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Analysis of the Total Biflavonoids Extract from Selaginella doederleinii by HPLC-QTOF-MS and Its In Vitro and In Vivo Anticancer Effects.
Molecules 2017; 22(2)M

Abstract

Selaginella doederleinii Hieron has been traditionally used as a folk antitumor herbal medicine in China. In this paper, the phytochemical components of the total biflavonoids extract from S. doederleinii were studied by using high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight mass spectrometry (HPLC-ESI-QTOF MS/MS) in negative ion mode, and their in vitro and in vivo anticancer effects were evaluated. Four types of biflavonoids from S. doederleinii, including IC3'-IIC8'', IC3'-IIC6'', IC3'-IIC3''', and C-O linked biflavonoids were examined originally using QTOF MS/MS. The fragmentation behavior of IC3'-IIC3''' linked biflavonoids was reported for the first time. A total of twenty biflavonoids were identified or tentatively characterized and eight biflavonoids were found from S. doederleinii for the first time. Furthermore, the 3-(4,5-Dimethyl-2-thizolyl)-2,5-diphenyltertazolium bromide (MTT) assay and xenograft model of mouse lewis lung cancer(LLC) in male C57BL/6 mice revealed favorable anticancer properties of the total biflavonoids extracts from S. doederleinii. The results of this work could provide useful knowledge for the identification of biflavonoids in herbal samples and further insights into the chemopreventive function of this plant.

Authors+Show Affiliations

Department of Pharmaceutical Analysis, School of Pharmacy, Fujian Medical University, Fuzhou 350122, China. yauhung@126.com.Department of Pharmaceutical Analysis, School of Pharmacy, Fujian Medical University, Fuzhou 350122, China. chanbing@foxmail.com.Department of Pharmaceutical Analysis, School of Pharmacy, Fujian Medical University, Fuzhou 350122, China. zhangyyfjmu@sina.com.Department of Pharmaceutical Analysis, School of Pharmacy, Fujian Medical University, Fuzhou 350122, China. hgou2014@sina.com.Department of Pharmaceutical Analysis, School of Pharmacy, Fujian Medical University, Fuzhou 350122, China. yuxiangli2014@sina.com.Department of Pharmaceutical Analysis, School of Pharmacy, Fujian Medical University, Fuzhou 350122, China. lsg941@sina.com.Department of Traditional Chinese Medicine Resource and Bee Products, Fujian Agriculture and Forestry University, Fuzhou 350002, China. peiyshi@126.com.Department of Pharmaceutical Analysis, School of Pharmacy, Fujian Medical University, Fuzhou 350122, China. xhlin1963@sina.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28230759

Citation

Yao, Hong, et al. "Analysis of the Total Biflavonoids Extract From Selaginella Doederleinii By HPLC-QTOF-MS and Its in Vitro and in Vivo Anticancer Effects." Molecules (Basel, Switzerland), vol. 22, no. 2, 2017.
Yao H, Chen B, Zhang Y, et al. Analysis of the Total Biflavonoids Extract from Selaginella doederleinii by HPLC-QTOF-MS and Its In Vitro and In Vivo Anticancer Effects. Molecules. 2017;22(2).
Yao, H., Chen, B., Zhang, Y., Ou, H., Li, Y., Li, S., ... Lin, X. (2017). Analysis of the Total Biflavonoids Extract from Selaginella doederleinii by HPLC-QTOF-MS and Its In Vitro and In Vivo Anticancer Effects. Molecules (Basel, Switzerland), 22(2), doi:10.3390/molecules22020325.
Yao H, et al. Analysis of the Total Biflavonoids Extract From Selaginella Doederleinii By HPLC-QTOF-MS and Its in Vitro and in Vivo Anticancer Effects. Molecules. 2017 Feb 20;22(2) PubMed PMID: 28230759.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of the Total Biflavonoids Extract from Selaginella doederleinii by HPLC-QTOF-MS and Its In Vitro and In Vivo Anticancer Effects. AU - Yao,Hong, AU - Chen,Bing, AU - Zhang,Yanyan, AU - Ou,Huigen, AU - Li,Yuxiang, AU - Li,Shaoguang, AU - Shi,Peiying, AU - Lin,Xinhua, Y1 - 2017/02/20/ PY - 2017/01/16/received PY - 2017/02/09/revised PY - 2017/02/14/accepted PY - 2017/2/24/entrez PY - 2017/2/24/pubmed PY - 2017/5/10/medline KW - HPLC-ESI-QTOF MS/MS KW - LLC mouse model KW - Selaginella doederleinii KW - biflavonoids KW - mouse lewis lung cancer (LLC) cell KW - murine B16 melanoma cell JF - Molecules (Basel, Switzerland) JO - Molecules VL - 22 IS - 2 N2 - Selaginella doederleinii Hieron has been traditionally used as a folk antitumor herbal medicine in China. In this paper, the phytochemical components of the total biflavonoids extract from S. doederleinii were studied by using high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight mass spectrometry (HPLC-ESI-QTOF MS/MS) in negative ion mode, and their in vitro and in vivo anticancer effects were evaluated. Four types of biflavonoids from S. doederleinii, including IC3'-IIC8'', IC3'-IIC6'', IC3'-IIC3''', and C-O linked biflavonoids were examined originally using QTOF MS/MS. The fragmentation behavior of IC3'-IIC3''' linked biflavonoids was reported for the first time. A total of twenty biflavonoids were identified or tentatively characterized and eight biflavonoids were found from S. doederleinii for the first time. Furthermore, the 3-(4,5-Dimethyl-2-thizolyl)-2,5-diphenyltertazolium bromide (MTT) assay and xenograft model of mouse lewis lung cancer(LLC) in male C57BL/6 mice revealed favorable anticancer properties of the total biflavonoids extracts from S. doederleinii. The results of this work could provide useful knowledge for the identification of biflavonoids in herbal samples and further insights into the chemopreventive function of this plant. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/28230759/Analysis_of_the_Total_Biflavonoids_Extract_from_Selaginella_doederleinii_by_HPLC_QTOF_MS_and_Its_In_Vitro_and_In_Vivo_Anticancer_Effects_ L2 - http://www.mdpi.com/resolver?pii=molecules22020325 DB - PRIME DP - Unbound Medicine ER -