Tags

Type your tag names separated by a space and hit enter

Chromosomal location of the fosA3 and blaCTX-M genes in Proteus mirabilis and clonal spread of Escherichia coli ST117 carrying fosA3-positive IncHI2/ST3 or F2:A-:B- plasmids in a chicken farm.
Int J Antimicrob Agents. 2017 Apr; 49(4):443-448.IJ

Abstract

The aim of this study was to investigate the spread and location of the fosA3 gene among Enterobacteriaceae from diseased broiler chickens. Twenty-nine Escherichia coli and seven Proteus mirabilis isolates recovered from one chicken farm were screened for the presence of plasmid-mediated fosfomycin resistance genes by PCR. The clonal relatedness of fosA3-positive isolates, the transferability and location of fosA3, and the genetic context of the fosA3 gene were determined. Seven P. mirabilis isolates with three different pulsed-field gel electrophoresis (PFGE) patterns and five E. coli isolates belonging to sequence type 117 (ST117) and phylogenetic group D were positive for fosA3 and all carried the blaCTX-M gene. In E. coli, the genetic structures IS26-ISEcp1-blaCTX-M-65-IS26-fosA3-1758 bp-IS26 and IS26-ISEcp1-blaCTX-M-3-blaTEM-1-IS26-fosA3-1758 bp-IS26 were present on transferable IncHI2/ST3 and F2:A-:B- plasmids, respectively. However, fosA3 was located on the chromosome of the seven P. mirabilis isolates. IS26-ISEcp1-blaCTX-M-65-IS26-fosA3-1758 bp-IS26 and IS26-blaCTX-M-14-611 bp-fosA3-1222 bp-IS26 were detected in three and four P. mirabilis isolates, respectively. Minicircles that contained both fosA3 and blaCTX-M-65 were shared between E. coli and P. mirabilis. This is the first report of the fosA3 gene integrated into the chromosome of P. mirabilis isolates with the blaCTX-M gene. The emergence and clonal spread of avian pathogenic E. coli ST117 with the feature of multidrug resistance and high virulence are a serious problem.

Authors+Show Affiliations

College of Veterinary Medicine, Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, National Reference Laboratory of Veterinary Drug Residues (SCAU), South China Agricultural University, Guangzhou, China.College of Veterinary Medicine, Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, National Reference Laboratory of Veterinary Drug Residues (SCAU), South China Agricultural University, Guangzhou, China.College of Veterinary Medicine, Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, National Reference Laboratory of Veterinary Drug Residues (SCAU), South China Agricultural University, Guangzhou, China.College of Veterinary Medicine, Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, National Reference Laboratory of Veterinary Drug Residues (SCAU), South China Agricultural University, Guangzhou, China.College of Veterinary Medicine, Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, National Reference Laboratory of Veterinary Drug Residues (SCAU), South China Agricultural University, Guangzhou, China.College of Veterinary Medicine, Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, National Reference Laboratory of Veterinary Drug Residues (SCAU), South China Agricultural University, Guangzhou, China.College of Veterinary Medicine, Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, National Reference Laboratory of Veterinary Drug Residues (SCAU), South China Agricultural University, Guangzhou, China.College of Veterinary Medicine, Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, National Reference Laboratory of Veterinary Drug Residues (SCAU), South China Agricultural University, Guangzhou, China. Electronic address: jhliu@scau.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28238801

