2E1, nuclear factor-kappa B (NF-κB) p65 and caspase-3 were evaluated by western blot assays. The hepatic levels of TNF-α, IL-6, MIP-2 and TGF-β1 were measured by enzyme-linked immunosorbent assay (ELISA). Furthermore, histopathological observation and terminal-deoxynucleoitidyl transferase mediated nick end labeling assay (TUNEL) were carried out on the separated livers of mice.

RESULTS

ARPT significantly decreased serum ALT and AST activities, hepatic MDA level, and markedly enhanced antioxidant enzyme (SOD, CAT and GSH-Px) activities and GSH level in hepatic tissue, in a dose-dependent manner, when compared to the model group. Histopathological observation revealed the hepatoprotective effect of ARPT against the damage. Furthermore, ARPT remarkably inhibited CYP2E1 mRNA expression, decreased NF-κB p65 expression and therefore to prevent the secretion of pro-inflammatory cytokines (TNF-α and IL-6) and chemokines (MCP-1, MIP-2 and KC), suppressed TGF-β1 expression and hepatocytes apoptosis. Moreover, ARPT could prevent DNA fragmentation based on TUNEL assay results.

CONCLUSION

These findings suggested that ARPT possessed hepatoprotective effect against CCl4-induced hepatotoxicity in mice and the action might in part be through reducing oxidative stress, inflammation and apoptosis.

Authors+Show Affiliations

College of Horticulture, Fujian Agricultural and Forestry University, 350002 Fuzhou, China; Fujian Key Laboratory of Physiology and Biochemistry for Subtropical Plant, Fujian Institute of Subtropical Botany, 361006 Xiamen, China.College of Horticulture, Fujian Agricultural and Forestry University, 350002 Fuzhou, China; Fujian Key Laboratory of Physiology and Biochemistry for Subtropical Plant, Fujian Institute of Subtropical Botany, 361006 Xiamen, China.College of Horticulture, Fujian Agricultural and Forestry University, 350002 Fuzhou, China.College of Horticulture, Fujian Agricultural and Forestry University, 350002 Fuzhou, China; Fujian Key Laboratory of Physiology and Biochemistry for Subtropical Plant, Fujian Institute of Subtropical Botany, 361006 Xiamen, China.College of Horticulture, Fujian Agricultural and Forestry University, 350002 Fuzhou, China; Fujian Key Laboratory of Physiology and Biochemistry for Subtropical Plant, Fujian Institute of Subtropical Botany, 361006 Xiamen, China.Fujian Key Laboratory of Physiology and Biochemistry for Subtropical Plant, Fujian Institute of Subtropical Botany, 361006 Xiamen, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28229921

Citation

Zeng, Biyu, et al. "Protective Effect of a Polysaccharide From Anoectochilus Roxburghii Against Carbon Tetrachloride-induced Acute Liver Injury in Mice." Journal of Ethnopharmacology, vol. 200, 2017, pp. 124-135.
Zeng B, Su M, Chen Q, et al. Protective effect of a polysaccharide from Anoectochilus roxburghii against carbon tetrachloride-induced acute liver injury in mice. J Ethnopharmacol. 2017;200:124-135.
Zeng, B., Su, M., Chen, Q., Chang, Q., Wang, W., & Li, H. (2017). Protective effect of a polysaccharide from Anoectochilus roxburghii against carbon tetrachloride-induced acute liver injury in mice. Journal of Ethnopharmacology, 200, 124-135. https://doi.org/10.1016/j.jep.2017.02.018
Zeng B, et al. Protective Effect of a Polysaccharide From Anoectochilus Roxburghii Against Carbon Tetrachloride-induced Acute Liver Injury in Mice. J Ethnopharmacol. 2017 Mar 22;200:124-135. PubMed PMID: 28229921.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effect of a polysaccharide from Anoectochilus roxburghii against carbon tetrachloride-induced acute liver injury in mice. AU - Zeng,Biyu, AU - Su,Minghua, AU - Chen,Qingxi, AU - Chang,Qiang, AU - Wang,Wei, AU - Li,Huihua, Y1 - 2017/02/14/ PY - 2016/08/30/received PY - 2017/01/24/revised PY - 2017/02/12/accepted PY - 2017/2/24/pubmed PY - 2017/7/20/medline PY - 2017/2/24/entrez KW - Acute liver injury KW - Anoectochilus roxburghii KW - Carbon tetrachloride KW - Hepatoprotective effect KW - Polysaccharide SP - 124 EP - 135 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 200 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Anoectochilus roxburghii (Wall.) Lindl. is traditionally used for the treatment of various types of chronic and acute hepatitis in China. Considering that Anoectochilus roxburghii polysaccharide (ARPT) is the main constituent of Anoectochilus roxburghii, the present study was designed to investigate the hepatoprotective effect of ARPT and its possible mechanism in carbon tetrachloride (CCl4)-induced mice. MATERIAL AND METHODS: The hepatoprotective activity of ARPT (150, 300 and 500mg/kg) were investigated on CCl4-induced acute liver damage in mice. The activities of alanine transaminase (ALT), aspartate transaminase (AST) were determined in serum. The hepatic levels of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) were measured in liver homogenates. The levels of cytochrome P450 sub family 2E1 (CYP2E1), tumor necrosis factor alpha (TNF-α), interleukin-1 beta (IL-1β), interleukin-6 (IL-6), monocyte chemoattractant protein-1 (MCP-1), macrophage inflammatory protein-2 (MIP-2), KC (Murine IL-8 ortholog), transforming growth factor-beta1 (TGF-β1), Bcl-2 and Bax were measured by quantitative real-time polymerase chain reaction (qRT-PCR). The expressions of CYP2E1, nuclear factor-kappa B (NF-κB) p65 and caspase-3 were evaluated by western blot assays. The hepatic levels of TNF-α, IL-6, MIP-2 and TGF-β1 were measured by enzyme-linked immunosorbent assay (ELISA). Furthermore, histopathological observation and terminal-deoxynucleoitidyl transferase mediated nick end labeling assay (TUNEL) were carried out on the separated livers of mice. RESULTS: ARPT significantly decreased serum ALT and AST activities, hepatic MDA level, and markedly enhanced antioxidant enzyme (SOD, CAT and GSH-Px) activities and GSH level in hepatic tissue, in a dose-dependent manner, when compared to the model group. Histopathological observation revealed the hepatoprotective effect of ARPT against the damage. Furthermore, ARPT remarkably inhibited CYP2E1 mRNA expression, decreased NF-κB p65 expression and therefore to prevent the secretion of pro-inflammatory cytokines (TNF-α and IL-6) and chemokines (MCP-1, MIP-2 and KC), suppressed TGF-β1 expression and hepatocytes apoptosis. Moreover, ARPT could prevent DNA fragmentation based on TUNEL assay results. CONCLUSION: These findings suggested that ARPT possessed hepatoprotective effect against CCl4-induced hepatotoxicity in mice and the action might in part be through reducing oxidative stress, inflammation and apoptosis. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/28229921/Protective_effect_of_a_polysaccharide_from_Anoectochilus_roxburghii_against_carbon_tetrachloride_induced_acute_liver_injury_in_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(17)30560-3 DB - PRIME DP - Unbound Medicine ER -