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Preparation of a silica stationary phase co-functionalized with Wulff-type phenylboronate and C12 for mixed-mode liquid chromatography.
Anal Chim Acta 2017; 962:104-113AC

Abstract

A silica stationary phase was designed and synthesized through the co-functionalization of silica with Wulff-type phenylboronate and C12 for mixed-mode liquid chromatography applications. The as-synthesized stationary phase was characterized by elemental analysis and Fourier Transform-InfraRed Spectroscopy (FT-IR). Retention mechanisms, including boronate affinity (BA), reversed-phase (RP) and anion-exchange (AE), were involved. Retention mechanism switching was easily realized by adjustment of the mobile phase constitution. Cis-diol compounds could be selectively captured under neutral conditions in BA mode and off-line separated in RP mode. Neutral, basic, acidic and amphiprotic compounds were chromatographed on the column in RP chromatography, while inorganic anions were chromatographed in AE chromatography to characterize the mixed-mode nature of the prepared stationary phase. In addition, the RP performance was compared with an octadecyl silica column in terms of column efficiency (N/m), asymmetry factor (Af), retention factor (k) and resolution (Rs). The prepared stationary phase offered multiple interactions with analytes in addition to hydrophobic interactions under RP elution conditions. Based on the mixed-mode properties, off-line 2D-LC, for selective capture and separation of urinary nucleosides, was successfully realized on a single column, demonstrating its powerful application potential for complex samples.

Authors+Show Affiliations

School of Chemistry and Chemical Engineering, Yancheng Institute of Technology, Yancheng, Jiangsu, 224000, China.School of Chemistry and Chemical Engineering, Yancheng Institute of Technology, Yancheng, Jiangsu, 224000, China.Yancheng Entry-Exit Inspection and Quarantine Bureau, Yancheng, 224000, China.Huai'an Entry-Exit Inspection and Quarantine Bureau, Huai'an, 223001, China.School of Chemistry and Chemical Engineering, Yancheng Institute of Technology, Yancheng, Jiangsu, 224000, China.School of Chemistry and Chemical Engineering, Yancheng Institute of Technology, Yancheng, Jiangsu, 224000, China.School of Chemistry and Chemical Engineering, Yancheng Institute of Technology, Yancheng, Jiangsu, 224000, China.School of Chemistry and Chemical Engineering, Yancheng Institute of Technology, Yancheng, Jiangsu, 224000, China. Electronic address: wangw@ycit.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28231874

Citation

Li, Hengye, et al. "Preparation of a Silica Stationary Phase Co-functionalized With Wulff-type Phenylboronate and C12 for Mixed-mode Liquid Chromatography." Analytica Chimica Acta, vol. 962, 2017, pp. 104-113.
Li H, Zhang X, Zhang L, et al. Preparation of a silica stationary phase co-functionalized with Wulff-type phenylboronate and C12 for mixed-mode liquid chromatography. Anal Chim Acta. 2017;962:104-113.
Li, H., Zhang, X., Zhang, L., Wang, X., Kong, F., Fan, D., ... Wang, W. (2017). Preparation of a silica stationary phase co-functionalized with Wulff-type phenylboronate and C12 for mixed-mode liquid chromatography. Analytica Chimica Acta, 962, pp. 104-113. doi:10.1016/j.aca.2017.01.028.
Li H, et al. Preparation of a Silica Stationary Phase Co-functionalized With Wulff-type Phenylboronate and C12 for Mixed-mode Liquid Chromatography. Anal Chim Acta. 2017 Apr 15;962:104-113. PubMed PMID: 28231874.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Preparation of a silica stationary phase co-functionalized with Wulff-type phenylboronate and C12 for mixed-mode liquid chromatography. AU - Li,Hengye, AU - Zhang,Xuemeng, AU - Zhang,Lin, AU - Wang,Xiaojin, AU - Kong,Fenying, AU - Fan,Dahe, AU - Li,Lei, AU - Wang,Wei, Y1 - 2017/01/30/ PY - 2016/08/11/received PY - 2017/01/14/revised PY - 2017/01/18/accepted PY - 2017/2/25/entrez PY - 2017/2/25/pubmed PY - 2018/2/13/medline KW - Boronate affinity chromatography KW - Mixed-mode chromatography KW - Reversed-phase chromatography KW - Stationary phase KW - Urinary nucleosides SP - 104 EP - 113 JF - Analytica chimica acta JO - Anal. Chim. Acta VL - 962 N2 - A silica stationary phase was designed and synthesized through the co-functionalization of silica with Wulff-type phenylboronate and C12 for mixed-mode liquid chromatography applications. The as-synthesized stationary phase was characterized by elemental analysis and Fourier Transform-InfraRed Spectroscopy (FT-IR). Retention mechanisms, including boronate affinity (BA), reversed-phase (RP) and anion-exchange (AE), were involved. Retention mechanism switching was easily realized by adjustment of the mobile phase constitution. Cis-diol compounds could be selectively captured under neutral conditions in BA mode and off-line separated in RP mode. Neutral, basic, acidic and amphiprotic compounds were chromatographed on the column in RP chromatography, while inorganic anions were chromatographed in AE chromatography to characterize the mixed-mode nature of the prepared stationary phase. In addition, the RP performance was compared with an octadecyl silica column in terms of column efficiency (N/m), asymmetry factor (Af), retention factor (k) and resolution (Rs). The prepared stationary phase offered multiple interactions with analytes in addition to hydrophobic interactions under RP elution conditions. Based on the mixed-mode properties, off-line 2D-LC, for selective capture and separation of urinary nucleosides, was successfully realized on a single column, demonstrating its powerful application potential for complex samples. SN - 1873-4324 UR - https://www.unboundmedicine.com/medline/citation/28231874/Preparation_of_a_silica_stationary_phase_co_functionalized_with_Wulff_type_phenylboronate_and_C12_for_mixed_mode_liquid_chromatography_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2670(17)30119-8 DB - PRIME DP - Unbound Medicine ER -