Citation

He, Dandan, et al. "Chromosomal Location of the fosA3 and blaCTX-M Genes in Proteus Mirabilis and Clonal Spread of Escherichia Coli ST117 Carrying fosA3-positive IncHI2/ST3 or F2:A-:B- Plasmids in a Chicken Farm." International Journal of Antimicrobial Agents, vol. 49, no. 4, 2017, pp. 443-448.
He D, Liu L, Guo B, et al. Chromosomal location of the fosA3 and blaCTX-M genes in Proteus mirabilis and clonal spread of Escherichia coli ST117 carrying fosA3-positive IncHI2/ST3 or F2:A-:B- plasmids in a chicken farm. Int J Antimicrob Agents. 2017;49(4):443-448.
He, D., Liu, L., Guo, B., Wu, S., Chen, X., Wang, J., Zeng, Z., & Liu, J. H. (2017). Chromosomal location of the fosA3 and blaCTX-M genes in Proteus mirabilis and clonal spread of Escherichia coli ST117 carrying fosA3-positive IncHI2/ST3 or F2:A-:B- plasmids in a chicken farm. International Journal of Antimicrobial Agents, 49(4), 443-448. https://doi.org/10.1016/j.ijantimicag.2016.12.009
He D, et al. Chromosomal Location of the fosA3 and blaCTX-M Genes in Proteus Mirabilis and Clonal Spread of Escherichia Coli ST117 Carrying fosA3-positive IncHI2/ST3 or F2:A-:B- Plasmids in a Chicken Farm. Int J Antimicrob Agents. 2017;49(4):443-448. PubMed PMID: 28238801.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chromosomal location of the fosA3 and blaCTX-M genes in Proteus mirabilis and clonal spread of Escherichia coli ST117 carrying fosA3-positive IncHI2/ST3 or F2:A-:B- plasmids in a chicken farm. AU - He,Dandan, AU - Liu,Lanping, AU - Guo,Baowei, AU - Wu,Shengjun, AU - Chen,Xiaojie, AU - Wang,Jing, AU - Zeng,Zhenling, AU - Liu,Jian-Hua, Y1 - 2017/02/24/ PY - 2016/07/06/received PY - 2016/12/13/revised PY - 2016/12/17/accepted PY - 2017/2/28/pubmed PY - 2017/4/18/medline PY - 2017/2/28/entrez KW - CTX-M KW - Escherichia coli KW - Fosfomycin KW - Plasmid KW - Poultry KW - Proteus mirabilis SP - 443 EP - 448 JF - International journal of antimicrobial agents JO - Int J Antimicrob Agents VL - 49 IS - 4 N2 - The aim of this study was to investigate the spread and location of the fosA3 gene among Enterobacteriaceae from diseased broiler chickens. Twenty-nine Escherichia coli and seven Proteus mirabilis isolates recovered from one chicken farm were screened for the presence of plasmid-mediated fosfomycin resistance genes by PCR. The clonal relatedness of fosA3-positive isolates, the transferability and location of fosA3, and the genetic context of the fosA3 gene were determined. Seven P. mirabilis isolates with three different pulsed-field gel electrophoresis (PFGE) patterns and five E. coli isolates belonging to sequence type 117 (ST117) and phylogenetic group D were positive for fosA3 and all carried the blaCTX-M gene. In E. coli, the genetic structures IS26-ISEcp1-blaCTX-M-65-IS26-fosA3-1758 bp-IS26 and IS26-ISEcp1-blaCTX-M-3-blaTEM-1-IS26-fosA3-1758 bp-IS26 were present on transferable IncHI2/ST3 and F2:A-:B- plasmids, respectively. However, fosA3 was located on the chromosome of the seven P. mirabilis isolates. IS26-ISEcp1-blaCTX-M-65-IS26-fosA3-1758 bp-IS26 and IS26-blaCTX-M-14-611 bp-fosA3-1222 bp-IS26 were detected in three and four P. mirabilis isolates, respectively. Minicircles that contained both fosA3 and blaCTX-M-65 were shared between E. coli and P. mirabilis. This is the first report of the fosA3 gene integrated into the chromosome of P. mirabilis isolates with the blaCTX-M gene. The emergence and clonal spread of avian pathogenic E. coli ST117 with the feature of multidrug resistance and high virulence are a serious problem. SN - 1872-7913 UR - https://www.unboundmedicine.com/medline/citation/28238801/Chromosomal_location_of_the_fosA3_and_blaCTX_M_genes_in_Proteus_mirabilis_and_clonal_spread_of_Escherichia_coli_ST117_carrying_fosA3_positive_IncHI2/ST3_or_F2:A_:B__plasmids_in_a_chicken_farm_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0924-8579(17)30048-1 DB - PRIME DP - Unbound Medicine ER -