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Identification of Pm58 from Aegilops tauschii.
Theor Appl Genet. 2017 Jun; 130(6):1123-1133.TA

Abstract

KEY MESSAGE

A novel powdery mildew-resistance gene, designated Pm58, was introgressed directly from Aegilops tauschii to hexaploid wheat, mapped to chromosome 2DS, and confirmed to be effective under field conditions. Selectable KASP™ markers were developed for MAS. Powdery mildew caused by Blumeria graminis (DC.) f. sp. tritici (Bgt) remains a significant threat to wheat (Triticum aestivum L.) production. The rapid breakdown of race-specific resistance to Bgt reinforces the need to identify novel sources of resistance. The D-genome species, Aegilops tauschii, is an excellent source of disease resistance that is transferrable to T. aestivum. The powdery mildew-resistant Ae. tauschii accession TA1662 (2n = 2x = DD) was crossed directly with the susceptible hard white wheat line KS05HW14 (2n = 6x = AABBDD) followed by backcrossing to develop a population of 96 BC2F4 introgression lines (ILs). Genotyping-by-sequencing was used to develop a genome-wide genetic map that was anchored to the Ae. tauschii reference genome. A detached-leaf Bgt assay was used to screen BC2F4:6 ILs, and resistance was found to segregate as a single locus (χ = 2.0, P value = 0.157). The resistance gene, referred to as Pm58, mapped to chromosome 2DS. Pm58 was evaluated under field conditions in replicated trials in 2015 and 2016. In both years, a single QTL spanning the Pm58 locus was identified that reduced powdery mildew severity and explained 21% of field variation (P value < 0.01). KASP™ assays were developed from closely linked GBS-SNP markers, a refined genetic map was developed, and four markers that cosegregate with Pm58 were identified. This novel source of powdery mildew-resistance and closely linked genetic markers will support efforts to develop wheat varieties with powdery mildew resistance.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Wiersma AT
Department of Plant, Soil and Microbial Sciences, Michigan State University, 1066 Bogue Street, Room A286, East Lansing, MI, 48824, USA.
Pulman JA
Department of Plant Biology and Center for Genomics-Enabled Plant Science, Michigan State University, 612 Wilson Rd, Room 166, East Lansing, MI, 48824, USA.
Brown LK
Department of Plant, Soil and Microbial Sciences, Michigan State University, 1066 Bogue Street, Room A286, East Lansing, MI, 48824, USA.
Cowger C
Department of Plant Pathology, North Carolina State University, USDA-ARS Plant Science Research, 2510 Thomas Hall, Campus Box 7616, Raleigh, NC, 27695, USA.
Olson EL
Department of Plant, Soil and Microbial Sciences, Michigan State University, 1066 Bogue Street, Room A286, East Lansing, MI, 48824, USA. eolson@msu.edu.

MeSH

AscomycotaChromosome MappingDNA, PlantDisease ResistanceGenes, PlantGenetic LinkageGenetic MarkersGenotypeHigh-Throughput Nucleotide SequencingPlant DiseasesPoaceaeQuantitative Trait LociSequence Analysis, DNATriticum

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28255671

Citation

Wiersma, Andrew T., et al. "Identification of Pm58 From Aegilops Tauschii." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 130, no. 6, 2017, pp. 1123-1133.
Wiersma AT, Pulman JA, Brown LK, et al. Identification of Pm58 from Aegilops tauschii. Theor Appl Genet. 2017;130(6):1123-1133.
Wiersma, A. T., Pulman, J. A., Brown, L. K., Cowger, C., & Olson, E. L. (2017). Identification of Pm58 from Aegilops tauschii. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 130(6), 1123-1133. https://doi.org/10.1007/s00122-017-2874-8
Wiersma AT, et al. Identification of Pm58 From Aegilops Tauschii. Theor Appl Genet. 2017;130(6):1123-1133. PubMed PMID: 28255671.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of Pm58 from Aegilops tauschii. AU - Wiersma,Andrew T, AU - Pulman,Jane A, AU - Brown,Linda K, AU - Cowger,Christina, AU - Olson,Eric L, Y1 - 2017/03/03/ PY - 2016/10/25/received PY - 2017/02/07/accepted PY - 2017/3/4/pubmed PY - 2017/6/21/medline PY - 2017/3/4/entrez SP - 1123 EP - 1133 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 130 IS - 6 N2 - KEY MESSAGE: A novel powdery mildew-resistance gene, designated Pm58, was introgressed directly from Aegilops tauschii to hexaploid wheat, mapped to chromosome 2DS, and confirmed to be effective under field conditions. Selectable KASP™ markers were developed for MAS. Powdery mildew caused by Blumeria graminis (DC.) f. sp. tritici (Bgt) remains a significant threat to wheat (Triticum aestivum L.) production. The rapid breakdown of race-specific resistance to Bgt reinforces the need to identify novel sources of resistance. The D-genome species, Aegilops tauschii, is an excellent source of disease resistance that is transferrable to T. aestivum. The powdery mildew-resistant Ae. tauschii accession TA1662 (2n = 2x = DD) was crossed directly with the susceptible hard white wheat line KS05HW14 (2n = 6x = AABBDD) followed by backcrossing to develop a population of 96 BC2F4 introgression lines (ILs). Genotyping-by-sequencing was used to develop a genome-wide genetic map that was anchored to the Ae. tauschii reference genome. A detached-leaf Bgt assay was used to screen BC2F4:6 ILs, and resistance was found to segregate as a single locus (χ = 2.0, P value = 0.157). The resistance gene, referred to as Pm58, mapped to chromosome 2DS. Pm58 was evaluated under field conditions in replicated trials in 2015 and 2016. In both years, a single QTL spanning the Pm58 locus was identified that reduced powdery mildew severity and explained 21% of field variation (P value < 0.01). KASP™ assays were developed from closely linked GBS-SNP markers, a refined genetic map was developed, and four markers that cosegregate with Pm58 were identified. This novel source of powdery mildew-resistance and closely linked genetic markers will support efforts to develop wheat varieties with powdery mildew resistance. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/28255671/Identification_of_Pm58_from_Aegilops_tauschii_ L2 - https://dx.doi.org/10.1007/s00122-017-2874-8 DB - PRIME DP - Unbound Medicine ER -