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An Improved Rodent Model of Trigeminal Neuropathic Pain by Unilateral Chronic Constriction Injury of Distal Infraorbital Nerve.
J Pain. 2017 08; 18(8):899-907.JP

Abstract

The number of studies on trigeminal nerve injury using animal models remains limited. A rodent model of trigeminal neuropathic pain was first developed in 1994, in which chronic constriction injury (CCI) is induced by ligation of the infraorbital nerve (IoN). This animal model has served as a major tool to study trigeminal neuropathic pain. Unfortunately, the surgical procedure in this model is complicated and far more difficult than ligation of peripheral nerves (eg, sciatic nerve). The aim of this study was to improve on the current surgical procedure of IoN ligation to induce trigeminal neuropathic pain in rats. We show that the IoN can be readily accessed through a small facial incision. CCI can be induced by ligation of a segment at the distal IoN (dIoN). This dIoN-CCI procedure is simple, minimally invasive, and time-saving. Our data show that the dIoN-CCI procedure consistently induced acute as well as chronic nociceptive behaviors in rats. Daily gabapentin treatment attenuated mechanical allodynia and reduced face-grooming episodes in dIoN-CCI rats.

PERSPECTIVE

The orofacial pain caused by trigeminal nerve damage is severe and perhaps more debilitating than other types of neuropathic pain. However, studies on trigeminal neuropathic pain remain limited. This is largely because of the lack of proper animal models because of the complexity of the existing surgical procedures required to induce trigeminal nerve injury. Our improved dIoN-CCI model is likely to make it more accessible to study the cellular and molecular mechanisms of neuropathic pain caused by trigeminal nerve damage.

Authors+Show Affiliations

Department of Anesthesia, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China; Hangzhou First People's Hospital, Hangzhou, China.MGH Center for Translational Pain Research, Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts.MGH Center for Translational Pain Research, Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts.MGH Center for Translational Pain Research, Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts.MGH Center for Translational Pain Research, Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts.MGH Center for Translational Pain Research, Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts.MGH Center for Translational Pain Research, Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts.Department of Anesthesia, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China. Electronic address: smzhu20088@163.com.MGH Center for Translational Pain Research, Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts. Electronic address: jmao@mgh.harvard.edu.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

28238950

Citation

Ding, Weihua, et al. "An Improved Rodent Model of Trigeminal Neuropathic Pain By Unilateral Chronic Constriction Injury of Distal Infraorbital Nerve." The Journal of Pain : Official Journal of the American Pain Society, vol. 18, no. 8, 2017, pp. 899-907.
Ding W, You Z, Shen S, et al. An Improved Rodent Model of Trigeminal Neuropathic Pain by Unilateral Chronic Constriction Injury of Distal Infraorbital Nerve. J Pain. 2017;18(8):899-907.
Ding, W., You, Z., Shen, S., Yang, J., Lim, G., Doheny, J. T., Chen, L., Zhu, S., & Mao, J. (2017). An Improved Rodent Model of Trigeminal Neuropathic Pain by Unilateral Chronic Constriction Injury of Distal Infraorbital Nerve. The Journal of Pain : Official Journal of the American Pain Society, 18(8), 899-907. https://doi.org/10.1016/j.jpain.2017.02.427
Ding W, et al. An Improved Rodent Model of Trigeminal Neuropathic Pain By Unilateral Chronic Constriction Injury of Distal Infraorbital Nerve. J Pain. 2017;18(8):899-907. PubMed PMID: 28238950.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An Improved Rodent Model of Trigeminal Neuropathic Pain by Unilateral Chronic Constriction Injury of Distal Infraorbital Nerve. AU - Ding,Weihua, AU - You,Zerong, AU - Shen,Shiqian, AU - Yang,Jinsheng, AU - Lim,Grewo, AU - Doheny,Jason T, AU - Chen,Lucy, AU - Zhu,Shengmei, AU - Mao,Jianren, Y1 - 2017/02/24/ PY - 2016/12/20/received PY - 2017/01/21/revised PY - 2017/02/01/accepted PY - 2017/2/28/pubmed PY - 2018/4/20/medline PY - 2017/2/28/entrez KW - Rat KW - chronic constriction KW - distal KW - face-grooming KW - infraorbital nerve KW - mechanical allodynia KW - orbital cavity SP - 899 EP - 907 JF - The journal of pain : official journal of the American Pain Society JO - J Pain VL - 18 IS - 8 N2 - : The number of studies on trigeminal nerve injury using animal models remains limited. A rodent model of trigeminal neuropathic pain was first developed in 1994, in which chronic constriction injury (CCI) is induced by ligation of the infraorbital nerve (IoN). This animal model has served as a major tool to study trigeminal neuropathic pain. Unfortunately, the surgical procedure in this model is complicated and far more difficult than ligation of peripheral nerves (eg, sciatic nerve). The aim of this study was to improve on the current surgical procedure of IoN ligation to induce trigeminal neuropathic pain in rats. We show that the IoN can be readily accessed through a small facial incision. CCI can be induced by ligation of a segment at the distal IoN (dIoN). This dIoN-CCI procedure is simple, minimally invasive, and time-saving. Our data show that the dIoN-CCI procedure consistently induced acute as well as chronic nociceptive behaviors in rats. Daily gabapentin treatment attenuated mechanical allodynia and reduced face-grooming episodes in dIoN-CCI rats. PERSPECTIVE: The orofacial pain caused by trigeminal nerve damage is severe and perhaps more debilitating than other types of neuropathic pain. However, studies on trigeminal neuropathic pain remain limited. This is largely because of the lack of proper animal models because of the complexity of the existing surgical procedures required to induce trigeminal nerve injury. Our improved dIoN-CCI model is likely to make it more accessible to study the cellular and molecular mechanisms of neuropathic pain caused by trigeminal nerve damage. SN - 1528-8447 UR - https://www.unboundmedicine.com/medline/citation/28238950/An_Improved_Rodent_Model_of_Trigeminal_Neuropathic_Pain_by_Unilateral_Chronic_Constriction_Injury_of_Distal_Infraorbital_Nerve_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1526-5900(17)30477-7 DB - PRIME DP - Unbound Medicine ER -