Tags

Type your tag names separated by a space and hit enter

Oral delivery of double-stranded RNAs induces mortality in nymphs and adults of the Asian citrus psyllid, Diaphorina citri.
PLoS One. 2017; 12(3):e0171847.Plos

Abstract

The Asian citrus psyllid (ACP), Diaphorina citri Kuwayama, is one of the most important citrus pests. ACP is the vector of the phloem-limited bacteria Candidatus Liberibacter americanus and Candidatus Liberibacter asiaticus, the causal agents of the devastating citrus disease huanglongbing (HLB). The management of HLB is based on the use of healthy young plants, eradication of infected plants and chemical control of the vector. RNA interference (RNAi) has proven to be a promising tool to control pests and explore gene functions. Recently, studies have reported that target mRNA knockdown in many insects can be induced through feeding with double-stranded RNA (dsRNA). In the current study, we targeted the cathepsin D, chitin synthase and inhibitor of apoptosis genes of adult and nymph ACP by feeding artificial diets mixed with dsRNAs and Murraya paniculata leaves placed in dsRNAs solutions, respectively. Adult ACP mortality was positively correlated with the amount of dsRNA used. Both nymphs and adult ACP fed dsRNAs exhibited significantly increased mortality over time compared with that of the controls. Moreover, qRT-PCR analysis confirmed the dsRNA-mediated RNAi effects on target mRNAs. These results showed that RNAi can be a powerful tool for gene function studies in ACP and perhaps for HLB control.

Links

PMC Free PDF
PMC Free Full Text
FREE Publisher Full Text

Authors+Show Affiliations

Galdeano DM
Laboratório de Biotecnologia, Centro de Citricultura Sylvio Moreira, Instituto Agronômico de Campinas, Cordeirópolis, São Paulo, Brazil. Instituto de Biologia, Universidade Estadual de Campinas, Campinas, São Paulo, Brazil.
Breton MC
Laboratório de Biotecnologia, Centro de Citricultura Sylvio Moreira, Instituto Agronômico de Campinas, Cordeirópolis, São Paulo, Brazil.
Lopes JR
Escola Superior de Agricultura Luiz de Queiróz, Universidade de São Paulo, Piracicaba, São Paulo, Brazil.
Falk BW
Plant Pathology Department, University of California Davis, Davis, California, United States of America.
Machado MA
Laboratório de Biotecnologia, Centro de Citricultura Sylvio Moreira, Instituto Agronômico de Campinas, Cordeirópolis, São Paulo, Brazil.

MeSH

Administration, OralAnimalsCathepsin DChitin SynthaseCitrusHemipteraInhibitor of Apoptosis ProteinsInsect ProteinsNymphPlant DiseasesRNA InterferenceRNA, Double-StrandedRNA, MessengerReal-Time Polymerase Chain Reaction

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28282380

Citation

Galdeano, Diogo Manzano, et al. "Oral Delivery of Double-stranded RNAs Induces Mortality in Nymphs and Adults of the Asian Citrus Psyllid, Diaphorina Citri." PloS One, vol. 12, no. 3, 2017, pp. e0171847.
Galdeano DM, Breton MC, Lopes JR, et al. Oral delivery of double-stranded RNAs induces mortality in nymphs and adults of the Asian citrus psyllid, Diaphorina citri. PLoS One. 2017;12(3):e0171847.
Galdeano, D. M., Breton, M. C., Lopes, J. R., Falk, B. W., & Machado, M. A. (2017). Oral delivery of double-stranded RNAs induces mortality in nymphs and adults of the Asian citrus psyllid, Diaphorina citri. PloS One, 12(3), e0171847. https://doi.org/10.1371/journal.pone.0171847
Galdeano DM, et al. Oral Delivery of Double-stranded RNAs Induces Mortality in Nymphs and Adults of the Asian Citrus Psyllid, Diaphorina Citri. PLoS One. 2017;12(3):e0171847. PubMed PMID: 28282380.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Oral delivery of double-stranded RNAs induces mortality in nymphs and adults of the Asian citrus psyllid, Diaphorina citri. AU - Galdeano,Diogo Manzano, AU - Breton,Michèle Claire, AU - Lopes,João Roberto Spotti, AU - Falk,Bryce W, AU - Machado,Marcos Antonio, Y1 - 2017/03/10/ PY - 2016/08/01/received PY - 2017/01/26/accepted PY - 2017/3/11/entrez PY - 2017/3/11/pubmed PY - 2017/9/2/medline SP - e0171847 EP - e0171847 JF - PloS one JO - PLoS One VL - 12 IS - 3 N2 - The Asian citrus psyllid (ACP), Diaphorina citri Kuwayama, is one of the most important citrus pests. ACP is the vector of the phloem-limited bacteria Candidatus Liberibacter americanus and Candidatus Liberibacter asiaticus, the causal agents of the devastating citrus disease huanglongbing (HLB). The management of HLB is based on the use of healthy young plants, eradication of infected plants and chemical control of the vector. RNA interference (RNAi) has proven to be a promising tool to control pests and explore gene functions. Recently, studies have reported that target mRNA knockdown in many insects can be induced through feeding with double-stranded RNA (dsRNA). In the current study, we targeted the cathepsin D, chitin synthase and inhibitor of apoptosis genes of adult and nymph ACP by feeding artificial diets mixed with dsRNAs and Murraya paniculata leaves placed in dsRNAs solutions, respectively. Adult ACP mortality was positively correlated with the amount of dsRNA used. Both nymphs and adult ACP fed dsRNAs exhibited significantly increased mortality over time compared with that of the controls. Moreover, qRT-PCR analysis confirmed the dsRNA-mediated RNAi effects on target mRNAs. These results showed that RNAi can be a powerful tool for gene function studies in ACP and perhaps for HLB control. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/28282380/Oral_delivery_of_double_stranded_RNAs_induces_mortality_in_nymphs_and_adults_of_the_Asian_citrus_psyllid_Diaphorina_citri_ L2 - https://dx.plos.org/10.1371/journal.pone.0171847 DB - PRIME DP - Unbound Medicine ER -