TY - JOUR T1 - Attenuation of oxidative stress and cardioprotective effects of zinc supplementation in experimental diabetic rats. AU - Barman,Susmita, AU - Srinivasan,Krishnapura, Y1 - 2017/03/01/ PY - 2017/3/2/pubmed PY - 2017/5/23/medline PY - 2017/3/2/entrez KW - 4-HNE 4-hydroxy-2-nonenal KW - Bax BCL2 associated X protein KW - Bcl-2 B-cell lymphoma 2 KW - CAT catalase KW - CK-MB creatine phosphokinase–MB KW - GPx glutathione peroxidase KW - GR glutathione reductase KW - GST glutathione-S-transferase KW - LPO lipid peroxidation KW - PAS periodic acid Schiff KW - PC protein carbonyl KW - ROS reactive oxygen species KW - SOD superoxide dismutase KW - Antioxidant enzymes KW - Cardioprotective effects KW - Diabetes mellitus KW - Fibrosis and apoptosis markers KW - Oxidative stress KW - Zinc supplementation SP - 335 EP - 350 JF - The British journal of nutrition JO - Br J Nutr VL - 117 IS - 3 N2 - Oxidative stress plays a major role in the pathogenesis of diabetes mellitus, which further exacerbates damage of cardiac, hepatic and other tissues. We have recently reported that Zn supplementation beneficially modulates hyperglycaemia and hypoinsulinaemia, with attendant reduction of associated metabolic abnormalities in diabetic rats. The present study assessed the potential of Zn supplementation in modulating oxidative stress and cardioprotective effects in diabetic rats. Diabetes was induced in Wistar rats with streptozotocin, and groups of diabetic rats were treated with 5- and 10-fold dietary Zn interventions (0·19 and 0·38 g Zn/kg diet) for 6 weeks. The markers of oxidative stress, antioxidant enzyme activities and concentrations of antioxidant molecules, lipid profile, and expressions of fibrosis and pro-apoptotic factors in the cardiac tissue were particularly assessed. Supplemental Zn showed significant attenuation of diabetes-induced oxidative stress in terms of altered antioxidant enzyme activities and increased the concentrations of antioxidant molecules. Hypercholesterolaemia and hyperlipidaemia were also significantly countered by Zn supplementation. Along with attenuated oxidative stress, Zn supplementation also showed significant cardioprotective effects by altering the mRNA expressions of fibrosis and pro-apoptotic factors (by >50 %). The expression of lipid oxidative marker 4-hydroxy-2-nonenal (4-HNE) protein in cardiac tissue of diabetic animals was rectified (68 %) by Zn supplementation. Elevated cardiac and hepatic markers in circulation and pathological abnormalities in cardiac and hepatic tissue architecture of diabetic animals were ameliorated by dietary Zn intervention. The present study indicates that Zn supplementation can attenuate diabetes-induced oxidative stress in circulation as well as in cardiac and hepatic tissues. SN - 1475-2662 UR - https://www.unboundmedicine.com/medline/citation/28245884/Attenuation_of_oxidative_stress_and_cardioprotective_effects_of_zinc_supplementation_in_experimental_diabetic_rats_ L2 - https://www.cambridge.org/core/product/identifier/S0007114517000174/type/journal_article DB - PRIME DP - Unbound Medicine ER -