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[Epidemiological investigation of two leptospirosis death cases in Guizhou Province].
Zhonghua Yu Fang Yi Xue Za Zhi 2017; 51(3):243-247ZY

Abstract

Objective:

To conduct an epidemiological investigation of two leptospirosis death cases reported in Guizhou Province in 2014.

Methods:

The information of the patients were investigated and analyzed. The serological detection, samples of the two patients was detected using ELISA and microscopic agglutination test (MAT). Leptospira carrier status of murine host animal in the living environment of the two patients was investigated in October and November of 2014. Leptospires in the kidney were cultured and isolated, the isolates were identified using Leptospira specific PCR and further identified with serogroup specific PCR and the conventional MAT. The relativity between the carrier status of murine and the death cases of human leptospirosis was analyzed.

Results:

The two death cases of human leptospirosis came from Liping County and the clinical symptoms were consistent with the diagnosis criteria for Leptospirosis. The results of ELISA detection showed that the anti-Leptospira antibody was positive for one of the death cases, MAT identified the serum reacted with sera-group icterohaemorrhagiae Leptospira, while the serum sample of the other case was failed to perform antibody detection due to hemolysis. 1 600 traps were placed in the living environment of the two death cases and 183 murine rodents were trapped. The murine density was 11.44% (183/1 600); 40 leptospirea suspected strains were isolated and all of them were isolated from Apodemus agrarius. The positive rate was 21.86% (40/183); 95 Apodemus agrarius were trapped and the murine density was 5.93% (95/1 600). Species specific PCR identified all the 40 strains as Leptospire. Serogroup specific PCR further identification showed that they were iterohaemorrahgiae serogroup Leptospria. interrogans.

Conclusion:

Anti-iterohaemorrahgiae Leptospira antibody was detected from one of the two patients. 40 strains of iterohaemorrahgiae serogroup Leptospira interrogans were isolated and all of them were isolated from Apodemus agrarius in the living environment and the serogroup of the Leptospira matched with the serological detection results from patients, which indicated that the two death cases were caused by the infection of iterohaemorrahgiae serogroup Leptospira interrogans, and Apodemus agrarius were the potential source of infection.

Authors+Show Affiliations

Institute for Communicable Disease Control and Prevention, Guizhou Provincial Center for Disease Control and Prevention, Guiyang 550004, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Case Reports
Journal Article

Language

chi

PubMed ID

28260339

Citation

Liu, Y, et al. "[Epidemiological Investigation of Two Leptospirosis Death Cases in Guizhou Province]." Zhonghua Yu Fang Yi Xue Za Zhi [Chinese Journal of Preventive Medicine], vol. 51, no. 3, 2017, pp. 243-247.
Liu Y, Li SJ, Yao GH, et al. [Epidemiological investigation of two leptospirosis death cases in Guizhou Province]. Zhonghua Yu Fang Yi Xue Za Zhi. 2017;51(3):243-247.
Liu, Y., Li, S. J., Yao, G. H., Huang, H., Ma, Q., Zhou, J. Z., ... Wang, D. M. (2017). [Epidemiological investigation of two leptospirosis death cases in Guizhou Province]. Zhonghua Yu Fang Yi Xue Za Zhi [Chinese Journal of Preventive Medicine], 51(3), pp. 243-247. doi:10.3760/cma.j.issn.0253-9624.2017.03.010.
Liu Y, et al. [Epidemiological Investigation of Two Leptospirosis Death Cases in Guizhou Province]. Zhonghua Yu Fang Yi Xue Za Zhi. 2017 Mar 6;51(3):243-247. PubMed PMID: 28260339.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Epidemiological investigation of two leptospirosis death cases in Guizhou Province]. AU - Liu,Y, AU - Li,S J, AU - Yao,G H, AU - Huang,H, AU - Ma,Q, AU - Zhou,J Z, AU - Tang,G P, AU - Wang,D M, PY - 2017/3/6/entrez PY - 2017/3/6/pubmed PY - 2017/10/24/medline KW - Death cases KW - Host animals KW - Leptospirosis KW - Serology SP - 243 EP - 247 JF - Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine] JO - Zhonghua Yu Fang Yi Xue Za Zhi VL - 51 IS - 3 N2 - Objective: To conduct an epidemiological investigation of two leptospirosis death cases reported in Guizhou Province in 2014. Methods: The information of the patients were investigated and analyzed. The serological detection, samples of the two patients was detected using ELISA and microscopic agglutination test (MAT). Leptospira carrier status of murine host animal in the living environment of the two patients was investigated in October and November of 2014. Leptospires in the kidney were cultured and isolated, the isolates were identified using Leptospira specific PCR and further identified with serogroup specific PCR and the conventional MAT. The relativity between the carrier status of murine and the death cases of human leptospirosis was analyzed. Results: The two death cases of human leptospirosis came from Liping County and the clinical symptoms were consistent with the diagnosis criteria for Leptospirosis. The results of ELISA detection showed that the anti-Leptospira antibody was positive for one of the death cases, MAT identified the serum reacted with sera-group icterohaemorrhagiae Leptospira, while the serum sample of the other case was failed to perform antibody detection due to hemolysis. 1 600 traps were placed in the living environment of the two death cases and 183 murine rodents were trapped. The murine density was 11.44% (183/1 600); 40 leptospirea suspected strains were isolated and all of them were isolated from Apodemus agrarius. The positive rate was 21.86% (40/183); 95 Apodemus agrarius were trapped and the murine density was 5.93% (95/1 600). Species specific PCR identified all the 40 strains as Leptospire. Serogroup specific PCR further identification showed that they were iterohaemorrahgiae serogroup Leptospria. interrogans. Conclusion: Anti-iterohaemorrahgiae Leptospira antibody was detected from one of the two patients. 40 strains of iterohaemorrahgiae serogroup Leptospira interrogans were isolated and all of them were isolated from Apodemus agrarius in the living environment and the serogroup of the Leptospira matched with the serological detection results from patients, which indicated that the two death cases were caused by the infection of iterohaemorrahgiae serogroup Leptospira interrogans, and Apodemus agrarius were the potential source of infection. SN - 0253-9624 UR - https://www.unboundmedicine.com/medline/citation/28260339/[Epidemiological_investigation_of_two_leptospirosis_death_cases_in_Guizhou_Province]_ L2 - http://journal.yiigle.com/LinkIn.do?linkin_type=pubmed&issn=0253-9624&year=2017&vol=51&issue=3&fpage=243 DB - PRIME DP - Unbound Medicine ER -