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Bioactivity-guided isolation of flavonoids from Cynanchum acutum L. subsp. sibiricum (willd.) Rech. f. and investigation of their antiproliferative activity.
Nat Prod Res. 2017 Nov; 31(22):2629-2633.NP

Abstract

Cynanchum acutum L. subsp. sibiricum (Willd.) Rech. f. was extracted with hexane, acetone, methanol and water individually. A sample was heated in water then extracted with ethyl acetate. Among the extracts, the ethyl acetate extract exhibited the most antiproliferative activity, so isolation of bioactive compounds was carried out from this extract. A new compound, kaempferol-3-O-β-xylopyranosyl-(1-2)-β-rhamnopyranoside (1) along with five known compounds, quercetin-3-O-β-xyloside (2), kaempferol-3-O-β-glucoside (3), quercetin-3-O-β-glucoside (4), kaempferol-3-O-β-rhamnopyranoside (5), and kaempferol-3-O-β-d-neohesperidoside (6) were isolated from ethyl acetate extract. The structures were elucidated by spectroscopic techniques, basically 1D NMR, 2D NMR and LC-TOF/MS. Antiproliferative effects of isolated compounds were determined by xCELLigence using the HeLa (human uterus carcinoma) cell lines. Compound 2 and compound 5 revealed the good antiproliferative activity against HeLa cell lines.

Authors+Show Affiliations

a Faculty of Art and Science, Department of Chemistry , Gaziosmanpasa University , Tokat , Turkey.a Faculty of Art and Science, Department of Chemistry , Gaziosmanpasa University , Tokat , Turkey.a Faculty of Art and Science, Department of Chemistry , Gaziosmanpasa University , Tokat , Turkey.b Faculty of Natural Sciences, Department of Chemistry , Cankiri Karatekin University , Cankiri , Turkey.c Faculty of Art and Science, Department of Biology , Bingol University , Bingol , Turkey.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28278670

Citation

Yildiz, Ilyas, et al. "Bioactivity-guided Isolation of Flavonoids From Cynanchum Acutum L. Subsp. Sibiricum (willd.) Rech. F. and Investigation of Their Antiproliferative Activity." Natural Product Research, vol. 31, no. 22, 2017, pp. 2629-2633.
Yildiz I, Sen O, Erenler R, et al. Bioactivity-guided isolation of flavonoids from Cynanchum acutum L. subsp. sibiricum (willd.) Rech. f. and investigation of their antiproliferative activity. Nat Prod Res. 2017;31(22):2629-2633.
Yildiz, I., Sen, O., Erenler, R., Demirtas, I., & Behcet, L. (2017). Bioactivity-guided isolation of flavonoids from Cynanchum acutum L. subsp. sibiricum (willd.) Rech. f. and investigation of their antiproliferative activity. Natural Product Research, 31(22), 2629-2633. https://doi.org/10.1080/14786419.2017.1289201
Yildiz I, et al. Bioactivity-guided Isolation of Flavonoids From Cynanchum Acutum L. Subsp. Sibiricum (willd.) Rech. F. and Investigation of Their Antiproliferative Activity. Nat Prod Res. 2017;31(22):2629-2633. PubMed PMID: 28278670.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bioactivity-guided isolation of flavonoids from Cynanchum acutum L. subsp. sibiricum (willd.) Rech. f. and investigation of their antiproliferative activity. AU - Yildiz,Ilyas, AU - Sen,Ozkan, AU - Erenler,Ramazan, AU - Demirtas,Ibrahim, AU - Behcet,Lutfi, Y1 - 2017/02/14/ PY - 2017/3/11/pubmed PY - 2018/2/7/medline PY - 2017/3/11/entrez KW - Cynanchum acutum subsp. sibiricum KW - antiproliferative activity KW - flavonoids SP - 2629 EP - 2633 JF - Natural product research JO - Nat Prod Res VL - 31 IS - 22 N2 - Cynanchum acutum L. subsp. sibiricum (Willd.) Rech. f. was extracted with hexane, acetone, methanol and water individually. A sample was heated in water then extracted with ethyl acetate. Among the extracts, the ethyl acetate extract exhibited the most antiproliferative activity, so isolation of bioactive compounds was carried out from this extract. A new compound, kaempferol-3-O-β-xylopyranosyl-(1-2)-β-rhamnopyranoside (1) along with five known compounds, quercetin-3-O-β-xyloside (2), kaempferol-3-O-β-glucoside (3), quercetin-3-O-β-glucoside (4), kaempferol-3-O-β-rhamnopyranoside (5), and kaempferol-3-O-β-d-neohesperidoside (6) were isolated from ethyl acetate extract. The structures were elucidated by spectroscopic techniques, basically 1D NMR, 2D NMR and LC-TOF/MS. Antiproliferative effects of isolated compounds were determined by xCELLigence using the HeLa (human uterus carcinoma) cell lines. Compound 2 and compound 5 revealed the good antiproliferative activity against HeLa cell lines. SN - 1478-6427 UR - https://www.unboundmedicine.com/medline/citation/28278670/Bioactivity_guided_isolation_of_flavonoids_from_Cynanchum_acutum_L__subsp__sibiricum__willd___Rech__f__and_investigation_of_their_antiproliferative_activity_ L2 - https://www.tandfonline.com/doi/full/10.1080/14786419.2017.1289201 DB - PRIME DP - Unbound Medicine ER -