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Glyoxylate bypass operon of Escherichia coli: cloning and determination of the functional map.
J Bacteriol. 1988 Jan; 170(1):386-92.JB

Abstract

In Escherichia coli, a single operon encodes the metabolic and regulatory enzymes of the glyoxylate bypass. The metabolic enzymes, isocitrate lyase and malate synthase, are expressed from aceA and aceB, and the regulatory enzyme, isocitrate dehydrogenase kinase/phosphatase, is expressed from aceK. We cloned this operon and determined its functional map by deletion analysis. The order of the genes in this operon is aceB-aceA-aceK, with aceB proximal to the promoter, consistent with the results of previous experiments using genetic techniques. The promoter was identified by S1 nuclease mapping, and its nucleotide sequence was determined. Isocitrate lyase and malate synthase were readily identified by autoradiography after the products of the operon clone were labeled by the maxicell procedure and then resolved by electrophoresis. In contrast, isocitrate dehydrogenase kinase/phosphatase, expressed from the same plasmid, was undetectable. This observation is consistent with a striking downshift in expression between aceA and aceK.

Authors+Show Affiliations

Department of Biochemistry, University of Minnesota, Minneapolis 55455.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

2826399

Citation

Chung, T, et al. "Glyoxylate Bypass Operon of Escherichia Coli: Cloning and Determination of the Functional Map." Journal of Bacteriology, vol. 170, no. 1, 1988, pp. 386-92.
Chung T, Klumpp DJ, LaPorte DC. Glyoxylate bypass operon of Escherichia coli: cloning and determination of the functional map. J Bacteriol. 1988;170(1):386-92.
Chung, T., Klumpp, D. J., & LaPorte, D. C. (1988). Glyoxylate bypass operon of Escherichia coli: cloning and determination of the functional map. Journal of Bacteriology, 170(1), 386-92.
Chung T, Klumpp DJ, LaPorte DC. Glyoxylate Bypass Operon of Escherichia Coli: Cloning and Determination of the Functional Map. J Bacteriol. 1988;170(1):386-92. PubMed PMID: 2826399.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Glyoxylate bypass operon of Escherichia coli: cloning and determination of the functional map. AU - Chung,T, AU - Klumpp,D J, AU - LaPorte,D C, PY - 1988/1/1/pubmed PY - 1988/1/1/medline PY - 1988/1/1/entrez SP - 386 EP - 92 JF - Journal of bacteriology JO - J Bacteriol VL - 170 IS - 1 N2 - In Escherichia coli, a single operon encodes the metabolic and regulatory enzymes of the glyoxylate bypass. The metabolic enzymes, isocitrate lyase and malate synthase, are expressed from aceA and aceB, and the regulatory enzyme, isocitrate dehydrogenase kinase/phosphatase, is expressed from aceK. We cloned this operon and determined its functional map by deletion analysis. The order of the genes in this operon is aceB-aceA-aceK, with aceB proximal to the promoter, consistent with the results of previous experiments using genetic techniques. The promoter was identified by S1 nuclease mapping, and its nucleotide sequence was determined. Isocitrate lyase and malate synthase were readily identified by autoradiography after the products of the operon clone were labeled by the maxicell procedure and then resolved by electrophoresis. In contrast, isocitrate dehydrogenase kinase/phosphatase, expressed from the same plasmid, was undetectable. This observation is consistent with a striking downshift in expression between aceA and aceK. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/2826399/Glyoxylate_bypass_operon_of_Escherichia_coli:_cloning_and_determination_of_the_functional_map_ DB - PRIME DP - Unbound Medicine ER -