Tags

Type your tag names separated by a space and hit enter

Zinc-induced differential oxidative stress and antioxidant responses in Chlorella sorokiniana and Scenedesmus acuminatus.
Ecotoxicol Environ Saf. 2017 Jun; 140:256-263.EE

Abstract

Algae are frequently exposed to toxic metals, and zinc (Zn) is one of the major toxicants present. We exposed two green microalgae, Chlorella sorokiniana and Scenedesmus acuminatus, to sub-lethal concentrations (1.0 and 0.6mM) of Zn for seven days. Algal responses were analysed at the level of growth, oxidative stress, and antioxidants. Growth parameters such as cell culture yield and pigment content were less affected by Zn in C. sorokiniana, despite the fact that this alga accumulated more zinc than S. acuminatus. Also, C. sorokiniana, but not S. acuminatus, was able to acclimatize during long-term exposure to toxic concentrations of the test metals (specific growth rate (µ) was 0.041/day and total chlorophyll was 14.6mg/mL). Although, Zn induced oxidative stress in both species, C. sorokiniana experienced less stress than S. acuminatus. This could be explained by a higher accumulation of antioxidants in C. sorokiniana, where flavonoids, polyphenols, tocopherols, glutathione (GSH) and ascorbate (ASC) content increased. Moreover, antioxidant enzymes glutathione S transferase (GST), glutathione reductase (GR), superoxide dismutase (SOD), peroxidase (POX) and ascorbate peroxidase (APX), showed increased activities in C. sorokiniana. In addition to, and probably also underlying, the higher Zn tolerance in C. sorokiniana, this alga also showed higher Zn biosorption capacity. Use of C. sorokiniana as a bio-remediator, could be considered.

Links

Publisher Full Text

Authors+Show Affiliations

Hamed SM
Department of Soil Microbiology, Soils Water and Environment Research Institute, Agricultural Research Center, Giza, Egypt. Electronic address: seham_moussa939@yahoo.com.
Zinta G
Integrated Molecular Plant Physiology Research, Department of Biology, University of Antwerp, Antwerp, Belgium; Centre of Excellence PLECO (Plant and Vegetation Ecology), Department of Biology, University of Antwerp, Antwerp, Belgium.
Klöck G
Hochschule Bremen, University of Applied Sciences, Bremen, Germany.
Asard H
Integrated Molecular Plant Physiology Research, Department of Biology, University of Antwerp, Antwerp, Belgium.
Selim S
Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Aljouf University, Sakaka, P.O. 2014, Saudi Arabia; Microbiology and Botany Department, Faculty of Science, Suez Canal University, Ismailia, P.O.Box 41522, Egypt.
AbdElgawad H
Integrated Molecular Plant Physiology Research, Department of Biology, University of Antwerp, Antwerp, Belgium; Department of Botany & Microbiology, Faculty of Science, University of Beni-Suef, Beni-Suef 62511, Egypt. Electronic address: hamada.abdelgawad@uantwerpen.be.

MeSH

AntioxidantsAscorbate PeroxidasesAscorbic AcidChlorellaChlorophyllGlutathioneGlutathione ReductaseMetals, HeavyOxidative StressOxidoreductasesPeroxidasePeroxidasesScenedesmusSuperoxide DismutaseZinc

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28273625

Citation

Hamed, Seham M., et al. "Zinc-induced Differential Oxidative Stress and Antioxidant Responses in Chlorella Sorokiniana and Scenedesmus Acuminatus." Ecotoxicology and Environmental Safety, vol. 140, 2017, pp. 256-263.
Hamed SM, Zinta G, Klöck G, et al. Zinc-induced differential oxidative stress and antioxidant responses in Chlorella sorokiniana and Scenedesmus acuminatus. Ecotoxicol Environ Saf. 2017;140:256-263.
Hamed, S. M., Zinta, G., Klöck, G., Asard, H., Selim, S., & AbdElgawad, H. (2017). Zinc-induced differential oxidative stress and antioxidant responses in Chlorella sorokiniana and Scenedesmus acuminatus. Ecotoxicology and Environmental Safety, 140, 256-263. https://doi.org/10.1016/j.ecoenv.2017.02.055
Hamed SM, et al. Zinc-induced Differential Oxidative Stress and Antioxidant Responses in Chlorella Sorokiniana and Scenedesmus Acuminatus. Ecotoxicol Environ Saf. 2017;140:256-263. PubMed PMID: 28273625.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Zinc-induced differential oxidative stress and antioxidant responses in Chlorella sorokiniana and Scenedesmus acuminatus. AU - Hamed,Seham M, AU - Zinta,Gaurav, AU - Klöck,Gerd, AU - Asard,Han, AU - Selim,Samy, AU - AbdElgawad,Hamada, Y1 - 2017/03/08/ PY - 2016/07/13/received PY - 2017/02/28/revised PY - 2017/02/28/accepted PY - 2017/3/9/pubmed PY - 2017/6/1/medline PY - 2017/3/9/entrez KW - Antioxidants KW - Green microalgae KW - Oxidative stress KW - Zinc KW - Zinc detoxification SP - 256 EP - 263 JF - Ecotoxicology and environmental safety JO - Ecotoxicol Environ Saf VL - 140 N2 - Algae are frequently exposed to toxic metals, and zinc (Zn) is one of the major toxicants present. We exposed two green microalgae, Chlorella sorokiniana and Scenedesmus acuminatus, to sub-lethal concentrations (1.0 and 0.6mM) of Zn for seven days. Algal responses were analysed at the level of growth, oxidative stress, and antioxidants. Growth parameters such as cell culture yield and pigment content were less affected by Zn in C. sorokiniana, despite the fact that this alga accumulated more zinc than S. acuminatus. Also, C. sorokiniana, but not S. acuminatus, was able to acclimatize during long-term exposure to toxic concentrations of the test metals (specific growth rate (µ) was 0.041/day and total chlorophyll was 14.6mg/mL). Although, Zn induced oxidative stress in both species, C. sorokiniana experienced less stress than S. acuminatus. This could be explained by a higher accumulation of antioxidants in C. sorokiniana, where flavonoids, polyphenols, tocopherols, glutathione (GSH) and ascorbate (ASC) content increased. Moreover, antioxidant enzymes glutathione S transferase (GST), glutathione reductase (GR), superoxide dismutase (SOD), peroxidase (POX) and ascorbate peroxidase (APX), showed increased activities in C. sorokiniana. In addition to, and probably also underlying, the higher Zn tolerance in C. sorokiniana, this alga also showed higher Zn biosorption capacity. Use of C. sorokiniana as a bio-remediator, could be considered. SN - 1090-2414 UR - https://www.unboundmedicine.com/medline/citation/28273625/Zinc_induced_differential_oxidative_stress_and_antioxidant_responses_in_Chlorella_sorokiniana_and_Scenedesmus_acuminatus_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0147-6513(17)30133-1 DB - PRIME DP - Unbound Medicine ER -