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Molecular characterization of Orientia tsutsugamushi serotypes causing scrub typhus outbreak in southern region of Andhra Pradesh, India.
Indian J Med Res. 2016 Oct; 144(4):597-603.IJ

Abstract

BACKGROUND & OBJECTIVES

Scrub typhus is a vector-borne zoonotic infection caused by Orientiatsutsugamushi. Local epidemiology of the circulating serotypes of scrub typhus is not available from most parts of India. We conducted this study for the diagnosis of scrub typhus using IgM ELISA and to detect O. tsutsugamushi serotypes circulating in southern Andhra Pradesh, India.

METHODS

Samples were collected from patients clinically suspected to have scrub typhus and were subjected to IgM ELISA to measure IgM antibodies against O. tsutsugamushi. Nested polymerase chain reaction (PCR) was performed targeting strain-specific regions in ELISA-positive samples.

RESULTS

Of a total of 663 samples, 258 (38.91%) were found to be positive by IgM ELISA. Serotypes could be detected in 230 (34.69%) samples only. Only two serotypes, Karp and Kawasaki, were found in the serum samples, with the former being predominant. The dual infection of Karp and Kawasaki serotypes was found in seven patients. Other serotypes such as Gilliam, Kuroki and Kato were not detected in the samples.

INTERPRETATION & CONCLUSION

The nested PCR products proved useful in presumptively identifying the endemic O. tsutsugamushi serotypes. The present study could be significant in understanding scrub typhus epidemiology in this region.

Authors+Show Affiliations

Department of Virology, Sri Venkateswara University, Tirupati, India.Department of Virology, Sri Venkateswara University, Tirupati, India.Department of Microbiology, Sri Venkateswara Institute of Medical Sciences, Tirupati, India.Department of Medicine, Sri Venkateswara Institute of Medical Sciences, Tirupati, India.Department of Microbiology, Sri Venkateswara Institute of Medical Sciences, Tirupati, India.Department of Virology, Sri Venkateswara University, Tirupati, India.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28256470

Citation

Usha, K, et al. "Molecular Characterization of Orientia Tsutsugamushi Serotypes Causing Scrub Typhus Outbreak in Southern Region of Andhra Pradesh, India." The Indian Journal of Medical Research, vol. 144, no. 4, 2016, pp. 597-603.
Usha K, Kumar E, Kalawat U, et al. Molecular characterization of Orientia tsutsugamushi serotypes causing scrub typhus outbreak in southern region of Andhra Pradesh, India. Indian J Med Res. 2016;144(4):597-603.
Usha, K., Kumar, E., Kalawat, U., Kumar, B. S., Chaudhury, A., & Gopal, D. V. (2016). Molecular characterization of Orientia tsutsugamushi serotypes causing scrub typhus outbreak in southern region of Andhra Pradesh, India. The Indian Journal of Medical Research, 144(4), 597-603. https://doi.org/10.4103/0971-5916.200886
Usha K, et al. Molecular Characterization of Orientia Tsutsugamushi Serotypes Causing Scrub Typhus Outbreak in Southern Region of Andhra Pradesh, India. Indian J Med Res. 2016;144(4):597-603. PubMed PMID: 28256470.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular characterization of Orientia tsutsugamushi serotypes causing scrub typhus outbreak in southern region of Andhra Pradesh, India. AU - Usha,K, AU - Kumar,E, AU - Kalawat,Usha, AU - Kumar,B Siddhartha, AU - Chaudhury,A, AU - Gopal,D V R Sai, PY - 2017/3/4/entrez PY - 2017/3/4/pubmed PY - 2017/6/24/medline SP - 597 EP - 603 JF - The Indian journal of medical research JO - Indian J Med Res VL - 144 IS - 4 N2 - BACKGROUND & OBJECTIVES: Scrub typhus is a vector-borne zoonotic infection caused by Orientiatsutsugamushi. Local epidemiology of the circulating serotypes of scrub typhus is not available from most parts of India. We conducted this study for the diagnosis of scrub typhus using IgM ELISA and to detect O. tsutsugamushi serotypes circulating in southern Andhra Pradesh, India. METHODS: Samples were collected from patients clinically suspected to have scrub typhus and were subjected to IgM ELISA to measure IgM antibodies against O. tsutsugamushi. Nested polymerase chain reaction (PCR) was performed targeting strain-specific regions in ELISA-positive samples. RESULTS: Of a total of 663 samples, 258 (38.91%) were found to be positive by IgM ELISA. Serotypes could be detected in 230 (34.69%) samples only. Only two serotypes, Karp and Kawasaki, were found in the serum samples, with the former being predominant. The dual infection of Karp and Kawasaki serotypes was found in seven patients. Other serotypes such as Gilliam, Kuroki and Kato were not detected in the samples. INTERPRETATION & CONCLUSION: The nested PCR products proved useful in presumptively identifying the endemic O. tsutsugamushi serotypes. The present study could be significant in understanding scrub typhus epidemiology in this region. SN - 0971-5916 UR - https://www.unboundmedicine.com/medline/citation/28256470/Molecular_characterization_of_Orientia_tsutsugamushi_serotypes_causing_scrub_typhus_outbreak_in_southern_region_of_Andhra_Pradesh_India_ L2 - http://www.ijmr.org.in/article.asp?issn=0971-5916;year=2016;volume=144;issue=4;spage=597;epage=603;aulast=Usha DB - PRIME DP - Unbound Medicine ER -