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Developmental and reproductive toxicity of PVP/PEI-coated silver nanoparticles to zebrafish.

Abstract

Cellular and molecular mechanisms of toxicity of silver nanoparticles (NPs) and their toxicity to fish embryos after waterborne exposure have been widely investigated, but much less information is available regarding the effect of Ag NPs on physiological functions such as growth or reproduction. In this work, the effects of waterborne exposure of adult zebrafish (Danio rerio) to PVP/PEI coated Ag NPs (~5nm) on reproduction (fecundity) were investigated. Moreover, the development of the embryos after parental exposure was compared with the development of embryos after direct waterborne exposure to the NPs. For this, two experiments were run: 1) embryos from unexposed parents were treated for 5days with Ag NPs (10μgAgL-1-10mgAgL-1) and development was monitored, and 2) selected breeding zebrafish were exposed for 3weeks to 100ngAgL-1 (environmentally relevant concentration) or to 10μgAgL-1 of Ag NPs, fecundity was scored and development of resulting embryos was monitored up to 5days. Waterborne exposure of embryos to Ag NPs resulted in being highly toxic (LC50 at 120h=50μgAgL-1), causing 100% mortality during the first 24h of exposure at 0.1mgAgL-1. Exposure of adults, even at the environmentally relevant silver concentration, caused a significant reduction of fecundity by the second week of treatment and resulting embryos showed a higher prevalence of malformations than control embryos. Exposed adult females presented higher prevalence of vacuolization in the liver. These results show that Ag NPs at an environmentally relevant concentration are able to affect population level parameters in zebrafish.

Authors+Show Affiliations

CBET Research Group, Dept. of Zoology and Animal Cell Biology, Research Centre for Experimental Marine Biology and Biotechnology PIE and Science and Technology Faculty, University of Basque Country (UPV/EHU), Sarriena z/g, E-48940 Leioa, Basque Country, Spain. Electronic address: amaia.orbea@ehu.eus.CBET Research Group, Dept. of Zoology and Animal Cell Biology, Research Centre for Experimental Marine Biology and Biotechnology PIE and Science and Technology Faculty, University of Basque Country (UPV/EHU), Sarriena z/g, E-48940 Leioa, Basque Country, Spain.CBET Research Group, Dept. of Zoology and Animal Cell Biology, Research Centre for Experimental Marine Biology and Biotechnology PIE and Science and Technology Faculty, University of Basque Country (UPV/EHU), Sarriena z/g, E-48940 Leioa, Basque Country, Spain.Dept. of Applied Mathematics, Statistics and Operations Research, Science and Technology Faculty, University of the Basque Country UPV/EHU, Leioa, Spain.CBET Research Group, Dept. of Zoology and Animal Cell Biology, Research Centre for Experimental Marine Biology and Biotechnology PIE and Science and Technology Faculty, University of Basque Country (UPV/EHU), Sarriena z/g, E-48940 Leioa, Basque Country, Spain.

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

28274763

Citation

Orbea, Amaia, et al. "Developmental and Reproductive Toxicity of PVP/PEI-coated Silver Nanoparticles to Zebrafish." Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP, vol. 199, 2017, pp. 59-68.
Orbea A, González-Soto N, Lacave JM, et al. Developmental and reproductive toxicity of PVP/PEI-coated silver nanoparticles to zebrafish. Comp Biochem Physiol C Toxicol Pharmacol. 2017;199:59-68.
Orbea, A., González-Soto, N., Lacave, J. M., Barrio, I., & Cajaraville, M. P. (2017). Developmental and reproductive toxicity of PVP/PEI-coated silver nanoparticles to zebrafish. Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP, 199, 59-68. https://doi.org/10.1016/j.cbpc.2017.03.004
Orbea A, et al. Developmental and Reproductive Toxicity of PVP/PEI-coated Silver Nanoparticles to Zebrafish. Comp Biochem Physiol C Toxicol Pharmacol. 2017;199:59-68. PubMed PMID: 28274763.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Developmental and reproductive toxicity of PVP/PEI-coated silver nanoparticles to zebrafish. AU - Orbea,Amaia, AU - González-Soto,Nagore, AU - Lacave,José María, AU - Barrio,Irantzu, AU - Cajaraville,Miren P, Y1 - 2017/03/06/ PY - 2016/11/30/received PY - 2017/02/23/revised PY - 2017/03/02/accepted PY - 2017/3/10/pubmed PY - 2017/8/10/medline PY - 2017/3/10/entrez KW - Developmental malformations KW - Embryotoxicity KW - Fecundity KW - Reproduction KW - Silver nanoparticles KW - Zebrafish SP - 59 EP - 68 JF - Comparative biochemistry and physiology. Toxicology & pharmacology : CBP JO - Comp Biochem Physiol C Toxicol Pharmacol VL - 199 N2 - Cellular and molecular mechanisms of toxicity of silver nanoparticles (NPs) and their toxicity to fish embryos after waterborne exposure have been widely investigated, but much less information is available regarding the effect of Ag NPs on physiological functions such as growth or reproduction. In this work, the effects of waterborne exposure of adult zebrafish (Danio rerio) to PVP/PEI coated Ag NPs (~5nm) on reproduction (fecundity) were investigated. Moreover, the development of the embryos after parental exposure was compared with the development of embryos after direct waterborne exposure to the NPs. For this, two experiments were run: 1) embryos from unexposed parents were treated for 5days with Ag NPs (10μgAgL-1-10mgAgL-1) and development was monitored, and 2) selected breeding zebrafish were exposed for 3weeks to 100ngAgL-1 (environmentally relevant concentration) or to 10μgAgL-1 of Ag NPs, fecundity was scored and development of resulting embryos was monitored up to 5days. Waterborne exposure of embryos to Ag NPs resulted in being highly toxic (LC50 at 120h=50μgAgL-1), causing 100% mortality during the first 24h of exposure at 0.1mgAgL-1. Exposure of adults, even at the environmentally relevant silver concentration, caused a significant reduction of fecundity by the second week of treatment and resulting embryos showed a higher prevalence of malformations than control embryos. Exposed adult females presented higher prevalence of vacuolization in the liver. These results show that Ag NPs at an environmentally relevant concentration are able to affect population level parameters in zebrafish. SN - 1532-0456 UR - https://www.unboundmedicine.com/medline/citation/28274763/Developmental_and_reproductive_toxicity_of_PVP/PEI_coated_silver_nanoparticles_to_zebrafish_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1532-0456(17)30057-1 DB - PRIME DP - Unbound Medicine ER -