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Toll-like receptor-induced expression of epithelial cytokine receptors on haemopoietic progenitors is altered in allergic asthma.
Clin Exp Allergy 2017; 47(7):900-908CE

Abstract

BACKGROUND

Haemopoietic progenitor cells (HPC) migrate to sites of allergic inflammation where, upon stimulation with epithelial cytokines, they produce Th2 cytokines and differentiate into mature eosinophils and basophils. They also express Toll-like receptors (TLR) involved in antimicrobial responses.

OBJECTIVE

The objective of this study was to compare TLR expression on peripheral blood HPC and TLR-induced responses, in particular changes in epithelial cytokine receptors, in healthy and asthmatic subjects at baseline and following allergen challenge.

METHODS

Ten healthy and 11 allergic asthmatic subjects were studied. HPC-enriched cell populations were stimulated with TLR-2, TLR-4 or TLR-9 ligands. TLR expression by circulating HPC and interleukin (IL)-25 (IL-17RB), IL-33 (ST2) and thymic stromal lymphopoietin receptor (TSLPR) expression after TLR ligation were examined by flow cytometry at baseline and, in asthmatics, following allergen challenge. The effects of dexamethasone (Dex) on TLR-induced responses were also assessed.

RESULTS

Asthmatics had significantly lower circulating HPC expressing TLR-2 and TLR-9 with a similar trend for TLR-4. TLR-4 stimulation of HPC yielded higher numbers of TSLPR+ cells in asthmatics compared with healthy subjects. A similar trend was seen for TLR-9 ligation, an effect further augmented by allergen inhalation. Allergen challenge also enhanced TLR-induced ST2 expression on HPC. Treatment with Dex in vitro increased TLR-4-induced TSLPR expression but had no effect on other epithelial cytokine receptors.

CONCLUSIONS AND CLINICAL RELEVANCE

These data demonstrate an interaction between allergen and TLR ligand exposure in asthmatics. Allergen inhalation augments the TLR-induced inflammatory response by HPC, possibly leading to increased "in situ haemopoiesis" through up-regulation of TSLPR. These findings show that HPC may be a part of the pro-inflammatory cascade in pathogen-induced asthma exacerbation through their increased responsiveness to TLR stimulation.

Authors+Show Affiliations

Department of Medicine, McMaster University, Hamilton, ON, Canada. Department of Internal Diseases, Asthma and Allergy, Medical University of Lodz, Lodz, Poland.Department of Medicine, McMaster University, Hamilton, ON, Canada.Department of Medicine, McMaster University, Hamilton, ON, Canada.Department of Medicine, McMaster University, Hamilton, ON, Canada.Department of Medicine, McMaster University, Hamilton, ON, Canada.Department of Medicine, McMaster University, Hamilton, ON, Canada.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28252235

Citation

Tworek, D, et al. "Toll-like Receptor-induced Expression of Epithelial Cytokine Receptors On Haemopoietic Progenitors Is Altered in Allergic Asthma." Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology, vol. 47, no. 7, 2017, pp. 900-908.
Tworek D, Heroux D, O'Byrne SN, et al. Toll-like receptor-induced expression of epithelial cytokine receptors on haemopoietic progenitors is altered in allergic asthma. Clin Exp Allergy. 2017;47(7):900-908.
Tworek, D., Heroux, D., O'Byrne, S. N., Mitchell, P., O'Byrne, P. M., & Denburg, J. A. (2017). Toll-like receptor-induced expression of epithelial cytokine receptors on haemopoietic progenitors is altered in allergic asthma. Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology, 47(7), pp. 900-908. doi:10.1111/cea.12913.
Tworek D, et al. Toll-like Receptor-induced Expression of Epithelial Cytokine Receptors On Haemopoietic Progenitors Is Altered in Allergic Asthma. Clin Exp Allergy. 2017;47(7):900-908. PubMed PMID: 28252235.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Toll-like receptor-induced expression of epithelial cytokine receptors on haemopoietic progenitors is altered in allergic asthma. AU - Tworek,D, AU - Heroux,D, AU - O'Byrne,S N, AU - Mitchell,P, AU - O'Byrne,P M, AU - Denburg,J A, Y1 - 2017/03/31/ PY - 2016/11/13/received PY - 2017/01/13/revised PY - 2017/02/17/accepted PY - 2017/3/3/pubmed PY - 2018/4/7/medline PY - 2017/3/3/entrez KW - TSLPR KW - allergen challenge KW - asthma KW - epithelium KW - haemopoietic progenitor cells KW - innate immunity SP - 900 EP - 908 JF - Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology JO - Clin. Exp. Allergy VL - 47 IS - 7 N2 - BACKGROUND: Haemopoietic progenitor cells (HPC) migrate to sites of allergic inflammation where, upon stimulation with epithelial cytokines, they produce Th2 cytokines and differentiate into mature eosinophils and basophils. They also express Toll-like receptors (TLR) involved in antimicrobial responses. OBJECTIVE: The objective of this study was to compare TLR expression on peripheral blood HPC and TLR-induced responses, in particular changes in epithelial cytokine receptors, in healthy and asthmatic subjects at baseline and following allergen challenge. METHODS: Ten healthy and 11 allergic asthmatic subjects were studied. HPC-enriched cell populations were stimulated with TLR-2, TLR-4 or TLR-9 ligands. TLR expression by circulating HPC and interleukin (IL)-25 (IL-17RB), IL-33 (ST2) and thymic stromal lymphopoietin receptor (TSLPR) expression after TLR ligation were examined by flow cytometry at baseline and, in asthmatics, following allergen challenge. The effects of dexamethasone (Dex) on TLR-induced responses were also assessed. RESULTS: Asthmatics had significantly lower circulating HPC expressing TLR-2 and TLR-9 with a similar trend for TLR-4. TLR-4 stimulation of HPC yielded higher numbers of TSLPR+ cells in asthmatics compared with healthy subjects. A similar trend was seen for TLR-9 ligation, an effect further augmented by allergen inhalation. Allergen challenge also enhanced TLR-induced ST2 expression on HPC. Treatment with Dex in vitro increased TLR-4-induced TSLPR expression but had no effect on other epithelial cytokine receptors. CONCLUSIONS AND CLINICAL RELEVANCE: These data demonstrate an interaction between allergen and TLR ligand exposure in asthmatics. Allergen inhalation augments the TLR-induced inflammatory response by HPC, possibly leading to increased "in situ haemopoiesis" through up-regulation of TSLPR. These findings show that HPC may be a part of the pro-inflammatory cascade in pathogen-induced asthma exacerbation through their increased responsiveness to TLR stimulation. SN - 1365-2222 UR - https://www.unboundmedicine.com/medline/citation/28252235/Toll_like_receptor_induced_expression_of_epithelial_cytokine_receptors_on_haemopoietic_progenitors_is_altered_in_allergic_asthma_ L2 - https://doi.org/10.1111/cea.12913 DB - PRIME DP - Unbound Medicine ER -