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Th17/Treg imbalance and increased interleukin-21 are associated with liver injury in patients with chronic severe hepatitis B.
Int Immunopharmacol 2017; 46:48-55II

Abstract

BACKGROUND

Th17/Treg imbalance and the levels of related cytokines are essential in the pathogenesis of autoimmune and infectious diseases. The aim of the current study was to assess the Treg/Th17 balance and the levels of related cytokines associated with various degrees of liver injury in patients with chronic hepatitis B virus (HBV) infection.

METHODS

The proportions of peripheral Th17, Treg and Th1 cells in 7 patients classified as asymptomatic hepatitis B virus carriers (AsCs), 38 patients with low or moderate grade chronic hepatitis B (CHB-LM), 20 patients with chronic severe hepatitis B (CSHB), and 10 healthy controls (HCs) were determined by flow cytometry. The levels of related cytokines and the mRNA expression levels of transcription factors were measured using Enzyme-linked immunosorbent assay (ELISA) and real-time quantitative PCR (RT-PCR), respectively.

RESULTS

The Th17 cell frequency and the mRNA expression levels of RORc were increased in the CSHB group. The Treg cell frequency was increased and Th1 cell frequency and the mRNA expression levels of T-bet were decreased in chronic HBV infection. The levels of IL-21 were increased in the CSHB group and were positively correlated with AST, TB and DB in patients with chronic HBV infection. The Th17/Treg ratio was increased in the CSHB group and was positively correlated with liver injury in chronic HBV infection.

CONCLUSIONS

Th17/Treg imbalance and increased IL-21 are associated with liver injury in patients with chronic HBV infection. Restoring the Th17/Treg balance may be a novel immunotherapy for patients with CSHB.

Authors+Show Affiliations

Department of Infectious Diseases, Institute for Viral Hepatitis, Key Laboratory of Molecular Biology for Infectious Diseases, Ministry of Education, Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.Department of Infectious Diseases, Institute for Viral Hepatitis, Key Laboratory of Molecular Biology for Infectious Diseases, Ministry of Education, Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.Department of Infectious Diseases, Institute for Viral Hepatitis, Key Laboratory of Molecular Biology for Infectious Diseases, Ministry of Education, Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.Department of Infectious Diseases, Institute for Viral Hepatitis, Key Laboratory of Molecular Biology for Infectious Diseases, Ministry of Education, Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.Department of Infectious Diseases, Institute for Viral Hepatitis, Key Laboratory of Molecular Biology for Infectious Diseases, Ministry of Education, Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.Department of Infectious Diseases, Institute for Viral Hepatitis, Key Laboratory of Molecular Biology for Infectious Diseases, Ministry of Education, Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.Department of Infectious Diseases, Institute for Viral Hepatitis, Key Laboratory of Molecular Biology for Infectious Diseases, Ministry of Education, Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.Department of Infectious Diseases, Institute for Viral Hepatitis, Key Laboratory of Molecular Biology for Infectious Diseases, Ministry of Education, Second Affiliated Hospital of Chongqing Medical University, Chongqing, China. Electronic address: hp_cq@163.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28259000

Citation

Liu, Bin, et al. "Th17/Treg Imbalance and Increased Interleukin-21 Are Associated With Liver Injury in Patients With Chronic Severe Hepatitis B." International Immunopharmacology, vol. 46, 2017, pp. 48-55.
Liu B, Gao W, Zhang L, et al. Th17/Treg imbalance and increased interleukin-21 are associated with liver injury in patients with chronic severe hepatitis B. Int Immunopharmacol. 2017;46:48-55.
Liu, B., Gao, W., Zhang, L., Wang, J., Chen, M., Peng, M., ... Hu, P. (2017). Th17/Treg imbalance and increased interleukin-21 are associated with liver injury in patients with chronic severe hepatitis B. International Immunopharmacology, 46, pp. 48-55. doi:10.1016/j.intimp.2017.02.019.
Liu B, et al. Th17/Treg Imbalance and Increased Interleukin-21 Are Associated With Liver Injury in Patients With Chronic Severe Hepatitis B. Int Immunopharmacol. 2017;46:48-55. PubMed PMID: 28259000.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Th17/Treg imbalance and increased interleukin-21 are associated with liver injury in patients with chronic severe hepatitis B. AU - Liu,Bin, AU - Gao,Wei, AU - Zhang,Li, AU - Wang,Jing, AU - Chen,Min, AU - Peng,Mingli, AU - Ren,Hong, AU - Hu,Peng, Y1 - 2017/03/01/ PY - 2016/10/18/received PY - 2017/01/24/revised PY - 2017/02/17/accepted PY - 2017/3/5/pubmed PY - 2017/6/21/medline PY - 2017/3/5/entrez KW - Chronic hepatitis B KW - Cytokines KW - Th1 cell KW - Th17 cell KW - Treg cell SP - 48 EP - 55 JF - International immunopharmacology JO - Int. Immunopharmacol. VL - 46 N2 - BACKGROUND: Th17/Treg imbalance and the levels of related cytokines are essential in the pathogenesis of autoimmune and infectious diseases. The aim of the current study was to assess the Treg/Th17 balance and the levels of related cytokines associated with various degrees of liver injury in patients with chronic hepatitis B virus (HBV) infection. METHODS: The proportions of peripheral Th17, Treg and Th1 cells in 7 patients classified as asymptomatic hepatitis B virus carriers (AsCs), 38 patients with low or moderate grade chronic hepatitis B (CHB-LM), 20 patients with chronic severe hepatitis B (CSHB), and 10 healthy controls (HCs) were determined by flow cytometry. The levels of related cytokines and the mRNA expression levels of transcription factors were measured using Enzyme-linked immunosorbent assay (ELISA) and real-time quantitative PCR (RT-PCR), respectively. RESULTS: The Th17 cell frequency and the mRNA expression levels of RORc were increased in the CSHB group. The Treg cell frequency was increased and Th1 cell frequency and the mRNA expression levels of T-bet were decreased in chronic HBV infection. The levels of IL-21 were increased in the CSHB group and were positively correlated with AST, TB and DB in patients with chronic HBV infection. The Th17/Treg ratio was increased in the CSHB group and was positively correlated with liver injury in chronic HBV infection. CONCLUSIONS: Th17/Treg imbalance and increased IL-21 are associated with liver injury in patients with chronic HBV infection. Restoring the Th17/Treg balance may be a novel immunotherapy for patients with CSHB. SN - 1878-1705 UR - https://www.unboundmedicine.com/medline/citation/28259000/Th17/Treg_imbalance_and_increased_interleukin_21_are_associated_with_liver_injury_in_patients_with_chronic_severe_hepatitis_B_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1567-5769(17)30074-7 DB - PRIME DP - Unbound Medicine ER -