Identification of Pm58 from Aegilops tauschii.Theor Appl Genet. 2017 Jun; 130(6):1123-1133.TA
Wiersma, Andrew T., et al. "Identification of Pm58 From Aegilops Tauschii." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 130, no. 6, 2017, pp. 1123-1133.
Wiersma AT, Pulman JA, Brown LK, et al. Identification of Pm58 from Aegilops tauschii. Theor Appl Genet. 2017;130(6):1123-1133.
Wiersma, A. T., Pulman, J. A., Brown, L. K., Cowger, C., & Olson, E. L. (2017). Identification of Pm58 from Aegilops tauschii. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 130(6), 1123-1133. https://doi.org/10.1007/s00122-017-2874-8
Wiersma AT, et al. Identification of Pm58 From Aegilops Tauschii. Theor Appl Genet. 2017;130(6):1123-1133. PubMed PMID: 28255671.
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TY - JOUR T1 - Identification of Pm58 from Aegilops tauschii. AU - Wiersma,Andrew T, AU - Pulman,Jane A, AU - Brown,Linda K, AU - Cowger,Christina, AU - Olson,Eric L, Y1 - 2017/03/03/ PY - 2016/10/25/received PY - 2017/02/07/accepted PY - 2017/3/4/pubmed PY - 2017/6/21/medline PY - 2017/3/4/entrez SP - 1123 EP - 1133 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 130 IS - 6 N2 - KEY MESSAGE: A novel powdery mildew-resistance gene, designated Pm58, was introgressed directly from Aegilops tauschii to hexaploid wheat, mapped to chromosome 2DS, and confirmed to be effective under field conditions. Selectable KASP™ markers were developed for MAS. Powdery mildew caused by Blumeria graminis (DC.) f. sp. tritici (Bgt) remains a significant threat to wheat (Triticum aestivum L.) production. The rapid breakdown of race-specific resistance to Bgt reinforces the need to identify novel sources of resistance. The D-genome species, Aegilops tauschii, is an excellent source of disease resistance that is transferrable to T. aestivum. The powdery mildew-resistant Ae. tauschii accession TA1662 (2n = 2x = DD) was crossed directly with the susceptible hard white wheat line KS05HW14 (2n = 6x = AABBDD) followed by backcrossing to develop a population of 96 BC2F4 introgression lines (ILs). Genotyping-by-sequencing was used to develop a genome-wide genetic map that was anchored to the Ae. tauschii reference genome. A detached-leaf Bgt assay was used to screen BC2F4:6 ILs, and resistance was found to segregate as a single locus (χ = 2.0, P value = 0.157). The resistance gene, referred to as Pm58, mapped to chromosome 2DS. Pm58 was evaluated under field conditions in replicated trials in 2015 and 2016. In both years, a single QTL spanning the Pm58 locus was identified that reduced powdery mildew severity and explained 21% of field variation (P value < 0.01). KASP™ assays were developed from closely linked GBS-SNP markers, a refined genetic map was developed, and four markers that cosegregate with Pm58 were identified. This novel source of powdery mildew-resistance and closely linked genetic markers will support efforts to develop wheat varieties with powdery mildew resistance. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/28255671/Identification_of_Pm58_from_Aegilops_tauschii_ L2 - https://dx.doi.org/10.1007/s00122-017-2874-8 DB - PRIME DP - Unbound Medicine ER -