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Phytochemical Ginkgolide B Attenuates Amyloid-β1-42 Induced Oxidative Damage and Altered Cellular Responses in Human Neuroblastoma SH-SY5Y Cells.
J Alzheimers Dis. 2017; 60(s1):S25-S40.JA

Abstract

Oxidative stress is an upsurge in reactive oxygen/nitrogen species (ROS/RNS), which aggravates damage to cellular components viz. lipids, proteins, and nucleic acids resulting in impaired cellular functions and neurological pathologies including Alzheimer's disease (AD). In the present study, we have examined amyloid-β (Aβ)-induced oxidative stress responses, a major cause for AD, in the undifferentiated and differentiated human neuroblastoma SH-SY5Y cells. Aβ1-42-induced oxidative damage was evaluated on lipids by lipid peroxidation; proteins by protein carbonyls; antioxidant status by SOD and GSH enzyme activities; and DNA and RNA damage levels by evaluating the number of AP sites and 8-OHG base damages produced. In addition, the neuro-protective role of the phytochemical ginkgolide B (GB) in countering Aβ1-42-induced oxidative stress was assessed. We report that the differentiated cells are highly vulnerable to Aβ1-42-induced oxidative stress events as exerted by the deposition of Aβ in AD. Results of the current study suggest that the pre-treatment of GB, followed by Aβ1-42 treatment for 24 h, displayed neuro-protective potential, which countered Aβ1-42-induced oxidative stress responses in both undifferentiated and differentiated SH-SY5Y neuronal cells by: 1) hampering production of ROS and RNS; 2) reducing lipid peroxidation; 3) decreasing protein carbonyl content; 4) restoring antioxidant activities of SOD and GSH enzymes; and 5) maintaining genome integrity by reducing the oxidative DNA and RNA base damages. In conclusion, Aβ1-42 induces oxidative damage to the cellular biomolecules, which are associated with AD pathology, and are protected by the pre-treatment of GB against Aβ-toxicity. Taken together, this study advocates for phytochemical-based therapeutic interventions against AD.

Authors+Show Affiliations

Centre for Animal Sciences, School of Basic and Applied Sciences, Central University of Punjab, Bathinda, Punjab, India.Centre for Biochemistry and Microbial Sciences, School of Basic and Applied Sciences, Central University of Punjab, Bathinda, Punjab, India.Centre for Animal Sciences, School of Basic and Applied Sciences, Central University of Punjab, Bathinda, Punjab, India.Centre for Biochemistry and Microbial Sciences, School of Basic and Applied Sciences, Central University of Punjab, Bathinda, Punjab, India.Centre for Animal Sciences, School of Basic and Applied Sciences, Central University of Punjab, Bathinda, Punjab, India.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28234255

Citation

Gill, Iqbal, et al. "Phytochemical Ginkgolide B Attenuates Amyloid-β1-42 Induced Oxidative Damage and Altered Cellular Responses in Human Neuroblastoma SH-SY5Y Cells." Journal of Alzheimer's Disease : JAD, vol. 60, no. s1, 2017, pp. S25-S40.
Gill I, Kaur S, Kaur N, et al. Phytochemical Ginkgolide B Attenuates Amyloid-β1-42 Induced Oxidative Damage and Altered Cellular Responses in Human Neuroblastoma SH-SY5Y Cells. J Alzheimers Dis. 2017;60(s1):S25-S40.
Gill, I., Kaur, S., Kaur, N., Dhiman, M., & Mantha, A. K. (2017). Phytochemical Ginkgolide B Attenuates Amyloid-β1-42 Induced Oxidative Damage and Altered Cellular Responses in Human Neuroblastoma SH-SY5Y Cells. Journal of Alzheimer's Disease : JAD, 60(s1), S25-S40. https://doi.org/10.3233/JAD-161086
Gill I, et al. Phytochemical Ginkgolide B Attenuates Amyloid-β1-42 Induced Oxidative Damage and Altered Cellular Responses in Human Neuroblastoma SH-SY5Y Cells. J Alzheimers Dis. 2017;60(s1):S25-S40. PubMed PMID: 28234255.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phytochemical Ginkgolide B Attenuates Amyloid-β1-42 Induced Oxidative Damage and Altered Cellular Responses in Human Neuroblastoma SH-SY5Y Cells. AU - Gill,Iqbal, AU - Kaur,Sukhchain, AU - Kaur,Navrattan, AU - Dhiman,Monisha, AU - Mantha,Anil K, PY - 2017/2/25/pubmed PY - 2018/4/7/medline PY - 2017/2/25/entrez KW - Aβ1-42 KW - DNA/RNA base damage KW - amyloid-beta KW - antioxidants KW - ginkgolide B KW - neuronal differentiation KW - oxidative stress SP - S25 EP - S40 JF - Journal of Alzheimer's disease : JAD JO - J Alzheimers Dis VL - 60 IS - s1 N2 - Oxidative stress is an upsurge in reactive oxygen/nitrogen species (ROS/RNS), which aggravates damage to cellular components viz. lipids, proteins, and nucleic acids resulting in impaired cellular functions and neurological pathologies including Alzheimer's disease (AD). In the present study, we have examined amyloid-β (Aβ)-induced oxidative stress responses, a major cause for AD, in the undifferentiated and differentiated human neuroblastoma SH-SY5Y cells. Aβ1-42-induced oxidative damage was evaluated on lipids by lipid peroxidation; proteins by protein carbonyls; antioxidant status by SOD and GSH enzyme activities; and DNA and RNA damage levels by evaluating the number of AP sites and 8-OHG base damages produced. In addition, the neuro-protective role of the phytochemical ginkgolide B (GB) in countering Aβ1-42-induced oxidative stress was assessed. We report that the differentiated cells are highly vulnerable to Aβ1-42-induced oxidative stress events as exerted by the deposition of Aβ in AD. Results of the current study suggest that the pre-treatment of GB, followed by Aβ1-42 treatment for 24 h, displayed neuro-protective potential, which countered Aβ1-42-induced oxidative stress responses in both undifferentiated and differentiated SH-SY5Y neuronal cells by: 1) hampering production of ROS and RNS; 2) reducing lipid peroxidation; 3) decreasing protein carbonyl content; 4) restoring antioxidant activities of SOD and GSH enzymes; and 5) maintaining genome integrity by reducing the oxidative DNA and RNA base damages. In conclusion, Aβ1-42 induces oxidative damage to the cellular biomolecules, which are associated with AD pathology, and are protected by the pre-treatment of GB against Aβ-toxicity. Taken together, this study advocates for phytochemical-based therapeutic interventions against AD. SN - 1875-8908 UR - https://www.unboundmedicine.com/medline/citation/28234255/Phytochemical_Ginkgolide_B_Attenuates_Amyloid_β1_42_Induced_Oxidative_Damage_and_Altered_Cellular_Responses_in_Human_Neuroblastoma_SH_SY5Y_Cells_ DB - PRIME DP - Unbound Medicine ER -