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Characterization of pathogenesis of and immune response to Burkholderia pseudomallei K96243 using both inhalational and intraperitoneal infection models in BALB/c and C57BL/6 mice.
PLoS One. 2017; 12(2):e0172627.Plos

Abstract

Burkholderia pseudomallei, the etiologic agent of melioidosis, is a Gram negative bacterium designated as a Tier 1 threat. This bacterium is known to be endemic in Southeast Asia and Northern Australia and can infect humans and animals by several routes. Inhalational melioidosis has been associated with monsoonal rains in endemic areas and is also a significant concern in the biodefense community. There are currently no effective vaccines for B. pseudomallei and antibiotic treatment can be hampered by non-specific symptomology and also the high rate of naturally occurring antibiotic resistant strains. Well-characterized animal models will be essential when selecting novel medical countermeasures for evaluation prior to human clinical trials. Here, we further characterize differences between the responses of BALB/c and C57BL/6 mice when challenged with low doses of a low-passage and well-defined stock of B. pseudomallei K96243 via either intraperitoneal or aerosol routes of exposure. Before challenge, mice were implanted with a transponder to collect body temperature readings, and daily body weights were also recorded. Mice were euthanized on select days for pathological analyses and determination of the bacterial burden in selected tissues (blood, lungs, liver, and spleen). Additionally, spleen homogenate and sera samples were analyzed to better characterize the host immune response after infection with aerosolized bacteria. These clinical, pathological, and immunological data highlighted and confirmed important similarities and differences between these murine models and exposure routes.

Authors+Show Affiliations

Pathology Division, United States Army Medical Research Institute of Infectious Diseases (USAMRIID), Fort Detrick, Frederick, MD, United States of America.Bacteriology Division, USAMRIID, Fort Detrick, Frederick, MD, United States of America.Bacteriology Division, USAMRIID, Fort Detrick, Frederick, MD, United States of America.Bacteriology Division, USAMRIID, Fort Detrick, Frederick, MD, United States of America.Bacteriology Division, USAMRIID, Fort Detrick, Frederick, MD, United States of America.Bacteriology Division, USAMRIID, Fort Detrick, Frederick, MD, United States of America.Bacteriology Division, USAMRIID, Fort Detrick, Frederick, MD, United States of America.Bacteriology Division, USAMRIID, Fort Detrick, Frederick, MD, United States of America.Bacteriology Division, USAMRIID, Fort Detrick, Frederick, MD, United States of America.Bacteriology Division, USAMRIID, Fort Detrick, Frederick, MD, United States of America.BioStatisitics Division, USAMRIID, Fort Detrick, Frederick, MD, United States of America.Bacteriology Division, USAMRIID, Fort Detrick, Frederick, MD, United States of America.Bacteriology Division, USAMRIID, Fort Detrick, Frederick, MD, United States of America.Bacteriology Division, USAMRIID, Fort Detrick, Frederick, MD, United States of America.Bacteriology Division, USAMRIID, Fort Detrick, Frederick, MD, United States of America.Bacteriology Division, USAMRIID, Fort Detrick, Frederick, MD, United States of America.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28235018

Citation

Bearss, Jeremy J., et al. "Characterization of Pathogenesis of and Immune Response to Burkholderia Pseudomallei K96243 Using Both Inhalational and Intraperitoneal Infection Models in BALB/c and C57BL/6 Mice." PloS One, vol. 12, no. 2, 2017, pp. e0172627.
Bearss JJ, Hunter M, Dankmeyer JL, et al. Characterization of pathogenesis of and immune response to Burkholderia pseudomallei K96243 using both inhalational and intraperitoneal infection models in BALB/c and C57BL/6 mice. PLoS One. 2017;12(2):e0172627.
Bearss, J. J., Hunter, M., Dankmeyer, J. L., Fritts, K. A., Klimko, C. P., Weaver, C. H., Shoe, J. L., Quirk, A. V., Toothman, R. G., Webster, W. M., Fetterer, D. P., Bozue, J. A., Worsham, P. L., Welkos, S. L., Amemiya, K., & Cote, C. K. (2017). Characterization of pathogenesis of and immune response to Burkholderia pseudomallei K96243 using both inhalational and intraperitoneal infection models in BALB/c and C57BL/6 mice. PloS One, 12(2), e0172627. https://doi.org/10.1371/journal.pone.0172627
Bearss JJ, et al. Characterization of Pathogenesis of and Immune Response to Burkholderia Pseudomallei K96243 Using Both Inhalational and Intraperitoneal Infection Models in BALB/c and C57BL/6 Mice. PLoS One. 2017;12(2):e0172627. PubMed PMID: 28235018.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of pathogenesis of and immune response to Burkholderia pseudomallei K96243 using both inhalational and intraperitoneal infection models in BALB/c and C57BL/6 mice. AU - Bearss,Jeremy J, AU - Hunter,Melissa, AU - Dankmeyer,Jennifer L, AU - Fritts,Kristen A, AU - Klimko,Christopher P, AU - Weaver,Chris H, AU - Shoe,Jennifer L, AU - Quirk,Avery V, AU - Toothman,Ronald G, AU - Webster,Wendy M, AU - Fetterer,David P, AU - Bozue,Joel A, AU - Worsham,Patricia L, AU - Welkos,Susan L, AU - Amemiya,Kei, AU - Cote,Christopher K, Y1 - 2017/02/24/ PY - 2016/09/29/received PY - 2017/02/07/accepted PY - 2017/2/25/entrez PY - 2017/2/25/pubmed PY - 2017/8/22/medline SP - e0172627 EP - e0172627 JF - PloS one JO - PLoS One VL - 12 IS - 2 N2 - Burkholderia pseudomallei, the etiologic agent of melioidosis, is a Gram negative bacterium designated as a Tier 1 threat. This bacterium is known to be endemic in Southeast Asia and Northern Australia and can infect humans and animals by several routes. Inhalational melioidosis has been associated with monsoonal rains in endemic areas and is also a significant concern in the biodefense community. There are currently no effective vaccines for B. pseudomallei and antibiotic treatment can be hampered by non-specific symptomology and also the high rate of naturally occurring antibiotic resistant strains. Well-characterized animal models will be essential when selecting novel medical countermeasures for evaluation prior to human clinical trials. Here, we further characterize differences between the responses of BALB/c and C57BL/6 mice when challenged with low doses of a low-passage and well-defined stock of B. pseudomallei K96243 via either intraperitoneal or aerosol routes of exposure. Before challenge, mice were implanted with a transponder to collect body temperature readings, and daily body weights were also recorded. Mice were euthanized on select days for pathological analyses and determination of the bacterial burden in selected tissues (blood, lungs, liver, and spleen). Additionally, spleen homogenate and sera samples were analyzed to better characterize the host immune response after infection with aerosolized bacteria. These clinical, pathological, and immunological data highlighted and confirmed important similarities and differences between these murine models and exposure routes. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/28235018/Characterization_of_pathogenesis_of_and_immune_response_to_Burkholderia_pseudomallei_K96243_using_both_inhalational_and_intraperitoneal_infection_models_in_BALB/c_and_C57BL/6_mice_ L2 - https://dx.plos.org/10.1371/journal.pone.0172627 DB - PRIME DP - Unbound Medicine ER -