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Inhibition of microRNA-153 protects neurons against ischemia/reperfusion injury in an oxygen-glucose deprivation and reoxygenation cellular model by regulating Nrf2/HO-1 signaling.
J Biochem Mol Toxicol. 2017 Jul; 31(7)JB

Abstract

MicroRNAs are emerging as critical regulators in cerebral ischemia/reperfusion injury; however, their exact roles remain poorly understood. miR-153 is reported to be a neuron-related miRNA involved in neuroprotection. In this study, we aimed to investigate the precise role of miR-153 in regulating neuron survival during cerebral ischemia/reperfusion injury using an oxygen-glucose deprivation and reoxygenation (OGD/R) cellular model. We found that miR-153 was significantly upregulated in neurons subjected to OGD/R treatment. Inhibition of miR-153 significantly attenuated OGD/R-induced injury and oxidative stress in neurons. Nuclear factor erythroid 2-related factor 2 (Nrf2) was identified as a target gene of miR-153. Inhibition of miR-153 significantly promoted the expression of Nrf2 and heme oxygenase-1 (HO-1). However, silencing of Nrf2 significantly blocked the protective effects of miR-153 inhibition. Our study indicates that the inhibition of miR-153 protects neurons against OGD/R-induced injury by regulating Nrf2/HO-1 signaling and suggests a potential therapeutic target for cerebral ischemia/reperfusion injury.

Authors+Show Affiliations

Department of Pharmacology, College of Basic Medical Science, Jilin University, Changchun, Jilin, 130021, People's Republic of China. Department of Neonatology, Frist Hospital of Jilin University, Changchun, Jilin, 130021, People's Republic of China.Department of Pediatrics Neurology, Frist Hospital of Jilin University, Changchun, Jilin, 130021, People's Republic of China.Cadre's Ward of Frist Hospital of Jilin University, Changchun, Jilin, 130021, People's Republic of China.Department of Pharmacology, College of Basic Medical Science, Jilin University, Changchun, Jilin, 130021, People's Republic of China.Department of Pharmacology, College of Basic Medical Science, Jilin University, Changchun, Jilin, 130021, People's Republic of China.Department of Pharmacology, College of Basic Medical Science, Jilin University, Changchun, Jilin, 130021, People's Republic of China.Department of Pharmacology, College of Basic Medical Science, Jilin University, Changchun, Jilin, 130021, People's Republic of China.Department of Pharmacology, College of Basic Medical Science, Jilin University, Changchun, Jilin, 130021, People's Republic of China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28245071

Citation

Ji, Qiong, et al. "Inhibition of microRNA-153 Protects Neurons Against Ischemia/reperfusion Injury in an Oxygen-glucose Deprivation and Reoxygenation Cellular Model By Regulating Nrf2/HO-1 Signaling." Journal of Biochemical and Molecular Toxicology, vol. 31, no. 7, 2017.
Ji Q, Gao J, Zheng Y, et al. Inhibition of microRNA-153 protects neurons against ischemia/reperfusion injury in an oxygen-glucose deprivation and reoxygenation cellular model by regulating Nrf2/HO-1 signaling. J Biochem Mol Toxicol. 2017;31(7).
Ji, Q., Gao, J., Zheng, Y., Liu, X., Zhou, Q., Shi, C., Yao, M., & Chen, X. (2017). Inhibition of microRNA-153 protects neurons against ischemia/reperfusion injury in an oxygen-glucose deprivation and reoxygenation cellular model by regulating Nrf2/HO-1 signaling. Journal of Biochemical and Molecular Toxicology, 31(7). https://doi.org/10.1002/jbt.21905
Ji Q, et al. Inhibition of microRNA-153 Protects Neurons Against Ischemia/reperfusion Injury in an Oxygen-glucose Deprivation and Reoxygenation Cellular Model By Regulating Nrf2/HO-1 Signaling. J Biochem Mol Toxicol. 2017;31(7) PubMed PMID: 28245071.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibition of microRNA-153 protects neurons against ischemia/reperfusion injury in an oxygen-glucose deprivation and reoxygenation cellular model by regulating Nrf2/HO-1 signaling. AU - Ji,Qiong, AU - Gao,Jianbo, AU - Zheng,Yan, AU - Liu,Xueli, AU - Zhou,Qiangqiang, AU - Shi,Canxia, AU - Yao,Meng, AU - Chen,Xia, Y1 - 2017/02/28/ PY - 2016/12/11/received PY - 2017/01/17/revised PY - 2017/01/26/accepted PY - 2017/3/1/pubmed PY - 2017/12/22/medline PY - 2017/3/1/entrez KW - Cerebral ischemia/reperfusion injury KW - HO-1 KW - Nrf2 KW - miR-153 JF - Journal of biochemical and molecular toxicology JO - J. Biochem. Mol. Toxicol. VL - 31 IS - 7 N2 - MicroRNAs are emerging as critical regulators in cerebral ischemia/reperfusion injury; however, their exact roles remain poorly understood. miR-153 is reported to be a neuron-related miRNA involved in neuroprotection. In this study, we aimed to investigate the precise role of miR-153 in regulating neuron survival during cerebral ischemia/reperfusion injury using an oxygen-glucose deprivation and reoxygenation (OGD/R) cellular model. We found that miR-153 was significantly upregulated in neurons subjected to OGD/R treatment. Inhibition of miR-153 significantly attenuated OGD/R-induced injury and oxidative stress in neurons. Nuclear factor erythroid 2-related factor 2 (Nrf2) was identified as a target gene of miR-153. Inhibition of miR-153 significantly promoted the expression of Nrf2 and heme oxygenase-1 (HO-1). However, silencing of Nrf2 significantly blocked the protective effects of miR-153 inhibition. Our study indicates that the inhibition of miR-153 protects neurons against OGD/R-induced injury by regulating Nrf2/HO-1 signaling and suggests a potential therapeutic target for cerebral ischemia/reperfusion injury. SN - 1099-0461 UR - https://www.unboundmedicine.com/medline/citation/28245071/Inhibition_of_microRNA_153_protects_neurons_against_ischemia/reperfusion_injury_in_an_oxygen_glucose_deprivation_and_reoxygenation_cellular_model_by_regulating_Nrf2/HO_1_signaling_ L2 - https://doi.org/10.1002/jbt.21905 DB - PRIME DP - Unbound Medicine ER -