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Nucleotide sequence and expression of the aceK gene coding for isocitrate dehydrogenase kinase/phosphatase in Escherichia coli.
J Bacteriol. 1988 Jan; 170(1):89-97.JB

Abstract

The flow of isocitrate through the glyoxylate bypass in Escherichia coli is regulated via the phosphorylation-dephosphorylation of isocitrate dehydrogenase mediated by a bifunctional enzyme: isocitrate dehydrogenase kinase/phosphatase. The aceK gene coding for this enzyme is part of the polycistronic ace operon, which also includes the aceB and aceA genes coding, respectively, for malate synthase and isocitrate lyase, the two glyoxylate bypass enzymes. The complete nucleotide sequence of a 2,214-base-pair DNA fragment containing the aceK gene and its 5' flanking region has been determined. In vivo experiments based on gene expression in a minicell system and protein fusion with beta-galactosidase, as well as in vitro assays with a plasmid-directed transcription-translation coupled system, have shown that the aceK gene extends over 1,731 nucleotides encoding a 66,528-dalton protein. The 5' flanking region presents an unusual intercistronic structural pattern consisting of two consecutive long dyad symmetries, almost identical in sequence, which can yield very stable stem-loop units. These structures are probably responsible for the drastic downshifting in expression observed in acetate-grown bacteria between the aceK gene and the aceA gene located immediately upstream in the ace operon.

Authors+Show Affiliations

Laboratory of Molecular Biology, University of Lyon, Villeurbanne, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

2826408

Citation

Cortay, J C., et al. "Nucleotide Sequence and Expression of the aceK Gene Coding for Isocitrate Dehydrogenase Kinase/phosphatase in Escherichia Coli." Journal of Bacteriology, vol. 170, no. 1, 1988, pp. 89-97.
Cortay JC, Bleicher F, Rieul C, et al. Nucleotide sequence and expression of the aceK gene coding for isocitrate dehydrogenase kinase/phosphatase in Escherichia coli. J Bacteriol. 1988;170(1):89-97.
Cortay, J. C., Bleicher, F., Rieul, C., Reeves, H. C., & Cozzone, A. J. (1988). Nucleotide sequence and expression of the aceK gene coding for isocitrate dehydrogenase kinase/phosphatase in Escherichia coli. Journal of Bacteriology, 170(1), 89-97.
Cortay JC, et al. Nucleotide Sequence and Expression of the aceK Gene Coding for Isocitrate Dehydrogenase Kinase/phosphatase in Escherichia Coli. J Bacteriol. 1988;170(1):89-97. PubMed PMID: 2826408.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nucleotide sequence and expression of the aceK gene coding for isocitrate dehydrogenase kinase/phosphatase in Escherichia coli. AU - Cortay,J C, AU - Bleicher,F, AU - Rieul,C, AU - Reeves,H C, AU - Cozzone,A J, PY - 1988/1/1/pubmed PY - 1988/1/1/medline PY - 1988/1/1/entrez SP - 89 EP - 97 JF - Journal of bacteriology JO - J Bacteriol VL - 170 IS - 1 N2 - The flow of isocitrate through the glyoxylate bypass in Escherichia coli is regulated via the phosphorylation-dephosphorylation of isocitrate dehydrogenase mediated by a bifunctional enzyme: isocitrate dehydrogenase kinase/phosphatase. The aceK gene coding for this enzyme is part of the polycistronic ace operon, which also includes the aceB and aceA genes coding, respectively, for malate synthase and isocitrate lyase, the two glyoxylate bypass enzymes. The complete nucleotide sequence of a 2,214-base-pair DNA fragment containing the aceK gene and its 5' flanking region has been determined. In vivo experiments based on gene expression in a minicell system and protein fusion with beta-galactosidase, as well as in vitro assays with a plasmid-directed transcription-translation coupled system, have shown that the aceK gene extends over 1,731 nucleotides encoding a 66,528-dalton protein. The 5' flanking region presents an unusual intercistronic structural pattern consisting of two consecutive long dyad symmetries, almost identical in sequence, which can yield very stable stem-loop units. These structures are probably responsible for the drastic downshifting in expression observed in acetate-grown bacteria between the aceK gene and the aceA gene located immediately upstream in the ace operon. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/2826408/Nucleotide_sequence_and_expression_of_the_aceK_gene_coding_for_isocitrate_dehydrogenase_kinase/phosphatase_in_Escherichia_coli_ DB - PRIME DP - Unbound Medicine ER -